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Eric M. Phizicky, Ph.D.

Eric M. Phizicky, Ph.D.

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About Me

Faculty Appointments

Professor - Department of Biochemistry and Biophysics (SMD)

Credentials

Education

PhD | Cornell University. Biochemistry. 1983

BS | McGill University. Biochemistry. 1976

Research

tRNA Biogenesis, Function and Quality Control

One major lab project focuses on understanding the biology of tRNA. tRNA plays a crucial role in translation, and numerous factors participate in its biogenesis, function, intracellular trafficking, and quality control. In the yeast Saccharomyces ...
tRNA Biogenesis, Function and Quality Control

One major lab project focuses on understanding the biology of tRNA. tRNA plays a crucial role in translation, and numerous factors participate in its biogenesis, function, intracellular trafficking, and quality control. In the yeast Saccharomyces cerevisiae, cytoplasmic tRNAs have an average of 13 modifications, and there are a total of 25 chemically distinct modifications, many of which are highly conserved in different organisms, and of unknown function. We previously used a biochemical genomics approach to identify the genes encoding a number of different modification enzymes. We are currently focusing on the assignment of other activities to genes, and on two aspects of the biology of modifications: First, we are investigating the roles of modifications. We recently found evidence that lack of certain modifications can lead to rapid degradation of specific mature tRNAs, suggesting the existence of a quality control pathway that monitors the integrity of tRNA. We are currently working to define the components of this rapid tRNA decay pathway, the mechanism by which tRNAs are targeted for turnover, and the conditions under which this pathway is used. Second, we are defining the mechanisms by which modification enzymes recognize and act on their specific tRNA substrates. Since modifications have crucial roles in the cell, incorrect modification of a tRNA is likely to have catastrophic effects. We recently found that recognition of the tRNAHis anticodon is necessary and sufficient for Thg1 to add a guanine nucleotide to the 5' end of the tRNA, a reaction that is essential in wild type cells for proper translation. We are currently examining other modification enzymes to uncover the different mechanisms by which specificity is attained.

Functional Genomics

A second major lab project focuses on the design, construction and implementation of genomic methods to analyze protein structure and function (in collaboration with Elizabeth Grayhack). To facilitate parallel biochemical analysis of the proteome, we previously constructed two genomic libraries of strains in which nearly every yeast gene is expressed as a tagged fusion protein under regulated control. We are currently using these libraries to define new biochemical activities, and to help define cell circuitry by systematic analysis of genetic suppression caused by overexpression of genes. In addition, in collaboration with investigators at the Hauptman-Woodward Institute, we are developing methodology for high throughput expression and purification of protein complexes from yeast, for structural analysis by x-ray crystallography.

Publications

Journal Articles

A connection between the ribosome and two S. pombe tRNA modification mutants subject to rapid tRNA decay.

De Zoysa T, Hauke AC, Iyer NR, Marcus E, Ostrowski SM, Stegemann F, Ermolenko DN, Fay JC, Phizicky EM

PLoS genetics.. 2024 January 20 (1):e1011146. Epub 01/31/2024.

A connection between the ribosome and two tRNA modification mutants subject to rapid tRNA decay.

De Zoysa T, Hauke AC, Iyer NR, Marcus E, Ostrowski SM, Fay JC, Phizicky EM

bioRxiv : the preprint server for biology.. 2023 September 18 Epub 09/18/2023.

The life and times of a tRNA.

Phizicky EM, Hopper AK

RNA.. 2023 July 29 (7):898-957. Epub 04/13/2023.

Initiator tRNA lacking 1-methyladenosine is targeted by the rapid tRNA decay pathway in evolutionarily distant yeast species.

Tasak M, Phizicky EM

PLoS genetics.. 2022 July 18 (7):e1010215. Epub 07/28/2022.

Identification of a Trm732 Motif Required for 2'--methylation of the tRNA Anticodon Loop by Trm7.

Funk HM, DiVita DJ, Sizemore HE, Wehrle K, Miller CLW, Fraley ME, Mullins AK, Guy AR, Phizicky EM, Guy MP

ACS omega.. 2022 April 267 (16):13667-13675. Epub 04/13/2022.

