URMC / Labs / Frelinger Lab / Publications Publications Kearns NNorville KFrelinger JG Instant Pot for antigen retrieval: a simple, safe and economical method for use in immunohistochemistry.; BioTechniques. 2023 May 18. Norville KSkrombolas DFerry SLKearns NFrelinger JG A Protease Activatable Interleukin-2 Fusion Protein Engenders Antitumor Immune Responses by Interferon Gamma-Dependent and Interferon Gamma-Independent Mechanisms.; Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research; Vol 42(7), pp. 316-328. 2022 Jul. Bottaro ABrown DMFrelinger JG Editorial: The Present and Future of Immunology Education.; Frontiers in immunology; Vol 12. 2021. Skrombolas DSullivan MFrelinger JG Development of an Interleukin-12 Fusion Protein That Is Activated by Cleavage with Matrix Metalloproteinase 9.; Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 2019 Mar 08. Haidaris CGFrelinger JG Inoculating a New Generation: Immunology in Medical Education.; Frontiers in immunology; Vol 10. 2019. Mikucki MESkitzki JJFrelinger JGOdunsi KGajewski TFLuster ADEvans SS Unlocking tumor vascular barriers with CXCR3: Implications for cancer immunotherapy.; Oncoimmunology; Vol 5(5). 2016 May. Skrombolas DWylie IMaharaj SFrelinger JG Characterization of an IL-12 p40/p35 Truncated Fusion Protein That can Inhibit the Action of IL-12.; Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research; Vol 35(9). 2015 Sep. Mikucki MEFisher DTMatsuzaki JSkitzki JJGaulin NBMuhitch JBKu AWFrelinger JGOdunsi KGajewski TFLuster ADEvans SS Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints.; Nature communications; Vol 6. 2015. Skrombolas DFrelinger JG Challenges and developing solutions for increasing the benefits of IL-2 treatment in tumor therapy.; Expert review of clinical immunology; Vol 10(2). 2014 Feb. Sedlacek ALGerber SARandall TDvan Rooijen NFrelinger JGLord EM Generation of a dual-functioning antitumor immune response in the peritoneal cavity.; The American journal of pathology; Vol 183(4). 2013 Oct. Gerber SASedlacek ALCron KRMurphy SPFrelinger JGLord EM IFN-γ mediates the antitumor effects of radiation therapy in a murine colon tumor.; The American journal of pathology; Vol 182(6). 2013 Jun. Gerber SASorensen EWSedlacek ALLim JYSkrombolas DFrelinger JGLord EM Local expression of interleukin-2 by B16 melanoma cells results in decreased tumour growth and long-term tumour dormancy.; Immunology; Vol 138(3). 2013 Mar. Yokota SJFacciponte JGKelleher RJShultz LDLoyall JLParsons RROdunsi KFrelinger JGLord EMGerber SABalu-Iyer SVBankert RB Changes in ovarian tumor cell number, tumor vasculature, and T cell function monitored in vivo using a novel xenograft model.; Cancer immunity; Vol 13. 2013. Valentino MDAbdul-Alim CSMaben ZJSkrombolas DHensley LLKawula THDziejman MLord EMFrelinger JAFrelinger JG A broadly applicable approach to T cell epitope identification: application to improving tumor associated epitopes and identifying epitopes in complex pathogens.; Journal of immunological methods; Vol 373(1-2). 2011 Oct 28. Puskas JSkrombolas DSedlacek ALord ESullivan MFrelinger J Development of an attenuated interleukin-2 fusion protein that can be activated by tumour-expressed proteases.; Immunology; Vol 133(2). 2011 Jun. Valentino MDMaben ZJHensley LLWoolard MDKawula THFrelinger JAFrelinger JG Identification of T-cell epitopes in Francisella tularensis using an ordered protein array of serological targets.; Immunology; Vol 132(3). 