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Alan E. Senior, Ph.D.

Alan E. Senior, Ph.D.

About Me

Faculty Appointments

Professor Emeritus - Department of Biochemistry and Biophysics (SMD)

Credentials

Education

DSc | U. Newcastle-On-Tyne. Distinguished Research. 1988

PhD | UK-Med Sch U Newcastle-On-Tyne. Pharmacology. 1967

BS | UK-Med Sch U Newcastle-On-Tyne. Physiology. 1964

Research

Multi drug resistance in cancer; ATP synthesis in bacteria and mitochondria.

(1) Multidrug resistance in cancer. Cancer chemotherapy often fails because the tumor develops resistance to multiple drugs simultaneously. The culprit is multidrug-resistance protein (mdr) also known as P-glycoprote...
Multi drug resistance in cancer; ATP synthesis in bacteria and mitochondria.

(1) Multidrug resistance in cancer. Cancer chemotherapy often fails because the tumor develops resistance to multiple drugs simultaneously. The culprit is multidrug-resistance protein (mdr) also known as P-glycoprotein. It is located in plasma membranes, and uses ATP to transport drugs out of cells. We have generated a cell line which greatly overexpresses mdr, from which enriched plasma membranes and purified protein are readily obtained. The long-term goal is to learn ways to disable or circumvent mdr protein in cancer cells.

(2) ATP synthesis and hydrolysis in F1Fo-ATP synthase. ATP synthase is the smallest known rotary motor. It uses subunit rotation to drive ATP synthesis, and ATP hydrolysis to drive subunit rotation. The subunit rotation is linked in turn to utilization or generation of a membrane proton gradient. We are using a variety of probes to study the mechanism of catalysis and to elucidate the molecular linkage between catalysis and subunit rotation. We use the E. coli enzyme and a range of molecular genetics, biochemical and biophysical techniques. In recent work we have focussed on application of fluoroscent probes of cataytic sites which have yielded rapid and sensitive information about mechanism.

Publications

Journal Articles

Two ATPases.

Senior AE

The Journal of biological chemistry.. 2012 August 31287 (36):30049-62. Epub 07/20/2012.

Reaction chemistry ABC-style.

Senior AE

Proceedings of the National Academy of Sciences of the United States of America.. 2011 September 13108 (37):15015-6. Epub 08/26/2011.

Role of {alpha}-subunit VISIT-DG sequence residues Ser-347 and Gly-351 in the catalytic sites of Escherichia coli ATP synthase.

Li W, Brudecki LE, Senior AE, Ahmad Z

The Journal of biological chemistry.. 2009 April 17284 (16):10747-54. Epub 02/23/2009.

Nucleotide-induced structural changes in P-glycoprotein observed by electron microscopy.

Lee JY, Urbatsch IL, Senior AE, Wilkens S

The Journal of biological chemistry.. 2008 February 29283 (9):5769-79. Epub 12/19/2007.

ATP synthase: motoring to the finish line.

Senior AE

Cell.. 2007 July 27130 (2):220-1. Epub 1900 01 01.

Mutational analysis of conserved aromatic residues in the A-loop of the ABC transporter ABCB1A (mouse Mdr3).

Carrier I, Urbatsch IL, Senior AE, Gros P

FEBS letters.. 2007 January 23581 (2):301-8. Epub 01/03/2007.

Inhibition of the ATPase activity of Escherichia coli ATP synthase by magnesium fluoride.

Ahmad Z, Senior AE

FEBS letters.. 2006 January 23580 (2):517-20. Epub 12/28/2005.

Expression, purification, and characterization of cysteine-free mouse P-glycoprotein.

Tombline G, Urbatsch IL, Virk N, Muharemagic A, White LB, Senior AE

Archives of biochemistry and biophysics.. 2006 January 1445 (1):124-8. Epub 11/28/2005.

Identification of phosphate binding residues of Escherichia coli ATP synthase.

Ahmad Z, Senior AE

Journal of bioenergetics and biomembranes.. 2005 December 37 (6):437-40. Epub 1900 01 01.

The occluded nucleotide conformation of p-glycoprotein.

Tombline G, Senior AE

Journal of bioenergetics and biomembranes.. 2005 December 37 (6):497-500. Epub 1900 01 01.

Nucleotide binding to the multidrug resistance P-glycoprotein as studied by ESR spectroscopy.

Delannoy S, Urbatsch IL, Tombline G, Senior AE, Vogel PD

Biochemistry.. 2005 October 2544 (42):14010-9. Epub 1900 01 01.

Involvement of the "occluded nucleotide conformation" of P-glycoprotein in the catalytic pathway.