A semi-quantitative pull-down assay to study tRNA substrate specificity of modification enzymes.

Han L, Marcus E, Phizicky EM

Methods in enzymology.. 2021 658 :359-377. Epub 07/14/2021.

Hypomodified tRNA in evolutionarily distant yeasts can trigger rapid tRNA decay to activate the general amino acid control response, but with different consequences.

De Zoysa T, Phizicky EM

PLoS genetics.. 2020 August 16 (8):e1008893. Epub 08/25/2020.

Mutations in the anticodon stem of tRNA cause accumulation and Met22-dependent decay of pre-tRNA in yeast.

Payea MJ, Hauke AC, De Zoysa T, Phizicky EM

RNA.. 2020 January 26 (1):29-43. Epub 10/16/2019.

Formation of tRNA Wobble Inosine in Humans Is Disrupted by a Millennia-Old Mutation Causing Intellectual Disability.

Ramos J, Han L, Li Y, Hagelskamp F, Kellner SM, Alkuraya FS, Phizicky EM, Fu D

Molecular and cellular biology.. 2019 October 139 (19)Epub 09/11/2019.

PUS7 mutations impair pseudouridylation in humans and cause intellectual disability and microcephaly.

Shaheen R, Tasak M, Maddirevula S, Abdel-Salam GMH, Sayed ISM, Alazami AM, Al-Sheddi T, Alobeid E, Phizicky EM, Alkuraya FS

Human genetics.. 2019 March 138 (3):231-239. Epub 02/18/2019.

A rationale for tRNA modification circuits in the anticodon loop.

Han L, Phizicky EM

RNA.. 2018 October 24 (10):1277-1284. Epub 07/19/2018.

Conditional accumulation of toxic tRNAs to cause amino acid misincorporation.

Zimmerman SM, Kon Y, Hauke AC, Ruiz BY, Fields S, Phizicky EM

Nucleic acids research.. 2018 September 646 (15):7831-7843. Epub 1900 01 01.

Widespread temperature sensitivity and tRNA decay due to mutations in a yeast tRNA.

Payea MJ, Sloma MF, Kon Y, Young DL, Guy MP, Zhang X, De Zoysa T, Fields S, Mathews DH, Phizicky EM

RNA.. 2018 March 24 (3):410-422. Epub 12/19/2017.

Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.

Han L, Guy MP, Kon Y, Phizicky EM

PLoS genetics.. 2018 March 14 (3):e1007288. Epub 03/29/2018.

Trm140 has two recognition modes for 3-methylcytidine modification of the anticodon loop of tRNA substrates.

Han L, Marcus E, D'Silva S, Phizicky EM

RNA.. 2017 March 23 (3):406-419. Epub 12/21/2016.

A genome wide dosage suppressor network reveals genomic robustness.

Patra B, Kon Y, Yadav G, Sevold AW, Frumkin JP, Vallabhajosyula RR, Hintze A, Østman B, Schossau J, Bhan A, Marzolf B, Tamashiro JK, Kaur A, Baliga NS, Grayhack EJ, Adami C, Galas DJ, Raval A, Phizicky EM, Ray A

Nucleic acids research.. 2017 January 945 (1):255-270. Epub 11/29/2016.

High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq).

Hrabeta-Robinson E, Marcus E, Cozen AE, Phizicky EM, Lowe TM

Methods in molecular biology.. 2017 1562 :231-243. Epub 1900 01 01.

A homozygous truncating mutation in PUS3 expands the role of tRNA modification in normal cognition.

Shaheen R, Han L, Faqeih E, Ewida N, Alobeid E, Phizicky EM, Alkuraya FS

Human genetics.. 2016 July 135 (7):707-13. Epub 04/07/2016.

Defects in tRNA Anticodon Loop 2'-O-Methylation Are Implicated in Nonsyndromic X-Linked Intellectual Disability due to Mutations in FTSJ1.

Guy MP, Shaw M, Weiner CL, Hobson L, Stark Z, Rose K, Kalscheuer VM, Gecz J, Phizicky EM

Human mutation.. 2015 December 36 (12):1176-87. Epub 09/10/2015.

