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Eileen A. Ryan, M.D.

Eileen A. Ryan, M.D.

she/her/hers

Contact

Office (585) 275-3184

About Me

Dr. Ryan specializes in hematopathology. She is a former Director of the Hematology and Molecular Diagnostics laboratories and was Director of the URMC Labs until her appointment as Department Chair in 2004. Her research established molecular events determining B lymphoid lineage commitment and ea...
Dr. Ryan specializes in hematopathology. She is a former Director of the Hematology and Molecular Diagnostics laboratories and was Director of the URMC Labs until her appointment as Department Chair in 2004. Her research established molecular events determining B lymphoid lineage commitment and early differentiation of human B cell progenitors. She has recently focused on the elucidation of the role of common genetic variants in determining outcome of patients following a coronary event.

Faculty Appointments

Professor Emeritus - Department of Pathology and Laboratory Medicine (SMD)

Credentials

Post-doctoral Training & Residency

Resident in Pathology, University of Rochester Medical Center 1976 - 1981

Intern in Pediatrics, University of Rochester Medical Center 1975 - 1976

Education

MD | Johns Hopkins U Sch Medicine. Medicine. 1975

BS | University of Notre Dame. Pre-Medical. 1971

Awards

My Fifth Award
University of Rochester Medical Center 2000

My Fourth Award
University of Rochester Medical Center 1999

My Third Award
University of Rochester Medical Center 1998

Research

We are interested in the molecular events determining B lymphoid lineage commitment and early differentiation of B cell progenitors. Insight into the regulation of B cell development can be applied to studies of childhood lymphoblastic leukemia, a neoplasm derived from an early B lymphoid progenitor...
We are interested in the molecular events determining B lymphoid lineage commitment and early differentiation of B cell progenitors. Insight into the regulation of B cell development can be applied to studies of childhood lymphoblastic leukemia, a neoplasm derived from an early B lymphoid progenitor which cannot properly commit and/or differentiate into a mature B cell.

Signals for human B cell development are incompletely understood, largely due to difficulties in culturing normal human B cell progenitors. Using a bone marrow stromal dependent culture system, we have identified colony forming human lymphoid progenitors in a very early bone marrow population (CD34+/CD19-/IL-7 receptor -) which expresses early myeloid as well as lymphoid markers, and may therefore represent cells engaged in the earliest steps of commitment to two of the major hematopoietic lineages, lymphoid and myeloid. This experimental system can be used to define critical molecular events determining the commitment of early human hematopoietic progenitors to B lymphoid differentiation.

The goal of my research is to characterize the signals for lineage commitment and B lineage differentiation of these early human lymphoid progenitors. Efforts focus on analysis of immunoglobulin gene rearrangements of individual progenitors as an indicator of early B lineage commitment using advanced cell sorting and micromanipulation techniques, and transfer of B lineage specific regulatory genes directly into normal human hematopoietic progenitors to assess their contribution to B lineage commitment and development.

Patents

Identifying Risk of a Medical Event

Issue date: March 17, 2015

Publications

Journal Articles

Influences on plasminogen activator inhibitor-2 polymorphism-associated recurrent cardiovascular disease risk in patients with high HDL cholesterol and inflammation.

Corsetti JP, Salzman P, Ryan D, Moss AJ, Zareba W, Sparks CE

Atherosclerosis.. 2016 July 250 :1-8. Epub 04/21/2016.

A critical role for the protein kinase PKK in the maintenance of recirculating mature B cells and the development of B1 cells.

Chen L, Oleksyn D, Pulvino M, Sanz I, Ryan D, Ryan C, Lin CS, Poligone B, Pentland AP, Ritchlin C, Zhao J

Immunology letters.. 2016 April 172 :67-78. Epub 02/26/2016.

Ganglionitis and genetic cardiac arrhythmias: more questions than answers.

Moss AJ, Ryan DH, Yeaney GA

Circulation. Arrhythmia and electrophysiology.. 2014 April 7 (2):190-1. Epub 1900 01 01.

Senior academic physicians and retirement considerations.

Moss AJ, Greenberg H, Dwyer EM, Klein H, Ryan D, Francis C, Marcus F, Eberly S, Benhorin J, Bodenheimer M, Brown M, Case R, Gillespie J, Goldstein R, Haigney M, Krone R, Lichstein E, Locati E, Oakes D, Thomsen PE, Zareba W

Progress in cardiovascular diseases.. 2013 55 (6):611-5. Epub 03/21/2013.