Tombline G, Muharemagi? A, White LB, Senior AE

Biochemistry.. 2005 September 2744 (38):12879-86. Epub 1900 01 01.

Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy.

Wilkens S, Borchardt D, Weber J, Senior AE

Biochemistry.. 2005 September 644 (35):11786-94. Epub 1900 01 01.

Modulation of charge in the phosphate binding site of Escherichia coli ATP synthase.

Ahmad Z, Senior AE

The Journal of biological chemistry.. 2005 July 29280 (30):27981-9. Epub 06/06/2005.

Involvement of ATP synthase residues alphaArg-376, betaArg-182, and betaLys-155 in Pi binding.

Ahmad Z, Senior AE

FEBS letters.. 2005 January 17579 (2):523-8. Epub 1900 01 01.

Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues.

Tombline G, Bartholomew LA, Tyndall GA, Gimi K, Urbatsch IL, Senior AE

The Journal of biological chemistry.. 2004 November 5279 (45):46518-26. Epub 08/23/2004.

Role of betaAsn-243 in the phosphate-binding subdomain of catalytic sites of Escherichia coli F(1)-ATPase.

Ahmad Z, Senior AE

The Journal of biological chemistry.. 2004 October 29279 (44):46057-64. Epub 08/20/2004.

Mutagenesis of residue betaArg-246 in the phosphate-binding subdomain of catalytic sites of Escherichia coli F1-ATPase.

Ahmad Z, Senior AE

The Journal of biological chemistry.. 2004 July 23279 (30):31505-13. Epub 05/18/2004.

Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly.

Tombline G, Bartholomew LA, Urbatsch IL, Senior AE

The Journal of biological chemistry.. 2004 July 23279 (30):31212-20. Epub 05/24/2004.

Analysis of sequence determinants of F1Fo-ATP synthase in the N-terminal region of alpha subunit for binding of delta subunit.

Weber J, Muharemagic A, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 2004 June 11279 (24):25673-9. Epub 04/06/2004.

Quantitative determination of direct binding of b subunit to F1 in Escherichia coli F1F0-ATP synthase.

Weber J, Wilke-Mounts S, Nadanaciva S, Senior AE

The Journal of biological chemistry.. 2004 March 19279 (12):11253-8. Epub 01/13/2004.

Synergy between conserved ABC signature Ser residues in P-glycoprotein catalysis.

Tombline G, Bartholomew L, Gimi K, Tyndall GA, Senior AE

The Journal of biological chemistry.. 2004 February 13279 (7):5363-73. Epub 11/24/2003.

Happy motoring with ATP synthase.

Senior AE, Weber J

Nature structural & molecular biology.. 2004 February 11 (2):110-2. Epub 1900 01 01.

Fluorescent probes applied to catalytic cooperativity in ATP synthase.

Weber J, Senior AE

Methods in enzymology.. 2004 380 :132-52. Epub 1900 01 01.

P-glycoprotein catalytic mechanism: studies of the ADP-vanadate inhibited state.

Urbatsch IL, Tyndall GA, Tombline G, Senior AE

The Journal of biological chemistry.. 2003 June 20278 (25):23171-9. Epub 04/01/2003.

ATP synthesis driven by proton transport in F1F0-ATP synthase.

Weber J, Senior AE

FEBS letters.. 2003 June 12545 (1):61-70. Epub 1900 01 01.

F1F0-ATP synthase. Binding of delta subunit to a 22-residue peptide mimicking the N-terminal region of alpha subunit.

Weber J, Muharemagic A, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 2003 April 18278 (16):13623-6. Epub 02/20/2003.

Identification of the F1-binding surface on the delta-subunit of ATP synthase.

Weber J, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 2003 April 11278 (15):13409-16. Epub 01/29/2003.

Projection structure of P-glycoprotein by electron microscopy. Evidence for a closed conformation of the nucleotide binding domains.

Lee JY, Urbatsch IL, Senior AE, Wilkens S

The Journal of biological chemistry.. 2002 October 18277 (42):40125-31. Epub 08/05/2002.

Quantitative determination of binding affinity of delta-subunit in Escherichia coli F1-ATPase: effects of mutation, Mg2+, and pH on Kd.

Weber J, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 2002 May 24277 (21):18390-6. Epub 02/25/2002.

The molecular mechanism of ATP synthesis by F1F0-ATP synthase.

Senior AE, Nadanaciva S, Weber J

Biochimica et biophysica acta.. 2002 February 151553 (3):188-211. Epub 1900 01 01.

