Research Assistant Professor
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Department of Pediatrics, Hematology and Oncology (SMD)
Credentials
Post-doctoral Training & Residency
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY 1992 - 1994
Education
PhD | University of Rochester.Developmental Biology.1992
MS | University of Rochester.Developmental Biology.1987
BA | Reed College.Biology.1982
Research
Dr. Kingsley's research interests are: 1) Embryonic Hematopoiesis 2) Erythroid Differentiation 3) Ontogeny of Hematopoietic Progenitors 4) Erythroid Precursor Self Renewal
Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Forma...
Dr. Kingsley's research interests are: 1) Embryonic Hematopoiesis 2) Erythroid Differentiation 3) Ontogeny of Hematopoietic Progenitors 4) Erythroid Precursor Self Renewal
Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.
An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.
Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI
Nature.. 2000 February 17403 (6771):776-81. Epub 1900 01 01.
Miller RN, Dalamagas DG, Kingsley PD, Ettensohn CA
Mechanisms of development.. 1996 November 60 (1):3-12. Epub 1900 01 01.
Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes
Kingsley PD, Zinkin AS, Silver LS, Pendola FL, Palis J.