Plasminogen activator inhibitor-2 polymorphism associates with recurrent coronary event risk in patients with high HDL and C-reactive protein levels.

Corsetti JP, Salzman P, Ryan D, Moss AJ, Zareba W, Sparks CE

PloS one.. 2013 8 (7):e68920. Epub 07/09/2013.

Thrombospondin-4 polymorphism (A387P) predicts cardiovascular risk in postinfarction patients with high HDL cholesterol and C-reactive protein levels.

Corsetti JP, Ryan D, Moss AJ, McCarthy J, Goldenberg I, Zareba W, Sparks CE

Thrombosis and haemostasis.. 2011 December 106 (6):1170-8. Epub 10/20/2011.

Glycerol as a reference material for fecal fat quantitation using low-resolution time domain ¹H NMR spectroscopy.

Corsetti JP, Sterry J, Sakpal M, Lefevre BH, Ryan D

Clinical biochemistry.. 2011 November 44 (16):1352-4. Epub 08/26/2011.

Collaborative clinical trials.

Moss AJ, Francis CW, Ryan D

The New England journal of medicine.. 2011 March 3364 (9):789-91. Epub 1900 01 01.

Cholesteryl ester transfer protein polymorphism (TaqIB) associates with risk in postinfarction patients with high C-reactive protein and high-density lipoprotein cholesterol levels.

Corsetti JP, Ryan D, Rainwater DL, Moss AJ, Zareba W, Sparks CE

Arteriosclerosis, thrombosis, and vascular biology.. 2010 August 30 (8):1657-64. Epub 05/20/2010.

The common apolipoprotein A-1 polymorphism -75A>G is associated with ethnic differences in recurrent coronary events after recovery from an acute myocardial infarction.

Block R, Corsetti J, Goldenberg I, Vorobiof G, McNitt S, Ryan D, Zareba W, Moss AJ

Heart international. 2009 June 304 (1):e8. Epub 1900 01 01.

Lp(a) and risk of recurrent cardiac events in obese postinfarction patients.

Corsetti JP, Ryan D, Rainwater DL, Moss AJ, Zareba W, Block RC, Sparks CE

Obesity.. 2008 December 16 (12):2717-22. Epub 10/16/2008.

Cumulative burden of atherosclerotic risk genotypes and the age at onset of a first myocardial infarction: a case-only carriership approach.

Goldenberg I, Moss AJ, Ryan D, Pietrasik G, Zareba W, McNitt S, Eberly SW

Annals of noninvasive electrocardiology : the official journal of the International Society for Holter and Noninvasive Electrocardiology, Inc.. 2008 July 13 (3):287-94. Epub 1900 01 01.

Plasminogen activator inhibitor-1 polymorphism (4G/5G) predicts recurrence in nonhyperlipidemic postinfarction patients.

Corsetti JP, Ryan D, Moss AJ, Rainwater DL, Zareba W, Sparks CE

Arteriosclerosis, thrombosis, and vascular biology.. 2008 March 28 (3):548-54. Epub 12/20/2007.

NAD(P)H oxidase polymorphism (C242T) and high HDL cholesterol associate with recurrent coronary events in postinfarction patients.

Corsetti JP, Ryan D, Moss AJ, Zareba W, Sparks CE

Atherosclerosis.. 2008 January 196 (1):461-468. Epub 01/09/2007.

Polymorphism in the cholesteryl ester transfer protein gene and the risk of early onset myocardial infarction among cigarette smokers.

Goldenberg I, Moss AJ, Block R, Ryan D, Corsetti JP, McNitt S, Eberly SW, Zareba W

Annals of noninvasive electrocardiology : the official journal of the International Society for Holter and Noninvasive Electrocardiology, Inc.. 2007 October 12 (4):364-74. Epub 1900 01 01.

Glycoprotein Ibalpha polymorphism T145M, elevated lipoprotein-associated phospholipase A2, and hypertriglyceridemia predict risk for recurrent coronary events in diabetic postinfarction patients.

Corsetti JP, Ryan D, Moss AJ, Rainwater DL, Zareba W, Sparks CE

Diabetes.. 2007 May 56 (5):1429-35. Epub 02/15/2007.

Polymorphisms in the paraoxonase and endothelial nitric oxide synthase genes and the risk of early-onset myocardial infarction.