Cysteine-reactive fluorescence probes of catalytic sites of ATP synthase.

Weber J, Bijol V, Wilke-Mounts S, Senior AE

Archives of biochemistry and biophysics.. 2002 January 1397 (1):1-10. Epub 1900 01 01.

Bi-site catalysis in F1-ATPase: does it exist?

Weber J, Senior AE

The Journal of biological chemistry.. 2001 September 21276 (38):35422-8. Epub 07/12/2001.

Cysteines 431 and 1074 are responsible for inhibitory disulfide cross-linking between the two nucleotide-binding sites in human P-glycoprotein.

Urbatsch IL, Gimi K, Wilke-Mounts S, Lerner-Marmarosh N, Rousseau ME, Gros P, Senior AE

The Journal of biological chemistry.. 2001 July 20276 (29):26980-7. Epub 05/16/2001.

Purification and characterization of N-glycosylation mutant mouse and human P-glycoproteins expressed in Pichia pastoris cells.

Urbatsch IL, Wilke-Mounts S, Gimi K, Senior AE

Archives of biochemistry and biophysics.. 2001 April 1388 (1):171-7. Epub 1900 01 01.

ATP-driven rotation of the gamma subunit in F(1)-ATPase.

Weber J, Nadanaciva S, Senior AE

FEBS letters.. 2000 October 13483 (1):1-5. Epub 1900 01 01.

Investigation of the role of glutamine-471 and glutamine-1114 in the two catalytic sites of P-glycoprotein.

Urbatsch IL, Gimi K, Wilke-Mounts S, Senior AE

Biochemistry.. 2000 October 339 (39):11921-7. Epub 1900 01 01.

The catalytic transition state in ATP synthase.

Senior AE, Weber J, Nadanaciva S

Journal of bioenergetics and biomembranes.. 2000 October 32 (5):523-9. Epub 1900 01 01.

Conserved walker A Ser residues in the catalytic sites of P-glycoprotein are critical for catalysis and involved primarily at the transition state step.

Urbatsch IL, Gimi K, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 2000 August 11275 (32):25031-8. Epub 1900 01 01.

Functional analysis of a tryptophan-less P-glycoprotein: a tool for tryptophan insertion and fluorescence spectroscopy.

Kwan T, Loughrey H, Brault M, Gruenheid S, Urbatsch IL, Senior AE, Gros P

Molecular pharmacology.. 2000 July 58 (1):37-47. Epub 1900 01 01.

ATP synthase: what we know about ATP hydrolysis and what we do not know about ATP synthesis.

Weber J, Senior AE

Biochimica et biophysica acta.. 2000 May 311458 (2-3):300-9. Epub 1900 01 01.

Rate acceleration of ATP hydrolysis by F(1)F(o)-ATP synthase.

Senior AE, Nadanaciva S, Weber J

The Journal of experimental biology.. 2000 January 203 (Pt 1):35-40. Epub 1900 01 01.

The catalytic transition state in ATP synthase

Senior, A.E.; Nadanaciva, S.; Weber, J.;.

J. Bioenerg. Biomembr. 2000; 32(3): 523-529.

Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.

Lerner-Marmarosh N, Gimi K, Urbatsch IL, Gros P, Senior AE

The Journal of biological chemistry.. 1999 December 3274 (49):34711-8. Epub 1900 01 01.

Importance of F1-ATPase residue alpha-Arg-376 for catalytic transition state stabilization.

Nadanaciva S, Weber J, Wilke-Mounts S, Senior AE

Biochemistry.. 1999 November 2338 (47):15493-9. Epub 1900 01 01.

Effect of the epsilon-subunit on nucleotide binding to Escherichia coli F1-ATPase catalytic sites.

Weber J, Dunn SD, Senior AE

The Journal of biological chemistry.. 1999 July 2274 (27):19124-8. Epub 1900 01 01.

The role of beta-Arg-182, an essential catalytic site residue in Escherichia coli F1-ATPase.

Nadanaciva S, Weber J, Senior AE

Biochemistry.. 1999 June 1538 (24):7670-7. Epub 1900 01 01.

Binding of the transition state analog MgADP-fluoroaluminate to F1-ATPase.

Nadanaciva S, Weber J, Senior AE

The Journal of biological chemistry.. 1999 March 12274 (11):7052-8. Epub 1900 01 01.

Residues in P-glycoprotein catalytic sites that react with the inhibitor 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

Senior AE, Gros P, Urbatsch IL

Archives of biochemistry and biophysics.. 1998 September 1357 (1):121-5. Epub 1900 01 01.

