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Paul J. Kammermeier, Ph.D.

Paul J. Kammermeier, Ph.D.

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About Me

Faculty Appointments

Associate Professor - Department of Pharmacology and Physiology (SMD)

Credentials

Post-doctoral Training & Residency

Research Fellow: National Institutes of Health (NIAAA), Rockville, MD. Advisor: Stephen R. Ikeda, M.D., Ph.D. 2002 - 2002

Post-Doctoral Associate: Guthrie Research Institute, Sayre, PA. Advisor: Stephen R. Ikeda, M.D., Ph.D. 1998 - 2002

Post-Doctoral Associate: University of Florida, Department of Physiology, Gainesville, FL. Principal Advisor: Philip Posner, Ph.D. 1997 - 1998

Education

PhD | Case Western Reserve Univ. Neuroscience. 1997

BS | Univ of Pittsburgh. Neuroscience. 1990

Research

My research interests center around G protein-coupled receptor function, particularly metabotropic glutamate receptors (mGluRs), and ion channel modulation in neurons. Naturally, this work has led me toward an interest in heterotrimeric G proteins, G protein-coupled receptors and factors that modula...
My research interests center around G protein-coupled receptor function, particularly metabotropic glutamate receptors (mGluRs), and ion channel modulation in neurons. Naturally, this work has led me toward an interest in heterotrimeric G proteins, G protein-coupled receptors and factors that modulate G protein signaling, such as the Regulators of G protein Signaling (RGS) proteins (see Kammermeier and Ikeda, 1999, Neuron 22:819). I have also become interested in the Homer family of scaffolding proteins, which I have shown to be important in the function and sub-cellular localization of metabotropic glutamate receptors (Kammermeier et al., 2000, Journal of Neuroscience 220:7238; Kammermeier, 2005, BMC Neuroscience 7:1). In the long term, my goal is to understand the mechanisms of regulation, localization and signaling that impart specificity to the various mGluRs, and how regulation of mGluR signaling alters synaptic strength in the nervous system.
In my laboratory, two basic experimental approaches are used. The first utilizes primary cultures of isolated SCG neurons for electrophysiological, molecular biological, immunofluorescence and RT-PCR experiments. The main technique used in this system is to examine the well-characterized ion channel modulatory pathways using patch-clamp electro-physiology as an assay for G protein coupled receptor activity. The neurons can be made to express various heterologous proteins (including mGluRs) via intranuclear injection of cDNA. I have recently used this system to examine mGluR-G protein coupling specificity, mGluR desensitization and the regulation of mGluR signaling and trafficking by various associated proteins.

The second approach is to use native neuronal cells. Currently, we are working with both primary isolated ON Bipolar cells from the rat retina and with rat hippocampal neurons to examine G protein coupling of mGluR6 and the effects of Homer protein regulation of mGluR5 at synapses, respectively. These systems allow us to ask questions about the physiological relevance in parallel with the more tractable, highly amenable SCG system described above.

Finally, we are beginning to incorporate other fluorescence-based techniques such as total internal reflection fluorescence (TIRF) microscopy (Kammermeier, 2005, BMC Neuroscience 7:1) to examine protein distribution in or very near the cell membrane.

Publications

Journal Articles

Unique pharmacology of mGlu homo- and heterodimers.

McCullock TW, Couch T, Kammermeier PJ

bioRxiv : the preprint server for biology.. 2024 November 19 Epub 11/19/2024.

Rat Sympathetic Neuron Calcium Channels Are Insensitive to Gabapentin.

Scott MB, Kammermeier PJ

Pharmaceuticals.. 2024 September 1917 (9)Epub 09/19/2024.

A PACAP-activated network for secretion requires coordination of Ca influx and Ca mobilization.

Chen X, Bell NA, Coffman BL, Rabino AA, Garcia-Mata R, Kammermeier PJ, Yule DI, Axelrod D, Smrcka AV, Giovannucci DR, Anantharam A

Molecular biology of the cell.. 2024 July 135 (7):ar92. Epub 05/17/2024.

Signaling Specificity and Kinetics of the Human Metabotropic Glutamate Receptors.

McCullock TW, Cardani LP, Kammermeier PJ

Molecular pharmacology.. 2024 January 10105 (2):104-115. Epub 01/10/2024.

A PACAP-activated network for secretion requires coordination of Ca influx and Ca mobilization.

Chen X, Bell NA, Coffman BL, Rabino AA, Garcia-Mata R, Kammermeier PJ, Yule DI, Axelrod D, Smrcka AV, Giovannucci DR, Anantharam A

bioRxiv : the preprint server for biology.. 2024 January 3 Epub 01/03/2024.

Signaling specificity and kinetics of the human metabotropic glutamate receptors.

McCullock TW, Cardani LP, Kammermeier PJ

bioRxiv : the preprint server for biology.. 2023 July 28 Epub 07/28/2023.

PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells.