Morray B, Goldenberg I, Moss AJ, Zareba W, Ryan D, McNitt S, Eberly SW, Glazko G, Mathew J

The American journal of cardiology.. 2007 April 1599 (8):1100-5. Epub 02/21/2007.

Polymorphism in the angiotensinogen gene, hypertension, and ethnic differences in the risk of recurrent coronary events.

Goldenberg I, Moss AJ, Ryan D, McNitt S, Eberly SW, Zareba W

Hypertension.. 2006 October 48 (4):693-9. Epub 08/28/2006.

Atherosclerotic risk genotypes and recurrent coronary events after myocardial infarction.

Moss AJ, Ryan D, Oakes D, Goldstein RE, Greenberg H, Bodenheimer MM, Brown MW, Case RB, Dwyer EM, Eberly SW, Francis CW, Gillespie JA, Krone RJ, Lichstein E, MacCluer JW, Marcus FI, McCarthy J, Sparks CE, Zareba W

The American journal of cardiology.. 2005 July 1596 (2):177-82. Epub 1900 01 01.

Novel compound heterozygous mutations in the KCNQ1 gene associated with autosomal recessive long QT syndrome (Jervell and Lange-Nielsen syndrome).

Ning L, Moss AJ, Zareba W, Robinson J, Rosero S, Ryan D, Qi M

Annals of noninvasive electrocardiology : the official journal of the International Society for Holter and Noninvasive Electrocardiology, Inc.. 2003 July 8 (3):246-50. Epub 1900 01 01.

Association of heterozygous hemochromatosis C282Y gene mutation with hand osteoarthritis.

Ross JM, Kowalchuk RM, Shaulinsky J, Ross L, Ryan D, Phatak PD

The Journal of rheumatology.. 2003 January 30 (1):121-5. Epub 1900 01 01.

Early gene activation in chronic leukemic B lymphocytes induced toward a plasma cell phenotype.

Segel GB, Woodlock TJ, Xu J, Li L, Felgar RE, Ryan DH, Lichtman MA, Wang N

Blood cells, molecules & diseases.. 2003 30 (3):277-87. Epub 1900 01 01.

Denaturing high-performance liquid chromatography quickly and reliably detects cardiac ion channel mutations in long QT syndrome.

Ning L, Moss A, Zareba W, Robinson J, Rosero S, Ryan D, Qi M

Genetic testing.. 2003 7 (3):249-53. Epub 1900 01 01.

Prevalence and penetrance of HFE mutations in 4865 unselected primary care patients.

Phatak PD, Ryan DH, Cappuccio J, Oakes D, Braggins C, Provenzano K, Eberly S, Sham RL

Blood cells, molecules & diseases.. 2002 29 (1):41-7. Epub 1900 01 01.

Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance.

Pugh-Bernard AE, Silverman GJ, Cappione AJ, Villano ME, Ryan DH, Insel RA, Sanz I

The Journal of clinical investigation.. 2001 October 108 (7):1061-70. Epub 1900 01 01.

Detection of a group of risk factors in coronary disease using a new carriership analysis approach.

Watelet LF, Moss AJ, Zareba W, Oakes D, Ryan D

The American journal of cardiology.. 2000 December 186 (11):1253-6, A6. Epub 1900 01 01.

Expression of cyclooxygenase-2 and prostaglandins by B-1 cells and B-CLL cells.

Phipps RP, Pollock SJ, Kaur K, Kaufman J, Borrello MA, Graf BA, Nazarenko D, Roberts LJ, Morrow JD, Palis J, Ryan DJ, Bennett JM

Current topics in microbiology and immunology.. 2000 252 :293-300. Epub 1900 01 01.

Treatment of chronic myelogenous leukemia with autologous bone marrow transplantation followed by roquinimex.

Rowe JM, Rapoport AP, Ryan DH, Nilsson BI, Duerst RE, Packman CH, Abboud CN, DiPersio JF, Linder T, Wang N, Simonsson B, Liesveld JL

Bone marrow transplantation.. 1999 November 24 (10):1057-63. Epub 1900 01 01.

Non-PCR-dependent detection of the factor V Leiden mutation from genomic DNA using a homogeneous invader microtiter plate assay.

Ryan D, Nuccie B, Arvan D

Molecular diagnosis : a journal devoted to the understanding of human

Relation of factor V Leiden genotype to risk for acute deep venous thrombosis after joint replacement surgery.