Mutation in WDR4 impairs tRNA m(7)G46 methylation and causes a distinct form of microcephalic primordial dwarfism.

Shaheen R, Abdel-Salam GM, Guy MP, Alomar R, Abdel-Hamid MS, Afifi HH, Ismail SI, Emam BA, Phizicky EM, Alkuraya FS

Genome biology.. 2015 September 2816 :210. Epub 09/28/2015.

ARM-seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments.

Cozen AE, Quartley E, Holmes AD, Hrabeta-Robinson E, Phizicky EM, Lowe TM

Nature methods.. 2015 September 12 (9):879-84. Epub 08/03/2015.

tRNA processing, modification, and subcellular dynamics: past, present, and future.

Phizicky EM, Hopper AK

RNA.. 2015 April 21 (4):483-5. Epub 1900 01 01.

Functional importance of ?38 and ?39 in distinct tRNAs, amplified for tRNAGln(UUG) by unexpected temperature sensitivity of the s2U modification in yeast.

Han L, Kon Y, Phizicky EM

RNA.. 2015 February 21 (2):188-201. Epub 12/12/2014.

Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes.

Guy MP, Phizicky EM

RNA.. 2015 January 21 (1):61-74. Epub 11/17/2014.

Methodology for the High-Throughput Identification and Characterization of tRNA Variants That Are Substrates for a tRNA Decay Pathway.

Payea MJ, Guy MP, Phizicky EM

Methods in enzymology.. 2015 560 :1-17. Epub 04/27/2015.

Causal signals between codon bias, mRNA structure, and the efficiency of translation and elongation.

Pop C, Rouskin S, Ingolia NT, Han L, Phizicky EM, Weissman JS, Koller D

Molecular systems biology.. 2014 December 2310 (12):770. Epub 12/23/2014.

Influence of Sequence and Covalent Modifications on Yeast tRNA Dynamics.

Zhang X, Walker RC, Phizicky EM, Mathews DH

Journal of chemical theory and computation.. 2014 August 1210 (8):3473-3483. Epub 05/28/2014.

Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.

Guy MP, Young DL, Payea MJ, Zhang X, Kon Y, Dean KM, Grayhack EJ, Mathews DH, Fields S, Phizicky EM

Genes & development.. 2014 August 128 (15):1721-32. Epub 1900 01 01.

Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.

Guy MP, Phizicky EM

RNA biology.. 2014 11 (12):1608-18. Epub 1900 01 01.

tRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae.

Preston MA, D'Silva S, Kon Y, Phizicky EM

RNA.. 2013 February 19 (2):243-56. Epub 12/18/2012.

The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications.

Dewe JM, Whipple JM, Chernyakov I, Jaramillo LN, Phizicky EM

RNA.. 2012 October 18 (10):1886-96. Epub 08/15/2012.

Yeast Trm7 interacts with distinct proteins for critical modifications of the tRNAPhe anticodon loop.

Guy MP, Podyma BM, Preston MA, Shaheen HH, Krivos KL, Limbach PA, Hopper AK, Phizicky EM

RNA.. 2012 October 18 (10):1921-33. Epub 08/21/2012.

Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.

Grant TD, Snell EH, Luft JR, Quartley E, Corretore S, Wolfley JR, Elizabeth Snell M, Hadd A, Perona JJ, Phizicky EM, Grayhack EJ

Nucleic acids research.. 2012 April 40 (8):3723-31. Epub 12/17/2011.

The yeast rapid tRNA decay pathway primarily monitors the structural integrity of the acceptor and T-stems of mature tRNA.

Whipple JM, Lane EA, Chernyakov I, D'Silva S, Phizicky EM

Genes & development.. 2011 June 125 (11):1173-84. Epub 1900 01 01.

A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop.

D'Silva S, Haider SJ, Phizicky EM

RNA.. 2011 June 17 (6):1100-10. Epub 04/25/2011.

tRNA biology charges to the front.

Phizicky EM, Hopper AK

Genes & development.. 2010 September 124 (17):1832-60. Epub 1900 01 01.

The requirement for the highly conserved G-1 residue of Saccharomyces cerevisiae tRNAHis can be circumvented by overexpression of tRNAHis and its synthetase.

