Mosmann Lab
Welcome to the Mosmann Lab
Batch effects in flow cytometry data
Our lab has had a long-standing interest in T cell responses, particularly human, and one of our main analytical tools has been high-dimensional flow cytometry. Extraction of full information from these rich datasets was very difficult by traditional manual analysis, and this led us to develop new tools for the primary clustering of flow cytometry data into multi-dimensional cell sub-populations. In turn, the richness of even this processed data was difficult to grasp using conventional analysis methods, so we have developed several computational tools to facilitate rapid exploration of the cluster data.
Current projects include further developing our flow analysis algorithms, particularly our new Registration tool, and adapting our tools to function in the IMMPORT Galaxy website. We are also applying our tools for deep analysis of pregnancy-induced immune state changes in T cells specific for pathogens, and for paternal antigens expressed on the fetus. Additional projects explore T cell changes and signatures in projects such as Atopic Dermatitis patients responding to new biologicals, and during cancer therapy. We also visualize stochastic effects on T cell differentiation in a landscape motif, to provoke discussion of potentially novel differentiation strategies.
Tim R. Mosmann, Ph.D.
Principal Investigator
Affiliations
- Microbiology & Immunology
- Autoimmunity Centers of Excellence
- Center for AIDS Research
- Center for Biomedical Informatics
- David H. Smith Center for Vaccine Biology and Immunology
- James P. Wilmot Cancer Center
- NIH T32 Training Grant in Immunology
- Rochester Human Immunology Center
- Immunology, Microbiology, and Virology Ph.D. Program
Selected Publications
- Mosmann TR, Naim I, Rebhahn J, Datta S, Cavenaugh JS, Weaver JM, Sharma G. SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, Part 2: Biological evaluation. Cytometry A. 2014;85(5):422-33. doi: 10.1002/cyto.a.22445. PubMed PMID: 24532172; PMCID: 4238823.
- Rebhahn JA, Roumanes DR, Qi Y, Khan A, Thakar J, Rosenberg A, Lee FE, Quataert SA, Sharma G, Mosmann TR. Competitive SWIFT cluster templates enhance detection of aging changes. Cytometry A. 2016;89(1):59-70. doi: 10.1002/cyto.a.22740. PubMed PMID: 26441030; PMCID: PMC4737406.
- Aghaeepour N, Finak G, The?FlowCAP?Consortium, The?DREAM?Consortium, Hoos H, Mosmann TR, Brinkman R, Gottardo R, Scheuermann RH. Critical assessment of automated flow cytometry data analysis techniques. Nat Methods. 2013;10(3):228-38. Epub 2013/02/12. doi: 10.1038/nmeth.2365. PubMed PMID: 23396282; PMCID: 3906045.
- Mosmann TR. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63. PubMed PMID: 6606682.
- Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986;136(7):2348-57. PubMed PMID: 2419430.
- Moore KW, Vieira P, Fiorentino DF, Trounstine ML, Khan TA, Mosmann TR. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein- Barr virus gene BCRFI. Science. 1990;248(4960):1230-4. PubMed PMID: 0002161559.
- Divekar AA, Zaiss DM, Lee FE, Liu D, Topham DJ, Sijts AJ, Mosmann TR. Protein vaccines induce uncommitted IL-2-secreting human and mouse CD4 T cells, whereas infections induce more IFN-gamma-secreting cells. J Immunol. 2006;176(3):1465-73. PubMed PMID: 16424174.
- Zaiss DM, Yang L, Shah PR, Kobie JJ, Urban JF, Mosmann TR. Amphiregulin, a TH2 cytokine enhancing resistance to nematodes. Science. 2006;314(5806):1746. PubMed PMID: 17170297.
- Krishnan L, Guilbert LJ, Wegmann TG, Belosevic M, Mosmann TR. T helper 1 response against Leishmania major in pregnant C57BL/6 mice increases implantation failure and fetal resorptions. Correlation with increased IFN-gamma and TNF and reduced IL-10 production by placental cells. J Immunol. 1996;156(2):653-62. PubMed PMID: 8543817.
- Wegmann TG, Lin H, Guilbert L, Mosmann TR. Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon? Immunol Today. 1993;14(7):353-6. PubMed PMID: 8363725.
- Rebhahn JA, Deng N, Sharma G, Livingstone AM, Huang S, Mosmann TR. An animated landscape representation of CD4 T-cell differentiation, variability and plasticity: Insights into the behavior of populations versus cells. Eur J Immunol. 2014. doi: 10.1002/eji.201444645. PubMed PMID: 24945794; PMCID: 4209377.
Contact Us
Mosmann Lab
KMRB 3-9872
601 Elmwood Ave,
Rochester, NY 14642
(585) 273-1434