Ryan DH, Crowther MA, Ginsberg JS, Francis CW

Annals of internal medicine.. 1998 February 15128 (4):270-6. Epub 1900 01 01.

Telomerase is up-regulated in human germinal center B cells in vivo and can be re-expressed in memory B cells activated in vitro.

Hu BT, Lee SC, Marin E, Ryan DH, Insel RA

The Journal of immunology : official journal of the American Association of Immunologists.. 1997 August 1159 (3):1068-71. Epub 1900 01 01.

TGF-beta down-regulates stromal IL-7 secretion and inhibits proliferation of human B cell precursors.

Tang J, Nuccie BL, Ritterman I, Liesveld JL, Abboud CN, Ryan DH

The Journal of immunology : official journal of the American Association of Immunologists.. 1997 July 1159 (1):117-25. Epub 1900 01 01.

Expression of interleukin-7 receptor by lineage-negative human bone marrow progenitors with enhanced lymphoid proliferative potential and B-lineage differentiation capacity.

Ryan DH, Nuccie BL, Ritterman I, Liesveld JL, Abboud CN, Insel RA

Blood.. 1997 February 189 (3):929-40. Epub 1900 01 01.

Regulation of human B cell lymphopoiesis by adhesion molecules and cytokines.

Ryan DH, Tang J

Leukemia & lymphoma.. 1995 May 17 (5-6):375-89. Epub 1900 01 01.

CD34+ progenitor cell isolation from blood and marrow: a comparison of techniques for small-scale selection.

Winslow JM, Liesveld JL, Ryan DH, Dipersio JF, Abboud CN

Bone marrow transplantation.. 1994 August 14 (2):265-71. Epub 1900 01 01.

Cytokine regulation of early human lymphopoiesis.

Ryan DH, Nuccie BL, Ritterman I, Liesveld JL, Abboud CN

The Journal of immunology : official journal of the American Association of Immunologists.. 1994 June 1152 (11):5250-8. Epub 1900 01 01.

Stem cell factor regulates human melanocyte-matrix interactions.

Scott G, Ewing J, Ryan D, Abboud C

Pigment cell research. 1994 February 7 (1):44-51. Epub 1900 01 01.

Subpopulations of bone marrow fibroblasts support VLA-4-mediated migration of B-cell precursors.

Tang J, Scott G, Ryan DH

Blood.. 1993 December 182 (11):3415-23. Epub 1900 01 01.

Autografting in chronic myelogenous leukemia followed by immunotherapy.

Rowe J, Ryan D, Dipersio J, Gaspari A, Nilsson B, Larsson L, Liesveld J, Kouides P, Simonsson B

Stem cells.. 1993 October 11 Suppl 3 :34-42. Epub 1900 01 01.

Expression of integrins and examination of their adhesive function in normal and leukemic hematopoietic cells.

Liesveld JL, Winslow JM, Frediani KE, Ryan DH, Abboud CN

Blood.. 1993 January 181 (1):112-21. Epub 1900 01 01.

Adherence of normal and neoplastic human B cell precursors to the bone marrow microenvironment.

Ryan DH

Blood cells. 1993 19 (2):225-41; discussion 241-4. Epub 1900 01 01.

Inhibition of human bone marrow lymphoid progenitor colonies by antibodies to VLA integrins.

Ryan DH, Nuccie BL, Abboud CN

The Journal of immunology : official journal of the American Association of Immunologists.. 1992 December 1149 (11):3759-64. Epub 1900 01 01.

Molecular mechanisms of human melanocyte attachment to fibronectin.

Scott G, Ryan DH, McCarthy JB

The Journal of investigative dermatology.. 1992 December 99 (6):787-94. Epub 1900 01 01.

Phenotypic heterogeneity in acute leukemia.

Ryan DH

Clinica chimica acta; international journal of clinical chemistry.. 1992 March 13206 (1-2):9-23. Epub 1900 01 01.

A clinical and immunologic study of blood transfusion and postoperative bacterial infection in spinal surgery.

Triulzi DJ, Vanek K, Ryan DH, Blumberg N

Transfusion.. 1992 32 (6):517-24. Epub 1900 01 01.

Characterization of human alveolar macrophage Fc gamma receptor III: a transmembrane glycoprotein that is shed under in vitro culture conditions.

Levy PC, Utell MJ, Fleit HB, Roberts NJ, Ryan DH, Looney RJ

American journal of respiratory cell and molecular biology.. 1991 October 5 (4):307-14. Epub 1900 01 01.

