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Mark E. Dumont, Ph.D.

Mark E. Dumont, Ph.D.

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About Me

Faculty Appointments

Professor - Department of Biochemistry and Biophysics (SMD)

Credentials

Education

PhD | Johns Hopkins University. Biophysics. 1980

MS | Western Washington State Coll. Physics. 1975

BA | Harvard University. Physics. 1972

Research

Transmembrane proteins carry out critical functions in transporting ions and metabolites in an out of cells and organelles, in transducing extracellular signals into intracellular responses, in cell-cell recognition, and in catalyzing a variety of enzymatic reactions. They also constitute 20-30% of ...
Transmembrane proteins carry out critical functions in transporting ions and metabolites in an out of cells and organelles, in transducing extracellular signals into intracellular responses, in cell-cell recognition, and in catalyzing a variety of enzymatic reactions. They also constitute 20-30% of the protein coding potential of most genomes. However, the structures and molecular mechanisms of the function of membrane proteins remain much more poorly characterized than is the case for most soluble proteins. The Dumont laboratory focuses on understanding mechanisms of membrane protein function at the molecular level.

Publications

Journal Articles

Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast ?-Factor Receptor.

Dumont ME, Konopka JB

Biomolecules.. 2022 May 3012 (6)Epub 05/30/2022.

Oligomerization of yeast ?-factor receptor detected by fluorescent energy transfer between ligands.

Connelly SM, Sridharan R, Naider F, Dumont ME

Biophysical journal.. 2021 November 16120 (22):5090-5106. Epub 10/08/2021.

Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies.

Zhu H, Mathew E, Connelly SM, Zuber J, Sullivan M, Piepenbrink MS, Kobie JJ, Dumont ME

PloS one.. 2019 14 (9):e0221550. Epub 09/10/2019.

Display of the HIV envelope protein at the yeast cell surface for immunogen development.

Mathew E, Zhu H, Connelly SM, Sullivan MA, Brewer MG, Piepenbrink MS, Kobie JJ, Dewhurst S, Dumont ME

PloS one.. 2018 13 (10):e0205756. Epub 10/18/2018.

Human CaaX protease ZMPSTE24 expressed in yeast: Structure and inhibition by HIV protease inhibitors.

Clark KM, Jenkins JL, Fedoriw N, Dumont ME

Protein science : a publication of the Protein Society.. 2017 February 26 (2):242-257. Epub 11/11/2016.

Structure of the SLC4 transporter Bor1p in an inward-facing conformation.

Coudray N, L Seyler S, Lasala R, Zhang Z, Clark KM, Dumont ME, Rohou A, Beckstein O, Stokes DL

Protein science : a publication of the Protein Society.. 2017 January 26 (1):130-145. Epub 10/21/2016.

Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.

Sridharan R, Connelly SM, Naider F, Dumont ME

The Journal of biological chemistry.. 2016 November 11291 (46):24261-24279. Epub 09/19/2016.

Deducing the symmetry of helical assemblies: Applications to membrane proteins.

Coudray N, Lasala R, Zhang Z, Clark KM, Dumont ME, Stokes DL

Journal of structural biology.. 2016 August 195 (2):167-178. Epub 05/30/2016.

The Crystal Structure of an Integral Membrane Fatty Acid ?-Hydroxylase.

Zhu G, Koszelak-Rosenblum M, Connelly SM, Dumont ME, Malkowski MG

The Journal of biological chemistry.. 2015 December 11290 (50):29820-33. Epub 10/28/2015.

Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.

Zuber J, Danial SA, Connelly SM, Naider FR, Dumont ME

Biochemistry.. 2015 March 1054 (9):1787-806. Epub 02/24/2015.

A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs.

Mathew E, Dumont ME

Methods in enzymology.. 2015 557 :27-43. Epub 03/24/2015.

Guided reconstitution of membrane protein fragments.

Cohen LS, Arshava B, Kauffman S, Mathew E, Fracchiolla KE, Ding FX, Dumont ME, Becker JM, Naider F

Biopolymers.. 2014 January 102 (1):16-29. Epub 1900 01 01.

Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors.

Sridharan R, Zuber J, Connelly SM, Mathew E, Dumont ME

Biochimica et biophysica acta.. 2014 January 1838 (1 Pt A):15-33. Epub 09/18/2013.

Structure of the integral membrane protein CAAX protease Ste24p.

Pryor EE, Horanyi PS, Clark KM, Fedoriw N, Connelly SM, Koszelak-Rosenblum M, Zhu G, Malkowski MG, Wiener MC, Dumont ME

Science.. 2013 March 29339 (6127):1600-4. Epub 1900 01 01.

Coordinating the impact of structural genomics on the human ?-helical transmembrane proteome.

Pieper U, Schlessinger A, Kloppmann E, Chang GA, Chou JJ, Dumont ME, Fox BG, Fromme P, Hendrickson WA, Malkowski MG, Rees DC, Stokes DL, Stowell MH, Wiener MC, Rost B, Stroud RM, Stevens RC, Sali A

Nature structural & molecular biology.. 2013 February 20 (2):135-8. Epub 1900 01 01.

Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.

Mathew E, Ding FX, Naider F, Dumont ME

Protein engineering, design & selection : PEDS.. 2013 January 26 (1):59-71. Epub 10/16/2012.

Functional and physical interactions among Saccharomyces cerevisiae ?-factor receptors.

Gehret AU, Connelly SM, Dumont ME

Eukaryotic cell.. 2012 October 11 (10):1276-88. Epub 08/24/2012.

Nanochannel-based single molecule recycling.

Lesoine JF, Venkataraman PA, Maloney PC, Dumont ME, Novotny L

Nano letters.. 2012 June 1312 (6):3273-8. Epub 06/04/2012.

Identifying functionally important conformational changes in proteins: activation of the yeast ?-factor receptor Ste2p.

Taslimi A, Mathew E, Celi? A, Wessel S, Dumont ME

Journal of molecular biology.. 2012 May 18418 (5):367-78. Epub 03/03/2012.

Oligomeric state of the oxalate transporter, OxlT.

Kang DC, Venkataraman PA, Dumont ME, Maloney PC

Biochemistry.. 2011 October 450 (39):8445-53. Epub 09/08/2011.

Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.

Mathew E, Bajaj A, Connelly SM, Sargsyan H, Ding FX, Hajduczok AG, Naider F, Dumont ME

Journal of molecular biology.. 2011 June 17409 (4):513-28. Epub 04/06/2011.

Binding of fluorinated phenylalanine alpha-factor analogues to Ste2p: evidence for a cation-pi binding interaction between a peptide ligand and its cognate G protein-coupled receptor.

Tantry SJ, Ding FX, Dumont ME, Becker JM, Naider FR

Biochemistry.. 2010 June 2249 (24):5007-15. Epub 1900 01 01.

Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.

Clark KM, Fedoriw N, Robinson K, Connelly SM, Randles J, Malkowski MG, Detitta GT, Dumont ME

Protein expression and purification.. 2010 June 71 (2):207-23. Epub 01/04/2010.

Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast.

Shi S, Notenboom S, Dumont ME, Ballatori N

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology.. 2010 25 (2-3):293-306. Epub 01/12/2010.

Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity.

Chloupková M, Pickert A, Lee JY, Souza S, Trinh YT, Connelly SM, Dumont ME, Dean M, Urbatsch IL

Biochemistry.. 2007 July 1046 (27):7992-8003. Epub 06/15/2007.

Role of extracellular charged amino acids in the yeast alpha-factor receptor.

Bajaj A, Connelly SM, Gehret AU, Naider F, Dumont ME

Biochimica et biophysica acta.. 2007 June 1773 (6):707-17. Epub 02/17/2007.

Characteristics affecting expression and solubilization of yeast membrane proteins.

White MA, Clark KM, Grayhack EJ, Dumont ME

Journal of molecular biology.. 2007 January 19365 (3):621-36. Epub 10/06/2006.

Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8.

Wang Z, Stalcup LD, Harvey BJ, Weber J, Chloupkova M, Dumont ME, Dean M, Urbatsch IL

Biochemistry.. 2006 August 1545 (32):9929-39. Epub 1900 01 01.

Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors.

Gehret AU, Bajaj A, Naider F, Dumont ME

The Journal of biological chemistry.. 2006 July 28281 (30):20698-20714. Epub 05/18/2006.

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.

Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky EM, Snyder M, Grayhack EJ

Genes & development.. 2005 December 119 (23):2816-26. Epub 1900 01 01.

A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast.

Bajaj A, Celi? A, Ding FX, Naider F, Becker JM, Dumont ME

Biochemistry.. 2004 October 2643 (42):13564-78. Epub 1900 01 01.

A facile method for high-throughput co-expression of protein pairs.

Alexandrov A, Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, Babulski J, Mitchell SF, Schoenfeld LW, Fields S, Hol WG, Dumont ME, Phizicky EM, Grayhack EJ

Molecular & cellular proteomics : MCP.. 2004 September 3 (9):934-8. Epub 07/07/2004.

Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors.

Roginskaya M, Connelly SM, Kim KS, Patel D, Dumont ME

Molecular genetics and genomics : MGG.. 2004 March 271 (2):237-48. Epub 02/07/2004.

Intensive mutational analysis of G protein-coupled receptors in yeast.

Celi? A, Connelly SM, Martin NP, Dumont ME

Methods in molecular biology.. 2004 237 :105-20. Epub 1900 01 01.

Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation.

Celi? A, Martin NP, Son CD, Becker JM, Naider F, Dumont ME

Biochemistry.. 2003 March 1842 (10):3004-17. Epub 1900 01 01.

Effects of Inserting Intracellular Loops of the Yeast a-factor Receptor, Ste3p, into the a-factor Receptor, Ste2p

Connelly, S.M.; Yuan, J.; Ding, F.X.; Naider, F.; Becker, J.M.; Dumont, M.E. ;.

Yeast. 2003; : in press.

Biosynthesis and Biophysical Analysis of Domains of a Yeast G protein-coupled receptor

Arevalo, E.; Estephan, R.; Madeo, J.; Arshava, B.; Dumont, M.E.; Becker, J.M.; Naider, F.;.

Biopolymers (Peptide Science). 2003; : in press.

Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor.

Martin NP, Celi? A, Dumont ME

Journal of molecular biology.. 2002 April 12317 (5):765-88. Epub 1900 01 01.

Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis.

Erlenbach I, Kostenis E, Schmidt C, Serradeil-Le Gal C, Raufaste D, Dumont ME, Pausch MH, Wess J

The Journal of biological chemistry.. 2001 August 3276 (31):29382-92. Epub 05/25/2001.

A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor.

Sommers CM, Martin NP, Akal-Strader A, Becker JM, Naider F, Dumont ME

Biochemistry.. 2000 June 1339 (23):6898-909. Epub 1900 01 01.

Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae.

Leavitt LM, Macaluso CR, Kim KS, Martin NP, Dumont ME

Molecular & general genetics : MGG.. 1999 July 261 (6):917-32. Epub 1900 01 01.

Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments.

Martin NP, Leavitt LM, Sommers CM, Dumont ME

Biochemistry.. 1999 January 1238 (2):682-95. Epub 1900 01 01.

Genetic Approaches for Studying the Structure and Function of G Protein-Coupled receptors in Yeast in Structure-Function Analysis of G Protein-Coupled Receptors

Sommers, C.M.; Dumont, M.E.;.

( J. Wess, ed.) Vol. III of Receptor Biochemistry and Methodology, , John Wiley and Sons, New York. 1999; : 141.

ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins.

Rebbeor JF, Connolly GC, Dumont ME, Ballatori N

The Journal of biological chemistry.. 1998 December 11273 (50):33449-54. Epub 1900 01 01.

ATP-dependent transport of reduced glutathione in yeast secretory vesicles.

Rebbeor JF, Connolly GC, Dumont ME, Ballatori N

The Biochemical journal.. 1998 September 15334 ( Pt 3) (Pt 3):723-9. Epub 1900 01 01.

Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition.