2011 Mar. Valentino MDAbdul-Alim CSMaben ZJSkrombolas DHensley LLKawula THDziejman MLord EMFrelinger JAFrelinger JG A broadly applicable approach to T cell epitope identification: Application to improving tumor associated epitopes and identifying epitopes in complex pathogens; Journal of Immunological Methods (in press). 2011 Jan 01. Sorensen EWGerber SAFrelinger JGLord EM IL-12 suppresses vascular endothelial growth factor receptor 3 expression on tumor vessels by two distinct IFN-gamma-dependent mechanisms.; Journal of immunology (Baltimore, Md. : 1950); Vol 184(4). 2010 Feb 15. Valentino MFrelinger J An approach to the identification of T cell epitopes in the genomic era: application to Francisella tularensis.; Immunologic research; Vol 45(2-3). 2009 Dec. Valentino MDHensley LLSkrombolas DMcPherson PLWoolard MDKawula THFrelinger JAFrelinger JG Identification of a dominant CD4 T cell epitope in the membrane lipoprotein Tul4 from Francisella tularensis LVS.; Molecular immunology; Vol 46(8-9). 2009 May. Lugade AASorensen EWGerber SAMoran JPFrelinger JGLord EM Radiation-induced IFN-gamma production within the tumor microenvironment influences antitumor immunity.; Journal of immunology (Baltimore, Md. : 1950); Vol 180(5). 2008 Mar 1. Malboeuf CMSimon DALee YELankes HADewhurst SFrelinger JGRose RC Human papillomavirus-like particles mediate functional delivery of plasmid DNA to antigen presenting cells in vivo.; Vaccine; Vol 25(17). 2007 Apr 30. Santos KDuke CMRodriguez-Colon SMDakwar AFan SKeefer MCFederoff HJFrelinger JGBowers WJDewhurst S Effect of promoter strength on protein expression and immunogenicity of an HSV-1 amplicon vector encoding HIV-1 Gag.; Vaccine; Vol 25(9). 2007 Feb 19. Santos KSimon DAConway EBowers WJMitra SFoster THLugade ALord EMFederoff HJDewhurst SFrelinger JG Spatial and temporal expression of herpes simplex virus type 1 amplicon-encoded genes: implications for their use as immunization vectors.; Human gene therapy; Vol 18(2). 2007 Feb. Ramanayake TSimon DAFrelinger JGLord EMRobert J In vivo study of T-cell responses to skin alloantigens in Xenopus using a novel whole-mount immunohistology method.; Transplantation; Vol 83(2). 2007 Jan 27. Mitra SCassar SENiles DJPuskas JAFrelinger JGFoster TH Photodynamic therapy mediates the oxygen-independent activation of hypoxia-inducible factor 1alpha.; Molecular cancer therapeutics; Vol 5(12). 2006 Dec. Gerber SARybalko VYBigelow CELugade AAFoster THFrelinger JGLord EM Preferential attachment of peritoneal tumor metastases to omental immune aggregates and possible role of a unique vascular microenvironment in metastatic survival and growth.; The American journal of pathology; Vol 169(5). 2006 Nov. Wagner VEFrelinger JGBarth RKIglewski BH Quorum sensing: dynamic response of Pseudomonas aeruginosa to external signals.; Trends in microbiology; Vol 14(2). 2006 Feb. Lugade AAMoran JPGerber SARose RCFrelinger JGLord EM Local radiation therapy of B16 melanoma tumors increases the generation of tumor antigen-specific effector cells that traffic to the tumor.; Journal of immunology (Baltimore, Md. : 1950); Vol 174(12). 2005 Jun 15. Gerber SATurner MJLugade AAMoran JPFrelinger JGLord EM Characterization of a lymph node within the mouse prostate: Detailed analysis using whole mount histology.; The Prostate; Vol 63(2). 2005 May 1. Bigelow CEVishwasrao HDFrelinger JGFoster TH Imaging enzyme activity with polarization-sensitive confocal fluorescence microscopy.; Journal of microscopy; Vol 215(Pt 1). 2004 Jul. Mitra SGoren EMFrelinger JGFoster TH Activation of heat shock protein 70 promoter with meso-tetrahydroxyphenyl chlorin photodynamic therapy reported by green fluorescent protein in vitro and in vivo.; Photochemistry and photobiology; Vol 78(6). 