Tryptophan substitutions surrounding the nucleotide in catalytic sites of F1-ATPase.

Weber J, Wilke-Mounts S, Hammond ST, Senior AE

Biochemistry.. 1998 September 137 (35):12042-50. Epub 1900 01 01.

Catalytic mechanism of P-glycoprotein.

Senior AE

Acta physiologica Scandinavica. Supplementum. 1998 August 643 :213-8. Epub 1900 01 01.

Catalytic site nucleotide binding and hydrolysis in F1F0-ATP synthase.

Löbau S, Weber J, Senior AE

Biochemistry.. 1998 July 2837 (30):10846-53. Epub 1900 01 01.

Effects of cardiovascular drugs on ATPase activity of P-glycoprotein in plasma membranes and in purified reconstituted form.

Rebbeor JF, Senior AE

Biochimica et biophysica acta.. 1998 February 21369 (1):85-93. Epub 1900 01 01.

P-glycoprotein shows strong catalytic cooperativity between the two nucleotide sites.

Senior AE, Bhagat S

Biochemistry.. 1998 January 2037 (3):831-6. Epub 1900 01 01.

Mg2+ coordination in catalytic sites of F1-ATPase.

Weber J, Hammond ST, Wilke-Mounts S, Senior AE

Biochemistry.. 1998 January 1337 (2):608-14. Epub 1900 01 01.

ATPase activity of Chinese hamster P-glycoprotein.

Senior AE, al-Shawi MK, Urbatsch IL

Methods in enzymology.. 1998 292 :514-23. Epub 1900 01 01.

Photoaffinity labelling of P-glycoprotein catalytic sites.

Sankaran B, Bhagat S, Senior AE

FEBS letters.. 1997 November 3417 (1):119-22. Epub 1900 01 01.

Binding of TNP-ATP and TNP-ADP to the non-catalytic sites of Escherichia coli F1-ATPase.

Weber J, Senior AE

FEBS letters.. 1997 July 21412 (1):169-72. Epub 1900 01 01.

Inhibition of P-glycoprotein ATPase activity by beryllium fluoride.

Sankaran B, Bhagat S, Senior AE

Biochemistry.. 1997 June 336 (22):6847-53. Epub 1900 01 01.

ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR.

Senior AE, Gadsby DC

Seminars in cancer biology.. 1997 June 8 (3):143-50. Epub 1900 01 01.

Inhibition of P-glycoprotein ATPase activity by procedures involving trapping of nucleotide in catalytic sites.

Sankaran B, Bhagat S, Senior AE

Archives of biochemistry and biophysics.. 1997 May 1341 (1):160-9. Epub 1900 01 01.

Catalytic mechanism of F1-ATPase.

Weber J, Senior AE

Biochimica et biophysica acta.. 1997 March 281319 (1):19-58. Epub 1900 01 01.

Nucleotide occupancy of F1-ATPase catalytic sites under crystallization conditions.

Löbau S, Weber J, Senior AE

FEBS letters.. 1997 March 3404 (1):15-8. Epub 1900 01 01.

F1-ATPase, roles of three catalytic site residues.

Löbau S, Weber J, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 1997 February 7272 (6):3648-56. Epub 1900 01 01.

F1F0-ATP synthase: development of direct optical probes of the catalytic mechanism.

Weber J, Senior AE

Biochimica et biophysica acta.. 1996 July 181275 (1-2):101-4. Epub 1900 01 01.

Binding and hydrolysis of TNP-ATP by Escherichia coli F1-ATPase.

Weber J, Senior AE

The Journal of biological chemistry.. 1996 February 16271 (7):3474-7. Epub 1900 01 01.

The catalytic cycle of P-glycoprotein.

Senior AE, al-Shawi MK, Urbatsch IL

FEBS letters.. 1995 December 27377 (3):285-9. Epub 1900 01 01.

Mutagenesis and reversion analysis of residue Met-209 of the beta-subunit of Escherichia coli ATP synthase.

Wilke-Mounts S, Pagan J, Senior AE

Archives of biochemistry and biophysics.. 1995 December 1324 (1):153-8. Epub 1900 01 01.

Both P-glycoprotein nucleotide-binding sites are catalytically active.

Urbatsch IL, Sankaran B, Bhagat S, Senior AE

The Journal of biological chemistry.. 1995 November 10270 (45):26956-61. Epub 1900 01 01.

Catalytic mechanism of Escherichia coli F1-ATPase.

Senior AE, Weber J, al-Shawi MK

Biochemical Society transactions.. 1995 November 23 (4):747-52. Epub 1900 01 01.

alpha-Aspartate 261 is a key residue in noncatalytic sites of Escherichia coli F1-ATPase.