Pollock WB, Rosell FI, Twitchett MB, Dumont ME, Mauk AG

Biochemistry.. 1998 April 2837 (17):6124-31. Epub 1900 01 01.

Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene.

Sommers CM, Dumont ME

Journal of molecular biology.. 1997 February 28266 (3):559-75. Epub 1900 01 01.

Sequence requirements for mitochondrial import of yeast cytochrome c.

Wang X, Dumont ME, Sherman F

The Journal of biological chemistry.. 1996 March 22271 (12):6594-604. Epub 1900 01 01.

Noncovalent binding of heme induces a compact apocytochrome c structure.

Dumont ME, Corin AF, Campbell GA

Biochemistry.. 1994 June 1433 (23):7368-78. Epub 1900 01 01.

CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c.

Dumont ME, Schlichter JB, Cardillo TS, Hayes MK, Bethlendy G, Sherman F

Molecular and cellular biology.. 1993 October 13 (10):6442-51. Epub 1900 01 01.

Mitochondrial Import of Cytochrome c. in: Protein Synthesis and Targeting in Yeast

Dumont, M. E.;.

(A. Brown J. McCarthy, F. Sherman, and M. Tuite, ed.s) Springer-Verlag, N.Y. 1993; .

Co- and Post-Translational Processes and Mitochondrial Import of Yeast Cytochrome c.

Sherman, F.; Moerschell, R.P.; Tsunasawa, S.; Sternglanz, R.; Dumont, M.E. ;.

Translational Regulation of Gene Expression II (J. Ilan, ed.)Plenum Publishing, N.Y. 1993; .

Role of cytochrome c heme lyase in mitochondrial import and accumulation of cytochrome c in Saccharomyces cerevisiae.

Dumont ME, Cardillo TS, Hayes MK, Sherman F

Molecular and cellular biology.. 1991 November 11 (11):5487-96. Epub 1900 01 01.

Differential stability of two apo-isocytochromes c in the yeast Saccharomyces cerevisiae.

Dumont MD, Mathews AJ, Nall BT, Baim SB, Eustice DC, Sherman F

The Journal of biological chemistry.. 1990 February 15265 (5):2733-9. Epub 1900 01 01.

Protein targeting and protein secretion.

Dumont ME, Sherman F, Thomas DY

Genome. 1989 31 (2):1109-10. Epub 1900 01 01.

Coupling of heme attachment to import of cytochrome c into yeast mitochondria. Studies with heme lyase-deficient mitochondria and altered apocytochromes c.

Dumont ME, Ernst JF, Sherman F

The Journal of biological chemistry.. 1988 November 5263 (31):15928-37. Epub 1900 01 01.

The pH-dependent conformational change of diphtheria toxin.

Dumont ME, Richards FM

The Journal of biological chemistry.. 1988 February 5263 (4):2087-97. Epub 1900 01 01.

[Histopathology of the liver and kidney during malaria: relation to malaria-induced dyslipoproteinemia].

Dumont ME, Dei-Cas E, Maurois P, Slomianny C, Prensier G, Houcke-Lecomte M, Vernes A, Camus D

Annales de parasitologie humaine et comparée. 1988 63 (3):171-83. Epub 1900 01 01.

Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae.

Dumont ME, Ernst JF, Hampsey DM, Sherman F

The EMBO journal.. 1987 January 6 (1):235-41. Epub 1900 01 01.

Stability of transmembrane regions in bacteriorhodopsin studied by progressive proteolysis.

Dumont ME, Trewhella J, Engelman DM, Richards FM

The Journal of membrane biology.. 1985 88 (3):233-47. Epub 1900 01 01.

Insertion of apocytochrome c into lipid vesicles.

Dumont ME, Richards FM

The Journal of biological chemistry.. 1984 April 10259 (7):4147-56. Epub 1900 01 01.

Location of platinum binding sites on bacteriorhodopsin by electron diffraction.

Dumont ME, Wiggins JW, Hayward SB

Proceedings of the National Academy of Sciences of the United States of America.. 1981 May 78 (5):2947-51. Epub 1900 01 01.