2003 Dec. Moran JPGerber SAMartin CAFrelinger JGLord EM Transfection of the genes for interleukin-12 into the K1735 melanoma and the EMT6 mammary sarcoma murine cell lines reveals distinct mechanisms of antitumor activity.; International journal of cancer; Vol 106(5). 2003 Sep 20. Gerber SAMoran JPFrelinger JGFrelinger JAFenton BMLord EM Mechanism of IL-12 mediated alterations in tumour blood vessel morphology: analysis using whole-tissue mounts.; British journal of cancer; Vol 88(9). 2003 May 6. Fisher TLNocera MWillis RATurner MJAbdul Alim CSBrown DMBourne PAdi Sant'Agnese PAMessing EMLord EMFrelinger JG Generation of monoclonal antibodies specific for human kallikrein 2 (hK2) using hK2-expressing tumors.; The Prostate; Vol 51(3). 2002 May 15. Patel DFrelinger JGoudsmit JKim B In vitro assay for site-specific proteases using bead-attached GFP substrate.; BioTechniques; Vol 31(5). 2001 Nov. Willis RABowers WJTurner MJFisher TLAbdul-Alim CSHoward DFFederoff HJLord EMFrelinger JG Dendritic cells transduced with HSV-1 amplicons expressing prostate-specific antigen generate antitumor immunity in mice.; Human gene therapy; Vol 12(15). 2001 Oct 10. Turner MJAbdul-Alim CSWillis RAFisher TLLord EMFrelinger JG T-cell antigen discovery (T-CAD) assay: a novel technique for identifying T cell epitopes.; Journal of immunological methods; Vol 256(1-2). 2001 Oct 1. Brown DMFisher TLWei CFrelinger JGLord EM Tumours can act as adjuvants for humoral immunity.; Immunology; Vol 102(4). 2001 Apr. Skrincosky DWillis RAHocknell PKFrelinger JGMirandola PWang XDewhurst S Epitope mapping of human herpesvirus-7 gp65 using monoclonal antibodies.; Archives of virology; Vol 146(9). 2001. Egan RMYorkey CBlack RLoh WKStevens JLStorozynsky ELord EMFrelinger JGWoodward JG In vivo behavior of peptide-specific T cells during mucosal tolerance induction: antigen introduced through the mucosa of the conjunctiva elicits prolonged antigen-specific T cell priming followed by anergy.; Journal of immunology (Baltimore, Md. : 1950); Vol 164(9). 2000 May 01. Lee JMoran JPFenton BMKoch CJFrelinger JGKeng PCLord EM Alteration of tumour response to radiation by interleukin-2 gene transfer.; British journal of cancer; Vol 82(4). 2000 Feb. Conway TFSabel MSSugano MFrelinger JGEgilmez NKChen FBankert RB Growth of human tumor xenografts in SCID mice quantified using an immunoassay for tumor marker protein in serum.; Journal of immunological methods; Vol 233(1-2). 2000 Jan 13. Martin BKFrelinger JGTing JP Combination gene therapy with CD86 and the MHC class II transactivator in the control of lung tumor growth.; Journal of immunology (Baltimore, Md. : 1950); Vol 162(11). 1999 Jun 1. Storozynsky EWoodward JGFrelinger JGLord EM Interleukin-3 and granulocyte-macrophage colony-stimulating factor enhance the generation and function of dendritic cells.; Immunology; Vol 97(1). 1999 May. Ostberg JRBarth RKFrelinger JG The Roman god Janus: a paradigm for the function of CD43.; Immunology today; Vol 19(12). 1998 Dec. Wei CCallahan BPTurner MJWillis RALord EMBarth RKFrelinger JG Regulation of human prostate-specific antigen gene expression in transgenic mice: evidence for an enhancer between the PSA and human glandular kallikrein-1 genes.; International journal of molecular medicine; Vol 2(4). 1998 Oct. Yeh KYMcAdam AJPulaski BAShastri NFrelinger JGLord EM IL-3 enhances both presentation of exogenous particulate antigen in association with class I major histocompatibility antigen and generation of primary tumor-specific cytolytic T lymphocytes.; Journal of immunology (Baltimore, Md. : 1950); Vol 160(12). 