Preston MA, Phizicky EM

RNA.. 2010 May 16 (5):1068-77. Epub 04/01/2010.

Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae.

Murthi A, Shaheen HH, Huang HY, Preston MA, Lai TP, Phizicky EM, Hopper AK

Molecular biology of the cell.. 2010 February 1521 (4):639-49. Epub 12/23/2009.

Do all modifications benefit all tRNAs?

Phizicky EM, Alfonzo JD

FEBS letters.. 2010 January 21584 (2):265-71. Epub 1900 01 01.

Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.

Quartley E, Alexandrov A, Mikucki M, Buckner FS, Hol WG, Detitta GT, Phizicky EM, Grayhack EJ

Journal of structural and functional genomics. 2009 September 10 (3):233-47. Epub 08/22/2009.

Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.

Chernyakov I, Whipple JM, Kotelawala L, Grayhack EJ, Phizicky EM

Genes & development.. 2008 May 1522 (10):1369-80. Epub 04/28/2008.

Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase.

Jackman JE, Phizicky EM

Biochemistry.. 2008 April 2247 (16):4817-25. Epub 03/27/2008.

Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.

Kotelawala L, Grayhack EJ, Phizicky EM

RNA.. 2008 January 14 (1):158-69. Epub 11/19/2007.

Waste not, want not: a case for tRNA repair

Phizicky, E. M.;.

Nat Struct Mol Biol. 2008; 15(38): 783-4.

Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function

Merritt, E. A.; Holmes, M.; Buckner, F. S.; Van Voorhis, W. C.; Quartly, E.; Phizicky, E. M.; Lauricella, A.; Luft, J.; DeTitta, G.; Neely, H.; Zucker, F.; Hol, W. G.;.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008; 64(3Pt 6): 474-8.

The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.

Jackman JE, Grayhack EJ, Phizicky EM

Methods in molecular biology.. 2008 488 :383-93. Epub 1900 01 01.

Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.

Chernyakov I, Baker MA, Grayhack EJ, Phizicky EM

Methods in enzymology.. 2008 449 :221-37. Epub 1900 01 01.

Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.

Malkowski MG, Quartley E, Friedman AE, Babulski J, Kon Y, Wolfley J, Said M, Luft JR, Phizicky EM, DeTitta GT, Grayhack EJ

Proceedings of the National Academy of Sciences of the United States of America.. 2007 April 17104 (16):6678-83. Epub 04/10/2007.

The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.

Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM

RNA.. 2007 March 13 (3):404-13. Epub 01/22/2007.

Identification and characterization of modification enzymes by biochemical analysis of the proteome.

Jackman JE, Kotelawala L, Grayhack EJ, Phizicky EM

Methods in enzymology.. 2007 425 :139-52. Epub 1900 01 01.

tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition.

Jackman JE, Phizicky EM

Proceedings of the National Academy of Sciences of the United States of America.. 2006 June 6103 (23):8640-5. Epub 05/26/2006.

tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases.

Jackman JE, Phizicky EM

RNA.. 2006 June 12 (6):1007-14. Epub 04/19/2006.

Rapid tRNA decay can result from lack of nonessential modifications.

Alexandrov A, Chernyakov I, Gu W, Hiley SL, Hughes TR, Grayhack EJ, Phizicky EM

Molecular cell.. 2006 January 621 (1):87-96. Epub 1900 01 01.

Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major

Arakaki, T.; Le Trong, I.; Phizicky, E. M.; Quartley, E.; DeTitta, G.; Luft, J.; Lauricella, A.; Anderson, L.; Kalyuzhniy, O.; Worthey, E.; Myler, P. J.; Kim, D.; Baker, D.; Hol, W. G.; Merritt, E. A.;.

Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006; 62(Pt 3)(3): 175-179.

Proteome-scale analysis of biochemical activity.

Phizicky EM, Grayhack EJ

Critical reviews in biochemistry and molecular biology. 2006 41 (5):315-27. Epub 1900 01 01.

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.

Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky EM, Snyder M, Grayhack EJ

Genes & development.. 2005 December 119 (23):2816-26. Epub 1900 01 01.

Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.