Morales A, Mohan R, Chen X, Coffman BL, Bendahmane M, Watch L, West JL, Bakshi S, Traynor JR, Giovannucci DR, Kammermeier PJ, Axelrod D, Currie KPM, Smrcka AV, Anantharam A

The Journal of general physiology.. 2023 February 6155 (2)Epub 12/20/2022.

The evidence for and consequences of metabotropic glutamate receptor heterodimerization.

McCullock TW, Kammermeier PJ

Neuropharmacology.. 2021 November 1199 :108801. Epub 09/20/2021.

Stoichiometric analysis of protein complexes by cell fusion and single molecule imaging.

Singh A, Van Slyke AL, Sirenko M, Song A, Kammermeier PJ, Zipfel WR

Scientific reports.. 2020 September 1010 (1):14866. Epub 09/10/2020.

Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen.

McCullock TW, MacLean DM, Kammermeier PJ

PloS one.. 2020 15 (2):e0219886. Epub 02/05/2020.

Target validation: Weak selectivity of LY341495 for mGluR2 over mGluR4 makes glutamate a less selective agonist.

McCullock TW, Kammermeier PJ

Pharmacology research & perspectives.. 2019 June 7 (3):e00471. Epub 05/03/2019.

Ca2 channel subtype expression in rat sympathetic neurons is selectively regulated by ?? subunits.

Scott MB, Kammermeier PJ

Channels.. 2017 November 211 (6):555-573. Epub 09/21/2017.

Ion Channels: History, Diversity, and Impact.

Brenowitz S, Duguid I, Kammermeier PJ

Cold Spring Harbor protocols.. 2017 July 52017 (7):pdb.top092288. Epub 07/05/2017.

Dynamic Regulation of Homer Binding to Group I Metabotropic Glutamate Receptors by Preso1 and Converging Kinase Cascades.

Hu JH, Worley PF, Kammermeier PJ

The Journal of pharmacology and experimental therapeutics.. 2017 April 361 (1):122-129. Epub 02/08/2017.

Constitutive activity of metabotropic glutamate receptor 7.

Kammermeier PJ

BMC neuroscience.. 2015 March 2416 :17. Epub 03/24/2015.

Cooperative signaling between homodimers of metabotropic glutamate receptors 1 and 5.

Sevastyanova TN, Kammermeier PJ

Molecular pharmacology.. 2014 November 86 (5):492-504. Epub 08/11/2014.

The interaction between mGluR1 and the calcium channel Cav?.? preserves coupling in the presence of long Homer proteins.

Beqollari D, Kammermeier PJ

Neuropharmacology.. 2013 March 66 :302-10. Epub 05/30/2012.

Functional and pharmacological characteristics of metabotropic glutamate receptors 2/4 heterodimers.

Kammermeier PJ

Molecular pharmacology.. 2012 September 82 (3):438-47. Epub 05/31/2012.

Preso1 dynamically regulates group I metabotropic glutamate receptors.

Hu JH, Yang L, Kammermeier PJ, Moore CG, Brakeman PR, Tu J, Yu S, Petralia RS, Li Z, Zhang PW, Park JM, Dong X, Xiao B, Worley PF

Nature neuroscience.. 2012 June 15 (6):836-44. Epub 1900 01 01.

The orthosteric agonist 2-chloro-5-hydroxyphenylglycine activates mGluR5 and mGluR1 with similar efficacy and potency.

Kammermeier PJ

BMC pharmacology.. 2012 May 2912 :6. Epub 05/29/2012.

Venus fly trap domain of mGluR1 functions as a dominant negative against group I mGluR signaling.

Beqollari D, Kammermeier PJ

Journal of neurophysiology.. 2010 July 104 (1):439-48. Epub 05/12/2010.

C-terminal deletion of metabotropic glutamate receptor 1 selectively abolishes coupling to Galphaq.

Kammermeier PJ

European journal of pharmacology.. 2010 February 10627 (1-3):63-8. Epub 10/30/2009.

Altered G-protein coupling in an mGluR6 point mutant associated with congenital stationary night blindness.

Beqollari D, Betzenhauser MJ, Kammermeier PJ

Molecular pharmacology.. 2009 November 76 (5):992-7. Epub 08/07/2009.

Retina-specific GTPase accelerator RGS11/G beta 5S/R9AP is a constitutive heterotrimer selectively targeted to mGluR6 in ON-bipolar neurons.

Cao Y, Masuho I, Okawa H, Xie K, Asami J, Kammermeier PJ, Maddox DM, Furukawa T, Inoue T, Sampath AP, Martemyanov KA

The Journal of neuroscience : the official journal of the Society for Neuroscience.. 2009 July 2229 (29):9301-13. Epub 1900 01 01.

The mGlu(4) receptor allosteric modulator N-phenyl-7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxamide acts as a direct agonist at mGlu(6) receptors.

Beqollari D, Kammermeier PJ

European journal of pharmacology.. 2008 July 28589 (1-3):49-52. Epub 06/19/2008.