1998 Jun 15. Lord EMYeh KYMoran JAStorozynsky EFrelinger JG IL-3-mediated enhancement of particulate antigen presentation by macrophages.; Journal of immunotherapy (Hagerstown, Md. : 1997); Vol 21(3). 1998 May. Lord EMFrelinger JG Tumor immunotherapy: cytokines and antigen presentation.; Cancer immunology, immunotherapy : CII; Vol 46(2). 1998 Apr. Lee JFenton BMKoch CJFrelinger JGLord EM Interleukin 2 expression by tumor cells alters both the immune response and the tumor microenvironment.; Cancer research; Vol 58(7). 1998 Apr 1. Willis RAWei CTurner MJCallahan BPPugh AEBarth RKLord EMFrelinger JG A transgenic strategy for analyzing the regulatory regions of the human prostate-specific antigen gene: potential applications for the treatment of prostate cancer (Review).; International journal of molecular medicine; Vol 1(2). 1998 Feb. Ostberg JRDragone LLBorrello MAPhipps RPBarth RKFrelinger JG Expression of mouse CD43 in the B cell lineage of transgenic mice causes impaired immune responses to T-independent antigens.; European journal of immunology; Vol 27(9). 1997 Sep. Wei CWillis RATilton BRLooney RJLord EMBarth RKFrelinger JG Tissue-specific expression of the human prostate-specific antigen gene in transgenic mice: implications for tolerance and immunotherapy.; Proceedings of the National Academy of Sciences of the United States of America; Vol 94(12). 1997 Jun 10. McEvoy LMSun HFrelinger JGButcher EC Anti-CD43 inhibition of T cell homing.; The Journal of experimental medicine; Vol 185(8). 1997 Apr 21. Ostberg JRDragone LLDriskell TMoynihan JAPhipps RBarth RKFrelinger JG Disregulated expression of CD43 (leukosialin, sialophorin) in the B cell lineage leads to immunodeficiency.; Journal of immunology (Baltimore, Md. : 1950); Vol 157(11). 1996 Dec 1. Wei CStorozynsky EMcAdam AJYeh KYTilton BRWillis RABarth RKLooney RJLord EMFrelinger JG Expression of human prostate-specific antigen (PSA) in a mouse tumor cell line reduces tumorigenicity and elicits PSA-specific cytotoxic T lymphocytes.; Cancer immunology, immunotherapy : CII; Vol 42(6). 1996 Jul. Pulaski BAYeh KYShastri NMaltby KMPenney DPLord EMFrelinger JG Interleukin 3 enhances cytotoxic T lymphocyte development and class I major histocompatibility complex "re-presentation" of exogenous antigen by tumor-infiltrating antigen-presenting cells.; Proceedings of the National Academy of Sciences of the United States of America; Vol 93(8). 1996 Apr 16. McAdam AJPulaski BAStorozynsky EYeh KYSickel JZFrelinger JGLord EM Analysis of the effect of cytokines (interleukins 2, 3, 4, and 6, granulocyte-monocyte colony-stimulating factor, and interferon-gamma) on generation of primary cytotoxic T lymphocytes against a weakly immunogenic tumor.; Cellular immunology; Vol 165(2). 1995 Oct 15. Yeh KYPulaski BAWoods MLMcAdam AJGaspari AAFrelinger JGLord EM B7-1 enhances natural killer cell-mediated cytotoxicity and inhibits tumor growth of a poorly immunogenic murine carcinoma.; Cellular immunology; Vol 165(2). 1995 Oct 15. McAdam AJPulaski BAHarkins SSHutter EKLord EMFrelinger JG Synergistic effects of co-expression of the TH1 cytokines IL-2 and IFN-gamma on generation of murine tumor-reactive cytotoxic cells.; International journal of cancer; Vol 61(5). 1995 May 29. Dragone LLBarth RKSitar KLDisbrow GLFrelinger JG Disregulation of leukosialin (CD43, Ly48, sialophorin) expression in the B-cell lineage of transgenic mice increases splenic B-cell number and survival.; Proceedings of the National Academy of Sciences of the United States of America; Vol 92(2). 