Gu W, Hurto RL, Hopper AK, Grayhack EJ, Phizicky EM

Molecular and cellular biology.. 2005 September 25 (18):8191-201. Epub 1900 01 01.

Have tRNA, will travel.

Phizicky EM

Proceedings of the National Academy of Sciences of the United States of America.. 2005 August 9102 (32):11127-8. Epub 08/01/2005.

The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells.

Cartlidge RA, Knebel A, Peggie M, Alexandrov A, Phizicky EM, Cohen P

The EMBO journal.. 2005 May 424 (9):1696-705. Epub 04/14/2005.

tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.

Alexandrov A, Grayhack EJ, Phizicky EM

RNA.. 2005 May 11 (5):821-30. Epub 04/05/2005.

Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.

Steiger MA, Jackman JE, Phizicky EM

RNA.. 2005 January 11 (1):99-106. Epub 1900 01 01.

A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae.

Shull NP, Spinelli SL, Phizicky EM

Nucleic acids research.. 2005 33 (2):650-60. Epub 01/31/2005.

A facile method for high-throughput co-expression of protein pairs.

Alexandrov A, Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, Babulski J, Mitchell SF, Schoenfeld LW, Fields S, Hol WG, Dumont ME, Phizicky EM, Grayhack EJ

Molecular & cellular proteomics : MCP.. 2004 September 3 (9):934-8. Epub 07/07/2004.

The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs.

Xing F, Hiley SL, Hughes TR, Phizicky EM

The Journal of biological chemistry.. 2004 April 23279 (17):17850-60. Epub 02/16/2004.

tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis.

Gu W, Jackman JE, Lohan AJ, Gray MW, Phizicky EM

Genes & development.. 2003 December 117 (23):2889-901. Epub 11/21/2003.

Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9.

Jackman JE, Montange RK, Malik HS, Phizicky EM

RNA.. 2003 May 9 (5):574-85. Epub 1900 01 01.

Protein analysis on a proteomic scale.

Phizicky E, Bastiaens PI, Zhu H, Snyder M, Fields S

Nature.. 2003 March 13422 (6928):208-15. Epub 1900 01 01.

Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA.

Alexandrov A, Martzen MR, Phizicky EM

RNA.. 2002 October 8 (10):1253-66. Epub 1900 01 01.

A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA.

Xing F, Martzen MR, Phizicky EM

RNA.. 2002 March 8 (3):370-81. Epub 1900 01 01.

Biochemical genomics approach to map activities to genes.

Phizicky EM, Martzen MR, McCraith SM, Spinelli SL, Xing F, Shull NP, Van Slyke C, Montagne RK, Torres FM, Fields S, Grayhack EJ

Methods in enzymology.. 2002 350 :546-59. Epub 1900 01 01.

Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog.

Steiger MA, Kierzek R, Turner DH, Phizicky EM

Biochemistry.. 2001 November 2040 (46):14098-105. Epub 1900 01 01.

Genomic analysis of biochemical function.

Grayhack EJ, Phizicky EM

Current opinion in chemical biology.. 2001 February 5 (1):34-9. Epub 1900 01 01.

Cytochrome c methyltransferase, Ctm1p, of yeast.

Polevoda B, Martzen MR, Das B, Phizicky EM, Sherman F

The Journal of biological chemistry.. 2000 July 7275 (27):20508-13. Epub 1900 01 01.

The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates.

Kierzek R, Steiger MA, Spinelli SL, Turner DH, Phizicky EM

Nucleosides, nucleotides & nucleic acids.. 2000 19 (5-6):917-33. Epub 1900 01 01.

A biochemical genomics approach for identifying genes by the activity of their products.

Martzen MR, McCraith SM, Spinelli SL, Torres FM, Fields S, Grayhack EJ, Phizicky EM

Science.. 1999 November 5286 (5442):1153-5. Epub 1900 01 01.

Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast.

Spinelli SL, Kierzek R, Turner DH, Phizicky EM

The Journal of biological chemistry.. 1999 January 29274 (5):2637-44. Epub 1900 01 01.

A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.

Spinelli SL, Malik HS, Consaul SA, Phizicky EM

Proceedings of the National Academy of Sciences of the United States of America.. 1998 November 2495 (24):14136-41. Epub 1900 01 01.