The mGlu(4) receptor allosteric modulator N-phenyl-7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxamide acts as a direct agonist at mGlu(6) receptors

Beqollari,D.; Kammermeier,P. J.;.

Eur.J.Pharmacol. 2008; 589(1-3): 49-52.

Endogenous homer proteins regulate metabotropic glutamate receptor signaling in neurons

Kammermeier,P. J.;.

J.Neurosci. 2008; 28(34): 8560-8567.

Homer 1a uncouples metabotropic glutamate receptor 5 from postsynaptic effectors.

Kammermeier PJ, Worley PF

Proceedings of the National Academy of Sciences of the United States of America.. 2007 April 3104 (14):6055-60. Epub 03/26/2007.

Homer 1a uncouples metabotropic glutamate receptor 5 from postsynaptic effectors

Kammermeier,P. J.; Worley,P. F.;.

Proc.Natl.Acad.Sci.U.S.A. 2007; 104(14): 6055-6060.

Surface clustering of metabotropic glutamate receptor 1 induced by long Homer proteins.

Kammermeier PJ

BMC neuroscience.. 2006 January 47 :1. Epub 01/04/2006.

G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells

Tian,L.; Kammermeier,P. J.;.

Vis.Neurosci. 2006; 23(6): 909-916.

G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells.

Tian L, Kammermeier PJ

Visual neuroscience.. 2006 23 (6):909-16. Epub 1900 01 01.

Activation of metabotropic glutamate receptor 1 dimers requires glutamate binding in both subunits.

Kammermeier PJ, Yun J

The Journal of pharmacology and experimental therapeutics.. 2005 February 312 (2):502-8. Epub 10/01/2004.

Specificity of metabotropic glutamate receptor 2 coupling to G proteins.

Kammermeier PJ, Davis MI, Ikeda SR

Molecular pharmacology.. 2003 January 63 (1):183-91. Epub 1900 01 01.

Metabotropic glutamate receptor expression in the rat superior cervical ganglion.

Kammermeier PJ, Ikeda SR

Neuroscience letters.. 2002 September 27330 (3):260-4. Epub 1900 01 01.

M current mystery messenger revealed?

Ikeda SR, Kammermeier PJ

Neuron.. 2002 August 135 (3):411-2. Epub 1900 01 01.

Desensitization of group I metabotropic glutamate receptors in rat sympathetic neurons.

Kammermeier PJ, Ikeda SR

Journal of neurophysiology.. 2002 April 87 (4):1669-76. Epub 1900 01 01.

A role for Seven in Absentia Homolog (Siah1a) in metabotropic glutamate receptor signaling.

Kammermeier PJ, Ikeda SR

BMC neuroscience.. 2001 2 :15. Epub 10/08/2001.

Homer proteins regulate coupling of group I metabotropic glutamate receptors to N-type calcium and M-type potassium channels.

Kammermeier PJ, Xiao B, Tu JC, Worley PF, Ikeda SR

The Journal of neuroscience : the official journal of the Society for Neuroscience.. 2000 October 120 (19):7238-45. Epub 1900 01 01.

A voltage-independent calcium current inhibitory pathway activated by muscarinic agonists in rat sympathetic neurons requires both Galpha q/11 and Gbeta gamma.

Kammermeier PJ, Ruiz-Velasco V, Ikeda SR

The Journal of neuroscience : the official journal of the Society for Neuroscience.. 2000 August 120 (15):5623-9. Epub 1900 01 01.

Heterologous expression of a green fluorescent protein-pertussis toxin S1 subunit fusion construct disrupts calcium channel modulation in rat superior cervical ganglion neurons.

Ikeda SR, Jeong SW, Kammermeier PJ, Ruiz-Velasco V, King MM

Neuroscience letters.. 1999 August 27271 (3):163-6. Epub 1900 01 01.

Expression of RGS2 alters the coupling of metabotropic glutamate receptor 1a to M-type K+ and N-type Ca2+ channels.

Kammermeier PJ, Ikeda SR

Neuron.. 1999 April 22 (4):819-29. Epub 1900 01 01.

Facilitation of L-type calcium current in thalamic neurons.

Kammermeier PJ, Jones SW

Journal of neurophysiology.. 1998 January 79 (1):410-7. Epub 1900 01 01.

High-voltage-activated calcium currents in neurons acutely isolated from the ventrobasal nucleus of the rat thalamus.

Kammermeier PJ, Jones SW

Journal of neurophysiology.. 1997 January 77 (1):465-75. Epub 1900 01 01.

Reevaluation of Ca2+ channel types and their modulation in bullfrog sympathetic neurons.

Elmslie KS, Kammermeier PJ, Jones SW

Neuron.. 1994 July 13 (1):217-28. Epub 1900 01 01.

Calcium current modulation in frog sympathetic neurones: L-current is relatively insensitive to neurotransmitters.

Elmslie KS, Kammermeier PJ, Jones SW

The Journal of physiology.. 1992 October 456 :107-23. Epub 1900 01 01.