1995 Jan 17. McAdam AJFelcher AWoods MLPulaski BAHutter EKFrelinger JGLord EM Transfection of transforming growth factor-beta producing tumor EMT6 with interleukin-2 elicits tumor rejection and tumor reactive cytotoxic T-lymphocytes.; Journal of immunotherapy with emphasis on tumor immunology : official journal of the Society for Biological Therapy; Vol 15(3). 1994 Apr. Woods MLMcAdam AJFrelinger JGLord EM Isolation and expansion of tumor-infiltrating lymphocytes.; BioTechniques; Vol 15(6). 1993 Dec. McAdam AJYeh KYPulaski BAFrelinger JGLord EM Interleukin-3 inhibits the generation of nonspecific killers by interleukin-2.; Journal of immunotherapy with emphasis on tumor immunology : official journal of the Society for Biological Therapy; Vol 14(4). 1993 Nov. Pulaski BAMcAdam AJHutter EKBiggar SLord EMFrelinger JG Interleukin 3 enhances development of tumor-reactive cytotoxic cells by a CD4-dependent mechanism.; Cancer research; Vol 53(9). 1993 May 1. Gaspari AAFerbel BFrelinger JG Thy 1+ dendritic epidermal cells but not Langerhans cells express Ly 48.; The Journal of investigative dermatology; Vol 100(3). 1993 Mar. Baecher-Allan CMKemp JDDorfman KSBarth RKFrelinger JG Differential epitope expression of Ly-48 (mouse leukosialin).; Immunogenetics; Vol 37(3). 1993. McAdam AJBlieden TMFrelinger JGLord EM CD4-class II major histocompatibility complex interaction does not enhance killing by a class I-restricted CD4+CD8+ cytotoxic T cell clone.; European journal of immunology; Vol 22(9). 1992 Sep. Cerosaletti KMWoodward JGLord EMFrelinger JG Two regions of the H-2 Dd promoter are responsive to dimethylsulfoxide in line 1 cells by a mechanism distinct from IFN-gamma.; Journal of immunology (Baltimore, Md. : 1950); Vol 148(4). 1992 Feb 15. Dragone LPallant AInsel RFrelinger JG CD43 is expressed normally on Wiskott-Aldrich-derived lymphocytes.; Experimental and clinical immunogenetics; Vol 9(3). 1992. Blieden TMMcAdam AJFrelinger JGLord EM Mechanism of cytolytic T lymphocyte killing of a low class I-expressing tumor.; Journal of immunology (Baltimore, Md. : 1950); Vol 147(4). 1991 Aug 15. Blieden TMMcAdam AJForesman MDCerosaletti KMFrelinger JGLord EM Class-I MHC expression in the mouse lung carcinoma, line 1: a model for class-I inducible tumors.; International journal of cancer. Supplement = Journal international du cancer. Supplement; Vol 6. 1991. Dorfman KSLitaker WBaecher CMFrelinger JG The nucleotide sequence of Ly 48 (mouse leukosialin, sialophorin): the mouse homolog of CD43.; Nucleic acids research; Vol 18(16). 1990 Aug 25. Phipps RPBaecher CFrelinger JGPenney DPKeng PBrown D Differential expression of interleukin 1 alpha by Thy-1+ and Thy-1- lung fibroblast subpopulations: enhancement of interleukin 1 alpha production by tumor necrosis factor-alpha.; European journal of immunology; Vol 20(8). 1990 Aug. Pallant AFukuda MFrelinger JG CD43 (leukosialin, sialophorin, large sialoglycoprotein) can be expressed in both normal and Wiskott-Aldrich fibroblasts via transfection of a leukosialin cDNA.; European journal of immunology; Vol 20(7). 1990 Jul. Cerosaletti KMBlieden TMHarwell LWWelsh KMFrelinger JGLord EM Alteration of the metastatic potential of line 1 lung carcinoma cells: opposite effects of class I antigen induction by interferons versus DMSO or gene transfection.; Cellular immunology; Vol 127(2). 1990 May. Baecher CMDorfman KSMattei MGFrelinger JG cDNA cloning and localization of the mouse leukosialin gene (Ly48) to chromosome 7.; Immunogenetics; Vol 31(5-6). 1990. Pallant AEskenazi AMattei MGFournier RECarlsson SRFukuda MFrelinger JG Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16.; Proceedings of the National Academy of Sciences of the United States of America; Vol 86(4). 