Vascular cell adhesion molecule-1 and the integrin VLA-4 mediate adhesion of human B cell precursors to cultured bone marrow adherent cells.

Ryan DH, Nuccie BL, Abboud CN, Winslow JM

The Journal of clinical investigation.. 1991 September 88 (3):995-1004. Epub 1900 01 01.

Adhesive interactions of normal and leukemic human CD34+ myeloid progenitors: role of marrow stromal, fibroblast, and cytomatrix components.

Liesveld JL, Winslow JM, Kempski MC, Ryan DH, Brennan JK, Abboud CN

Experimental hematology.. 1991 January 19 (1):63-70. Epub 1900 01 01.

Maturation-dependent adhesion of human B cell precursors to the bone marrow microenvironment.

Ryan DH, Nuccie BL, Abboud CN, Liesveld JL

The Journal of immunology : official journal of the American Association of Immunologists.. 1990 July 15145 (2):477-84. Epub 1900 01 01.

The clinical course of multiple sclerosis during pregnancy and the puerperium.

Birk K, Ford C, Smeltzer S, Ryan D, Miller R, Rudick RA

Archives of neurology.. 1990 July 47 (7):738-42. Epub 1900 01 01.

Human alveolar macrophage FcR-mediated cytotoxicity. Heteroantibody- versus conventional antibody-mediated target cell lysis.

Levy PC, Looney RJ, Shen L, Graziano RF, Fanger MW, Roberts NJ, Ryan DH, Utell MJ

The Journal of immunology : official journal of the American Association of Immunologists.. 1990 May 15144 (10):3693-700. Epub 1900 01 01.

Rearrangement of the T-cell receptor alpha, beta and gamma chain genes in chronic lymphocytic leukemia.

Perl A, DiVincenzo JP, Ryan DH, Gergely P, Szigeti A, Feher J, Abraham GN

Leukemia research.. 1990 14 (2):131-7. Epub 1900 01 01.

In vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase.

Asselin BL, Ryan D, Frantz CN, Bernal SD, Leavitt P, Sallan SE, Cohen HJ

Cancer research.. 1989 August 149 (15):4363-8. Epub 1900 01 01.

Characterization of human marrow stromal cells: role in progenitor cell binding and granulopoiesis.

Liesveld JL, Abboud CN, Duerst RE, Ryan DH, Brennan JK, Lichtman MA

Blood.. 1989 May 1573 (7):1794-800. Epub 1900 01 01.

Clonal B cell expansions in patients with essential mixed cryoglobulinaemia.

Perl A, Gorevic PD, Ryan DH, Condemi JJ, Ruszkowski RJ, Abraham GN

Clinical and experimental immunology.. 1989 April 76 (1):54-60. Epub 1900 01 01.

Structural polymorphism of the human platelet Fc gamma receptor.

Looney RJ, Anderson CL, Ryan DH, Rosenfeld SI

The Journal of immunology : official journal of the American Association of Immunologists.. 1988 October 15141 (8):2680-3. Epub 1900 01 01.

Expression of IgG Fc receptors in myeloid leukemic cell lines. Effect of colony-stimulating factors and cytokines.

Liesveld JL, Abboud CN, Looney RJ, Ryan DH, Brennan JK

The Journal of immunology : official journal of the American Association of Immunologists.. 1988 March 1140 (5):1527-33. Epub 1900 01 01.

Flow cytometry in the clinical laboratory.

Ryan DH, Fallon MA, Horan PK

Clinica chimica acta; international journal of clinical chemistry.. 1988 February 15171 (2-3):125-73. Epub 1900 01 01.

Evaluation of acridine orange-stained buffy coat smears for identification of bacteremia in children.

Henrickson KJ, Powell KR, Ryan DH

The Journal of pediatrics.. 1988 January 112 (1):65-6. Epub 1900 01 01.

Sensitive detection of rare metastatic human neuroblastoma cells in bone marrow by two-color immunofluorescence and cell sorting.

Frantz CN, Ryan DH, Cheung NV, Duerst RE, Wilbur DC

Progress in clinical and biological research.. 1988 271 :249-62. Epub 1900 01 01.

Functional characterization of renal infiltrating cells following allograft nephrectomy.

Nikaein A, McQueen K, Boyer B, Landesberg R, Ryan DH, Insel RA

Transplantation proceedings.. 1987 February 19 (1 Pt 1):398-400. Epub 1900 01 01.