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Reagents available upon request
Xenopus Lymphocyte Surface Markers Detectable with Available mAbs
| Xenopus markers (mAbs) |
Expression Pattern |
CD8
(AM22, F17) |
Larval and adult thymocytes (70-80%) and T-cells (about 20% of splenocytes). All lymphoid tumor lines |
CTX
(X71, 1S9.2) |
Larval and adult thymocytes (60-70%)no consistent expression in periphery. All lymphoid tumor lines and gut epithelial tissue. |
XT1
(XT1) |
Most, but not all, larval and adult T-cells; earliest marker of thymocytes. All lymphoid tumor lines |
MHC class I
(TB17) |
Ubiquitous in adult; all lymphopoietic lineages. Not expressed until metamorphosis |
MHC class II
(AM20, 14A2) |
Thymocytes, B and T-cells (99% of spleen lymphocytes), only B-cells in larvae |
CD5
(2B1) |
Thymocytes (>95%), T-cells and some PMA-activated IgM+ B cells. All lymphoid tumor lines |
NK-like
(1F8) |
Non-B and non-T, peripheral lymphoid cells. |
| RC47 |
Leukocyte lineage from very early stage. Thymic cortex and medulla (>90% of total thymocytes) |
IgM
(10A9) |
Larval and adult B cells. |
IgY
(11D5) |
Some larval and adult B cells. |
IgX
(410D9) |
Some larval and adult B cells, especially in the gut. |
cDNA Encoding Immunologically-relevant X. laevis Genes
| Immune System Characteristics |
Larva (Ancestral-like system)
Adult (Mammalian-like system) |
| CTX |
Thymus, all lymphoid tumor lines and gut epithelial tissue. |
| TCR α, β, γ |
thymus, circulating T-cells |
| BCR μ, γ, ξ, L |
B cells |
| MHC class I |
ubiquitous in adult |
| Non-classical class Ib |
more restricted tissue distribution for some of them
MHC class II |
| MHC class II |
thymus, splenic lymphocytes |
| Rag 1 & 2 |
mainly thymus, at lower level in bone marrow, kidney, spleen and liver |
| TdT |
mainly thymus of old larvae and adult, less in spleen |
| Ikaros |
mainly thymus |
| GATA III |
thymus and T-cells |
| LMP7 |
Thymus, spleen |
| Hsps (gp96, hsp70) |
Ubiquitousely expressed |
Animals currently available (embryos, tadpoles or adults)
|
MHC homozygous strain
|
MHC haplotypes
|
|
Inbred J
|
j/j
|
|
Partially inbred F
|
f/f
|
|
F/J cross
|
f/j
|
|
Isogenetic laevis/gilli (LG) clones
|
MHC haplotypes
|
|
LG-15
|
a/c
|
|
LG-6
|
a/c
|
|
Transgenic lines
|
Genetic background
|
|
xlurp:GFP
|
Outbred and J (F3)
|
|
flk:GFP
|
Outbred
|
|
Mpeg:Cherry/xlurp-GFP
|
Outbred
|
|
Silenced b2m (shRNA)
|
Outbred and LG-15
|
|
Silenced XNC10 (shRNA)
|
Outbred and LG-15
|
|
Other Xenopus species
|
|
|
X. borealis
|
|
Cloned Thymic Tumor Cell Lines
| Tumor Cell Line |
BB7 |
ff-2 |
15/0 |
| Genetic background of initial tumor-bearing host |
Partially inbred MHC
homozygous ff strain |
LG-15 (MHC a/c) isogenetic clone |
| T-cell surface markers (CD8, CD5, pan T-cell) |
+++ |
+++ |
+++ |
| Ig mRNA |
- |
+ |
+ |
| Ig protein |
- |
- |
- |
| MHC class I mRNA and protein |
- |
+ |
- |
| MHC class II mRNA and protein |
- |
- |
- |
| Tumorigenicity in syngeneic |
| engaLarvae |
- |
+ |
++ |
| Adult |
|
|
|
Genomic and cDNA Libraries Genomic and cDNA Libraries
X. laevis cDNA libraries/Old libraries in λZAPII vector Description ST: Outbred adult spleen + thymus (constructed by L. Du Pasquier, Basel Switzerland)) LG7Ad: Adult from the clone LG7 immunized with DNP-KLH + CFA constructed L. Du Pasquier) LG7 Tadp: LG7 pre-metamorphic larvae immunized DNP-KLH + alum (constructed by M. Wilson, Jakson MS) SMART cDNA libraries
(λZAP-express, constructed by S. Guselnikov and A. Taranin,
Novosibirsk, Russia) Description T: Outbred larval spleen + thymus + liver. 7x105 independent clones, titer 6x109 S: Outbred adult spleen. 2x106 independent clones, titer 1x109 NK: NK cells, 1F8+ purified from outbred adult splenocytes. 3x106 independent clones, titer 1x1010 T: CD8 NK/T cells (AM22+/1F8+) purified from outbred splenocytes. 5x105 independent clones, titer 4x109 Arrayed EST cDNA libraries
(constructed by Express Genomics, Inc.) Outbred adult thymus
Outbred adult splenocytes activated for 4 days with PHA X. tropicalis
cDNA libraries (λZAP-express) Trop: Adults spleen + thymus + intestine. Titer 5x1010