1989 Feb. Bahler DWCerosaletti KMLord EMFrelinger JG Molecular analysis of deficient class I H-2 antigen expression by mouse lung carcinoma cells.; Journal of immunology (Baltimore, Md. : 1950); Vol 140(11). 1988 Jun 1. Dennert GLandon CLord EMBahler DWFrelinger JG Lysis of a lung carcinoma by poly I:C-induced natural killer cells is independent of the expression of class I histocompatibility antigens.; Journal of immunology (Baltimore, Md. : 1950); Vol 140(7). 1988 Apr 1. Baecher CMInfante AJSemcheski KLFrelinger JG Identification and characterization of a mouse cell surface antigen with alternative molecular forms.; Immunogenetics; Vol 28(5). 1988. Bahler DWFrelinger JGHarwell LWLord EM Reduced tumorigenicity of a spontaneous mouse lung carcinoma following H-2 gene transfection.; Proceedings of the National Academy of Sciences of the United States of America; Vol 84(13). 1987 Jul. Sawicki JAFrelinger JGPalmer MBuck DADietzschold BWettstein PJ Identical RT1 class II molecules are expressed by rat RT1m and RT1c haplotypes.; Journal of immunology (Baltimore, Md. : 1950); Vol 135(4). 1985 Oct. Frelinger JGShigeta MFathman CG An analysis of monoclonal T cell and antibody recognition sites on Ia molecules.; The Journal of investigative dermatology; Vol 85(1 Suppl). 1985 Jul. Frelinger JSing AInfante AFathman CG Clonotypic antibodies which stimulate T cell clone proliferation.; Immunological reviews; Vol 81. 1984 Oct. Frelinger JGShigeta MInfante AJNelson PAPierres MFathman CG Multiple functional sites on a single Ia molecule defined using T cell clones and antibodies with chain-determined specificity.; The Journal of experimental medicine; Vol 159(3). 1984 Mar 1. Fathman CGFrelinger JG T-lymphocyte clones.; Annual review of immunology; Vol 1. 1983. Beck BNFrelinger JGShigeta MInfante AJCummings DHämmerling GFathman CG T cell clones specific for hybrid I-A molecules. Discrimination with monoclonal anti-I-Ak antibodies.; The Journal of experimental medicine; Vol 156(4). 1982 Oct 1. Steinmetz MMoore KWFrelinger JGSher BTShen FWBoyse EAHood L A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains.; Cell; Vol 25(3). 1981 Sep. Frelinger JGHood LWettstein P Analyses of RT1 products using two-dimensional polyacrylamide gels.; Transplantation proceedings; Vol 13(2). 1981 Jun. Wettstein PJFrelinger JGHood L Serologic and biochemical analyses of rat class II molecules with anti-Ia sera.; Transplantation proceedings; Vol 13(2). 1981 Jun. Steinmetz MFrelinger JGFisher DHunkapiller TPereira DWeissman SMUehara HNathenson SHood L Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.; Cell; Vol 24(1). 1981 Apr. Frelinger JGFrelinger JAHood L Peptide map comparisons of epidermal and spleen H-2 molecules.; Immunogenetics; Vol 12(5-6). 1981 Mar 1. Wettstein PJFrelinger JGHood L Serological and biochemical characterization of rat (RT1) class II molecules with restricted mouse anti-Ia sera.; Immunogenetics; Vol 13(1-2). 1981. Frelinger JAFrelinger JG Bone marrow origin of Ia molecules purified from epidermal cells.; The Journal of investigative dermatology; Vol 75(1). 1980 Jul. Blankenhorn EPCecka JMFrelinger JGötze DHood L Structure of Ia antigens from the rat. Mouse alloantisera demonstrate at least two distinct molecular species.; European journal of immunology; Vol 10(2). 1980 Feb. Frelinger JGHood LHill SFrelinger JA Mouse epidermal Ia molecules have a bone marrow origin.; Nature; Vol 282(5736). 1979 Nov 15. Frelinger JGMotulsky HWoodward DO Effects of Chloramphenicol on the Circadian Rhythm of Neurospora crassa.; Plant physiology; Vol 58(4). 1976 Oct.