Purification and assay of the Ptc1/Tpd1 protein phosphatase 2C from the yeast Saccharomyces cerevisiae.

Robinson MK, Phizicky EM

Methods in molecular biology.. 1998 93 :235-42. Epub 1900 01 01.

A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction.

Spinelli SL, Consaul SA, Phizicky EM

RNA.. 1997 December 3 (12):1388-400. Epub 1900 01 01.

A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae.

Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM

The Journal of biological chemistry.. 1997 May 16272 (20):13203-10. Epub 1900 01 01.

Protein-protein interactions: methods for detection and analysis.

Phizicky EM, Fields S

Microbiological reviews. 1995 March 59 (1):94-123. Epub 1900 01 01.

tRNA splicing in yeast and wheat germ. A cyclic phosphodiesterase implicated in the metabolism of ADP-ribose 1",2"-cyclic phosphate.

Culver GM, Consaul SA, Tycowski KT, Filipowicz W, Phizicky EM

The Journal of biological chemistry.. 1994 October 7269 (40):24928-34. Epub 1900 01 01.

TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation.

Robinson MK, van Zyl WH, Phizicky EM, Broach JR

Molecular and cellular biology.. 1994 June 14 (6):3634-45. Epub 1900 01 01.

An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate.

Culver GM, McCraith SM, Zillmann M, Kierzek R, Michaud N, LaReau RD, Turner DH, Phizicky EM

Science.. 1993 July 9261 (5118):206-8. Epub 1900 01 01.

Pre-tRNA splicing: variation on a theme or exception to the rule?

Phizicky EM, Greer CL

Trends in biochemical sciences.. 1993 January 18 (1):31-4. Epub 1900 01 01.

HeLa cells contain a 2'-phosphate-specific phosphotransferase similar to a yeast enzyme implicated in tRNA splicing.

Zillmann M, Gorovsky MA, Phizicky EM

The Journal of biological chemistry.. 1992 May 25267 (15):10289-94. Epub 1900 01 01.

Yeast tRNA ligase mutants are nonviable and accumulate tRNA splicing intermediates.

Phizicky EM, Consaul SA, Nehrke KW, Abelson J

The Journal of biological chemistry.. 1992 March 5267 (7):4577-82. Epub 1900 01 01.

Conserved mechanism of tRNA splicing in eukaryotes.

Zillmann M, Gorovsky MA, Phizicky EM

Molecular and cellular biology.. 1991 November 11 (11):5410-6. Epub 1900 01 01.

An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.

McCraith SM, Phizicky EM

The Journal of biological chemistry.. 1991 June 25266 (18):11986-92. Epub 1900 01 01.

A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.

McCraith SM, Phizicky EM

Molecular and cellular biology.. 1990 March 10 (3):1049-55. Epub 1900 01 01.

Purification of yeast transfer RNA ligase.

Xu Q, Phizicky EM, Greer CL, Abelson JN

Methods in enzymology.. 1990 181 :463-71. Epub 1900 01 01.

Structure and function of the yeast tRNA ligase gene.

Westaway SK, Phizicky EM, Abelson J

The Journal of biological chemistry.. 1988 March 5263 (7):3171-6. Epub 1900 01 01.

Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene.

Phizicky EM, Schwartz RC, Abelson J

The Journal of biological chemistry.. 1986 February 25261 (6):2978-86. Epub 1900 01 01.

Induction of SOS functions: regulation of proteolytic activity of E. coli RecA protein by interaction with DNA and nucleoside triphosphate.

Phizicky EM, Roberts JW

Cell.. 1981 July 25 (1):259-67. Epub 1900 01 01.

Kinetics of RecA protein-directed inactivation of repressors of phage lambda and phage P22.

Phizicky EM, Roberts JW

Journal of molecular biology.. 1980 May 25139 (3):319-28. Epub 1900 01 01.

Activity of the Escherichia coli recA-gene product.

Roberts JW, Roberts CW, Craig NL, Phizicky EM

Cold Spring Harbor symposia on quantitative biology.. 1979 43 Pt 2 :917-20. Epub 1900 01 01.