Weber J, Bowman C, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 1995 September 8270 (36):21045-9. Epub 1900 01 01.

P-glycoprotein is stably inhibited by vanadate-induced trapping of nucleotide at a single catalytic site.

Urbatsch IL, Sankaran B, Weber J, Senior AE

The Journal of biological chemistry.. 1995 August 18270 (33):19383-90. Epub 1900 01 01.

ATP-dependent inactivation of the beta-Ser339Cys mutant F1-ATPase from Escherichia coli by N-ethylmaleimide.

Schmidt G, Senior AE

Biochemistry.. 1995 August 134 (30):9694-9. Epub 1900 01 01.

Location and properties of pyrophosphate-binding sites in Escherichia coli F1-ATPase.

Weber J, Senior AE

The Journal of biological chemistry.. 1995 May 26270 (21):12653-8. Epub 1900 01 01.

Inhibition of the ATPase activity of P-glycoprotein by porphyrin photosensitization of multidrug-resistant cells in vitro.

Gibson SL, al-Shawi MK, Senior AE, Hile R

Photochemistry and photobiology.. 1995 April 61 (4):390-6. Epub 1900 01 01.

ATP hydrolysis by multidrug-resistance protein from Chinese hamster ovary cells.

Senior AE, al-Shawi MK, Urbatsch IL

Journal of bioenergetics and biomembranes.. 1995 February 27 (1):31-6. Epub 1900 01 01.

Effects of lipids on ATPase activity of purified Chinese hamster P-glycoprotein.

Urbatsch IL, Senior AE

Archives of biochemistry and biophysics.. 1995 January 10316 (1):135-40. Epub 1900 01 01.

lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies.

Grell E, Lewitzki E, Bremer C, Kramer-Schmitt S, Weber J, Senior AE

Journal of fluorescence.. 1994 September 4 (3):247-50. Epub 1900 01 01.

Cooperativity and stoichiometry of substrate binding to the catalytic sites of Escherichia coli F1-ATPase. Effects of magnesium, inhibitors, and mutation.

Weber J, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 1994 August 12269 (32):20462-7. Epub 1900 01 01.

Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein.

Urbatsch IL, al-Shawi MK, Senior AE

Biochemistry.. 1994 June 1433 (23):7069-76. Epub 1900 01 01.

Tryptophan fluorescence provides a direct probe of nucleotide binding in the noncatalytic sites of Escherichia coli F1-ATPase.

Weber J, Wilke-Mounts S, Grell E, Senior AE

The Journal of biological chemistry.. 1994 April 15269 (15):11261-8. Epub 1900 01 01.

Covalent inhibitors of P-glycoprotein ATPase activity.

al-Shawi MK, Urbatsch IL, Senior AE

The Journal of biological chemistry.. 1994 March 25269 (12):8986-92. Epub 1900 01 01.

Tryptophan-free Escherichia coli F1-ATPase.

Wilke-Mounts S, Weber J, Grell E, Senior AE

Archives of biochemistry and biophysics.. 1994 March 309 (2):363-8. Epub 1900 01 01.

Mutagenesis of subunit delta from Escherichia coli F1F0-ATP synthase.

Hazard AL, Senior AE

The Journal of biological chemistry.. 1994 January 7269 (1):418-26. Epub 1900 01 01.

Defective energy coupling in delta-subunit mutants of Escherichia coli F1F0-ATP synthase.

Hazard AL, Senior AE

The Journal of biological chemistry.. 1994 January 7269 (1):427-32. Epub 1900 01 01.

Lin-Benzo-ATP and -ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

Grell, E.; Lewitzki, E.; Bremer C.; Kramer-Schmitt S.; Weber, J.; Senior A.E;.

J. Fluorescence. 1994; 4(3): 247-250.

Specific placement of tryptophan in the catalytic sites of Escherichia coli F1-ATPase provides a direct probe of nucleotide binding: maximal ATP hydrolysis occurs with three sites occupied.

Weber J, Wilke-Mounts S, Lee RS, Grell E, Senior AE

The Journal of biological chemistry.. 1993 September 25268 (27):20126-33. Epub 1900 01 01.

Lysine 155 in beta-subunit is a catalytic residue of Escherichia coli F1 ATPase.

Senior AE, Wilke-Mounts S, al-Shawi MK

The Journal of biological chemistry.. 1993 April 5268 (10):6989-94. Epub 1900 01 01.

Combined application of site-directed mutagenesis, 2-azido-ATP labeling, and lin-benzo-ATP binding to study the noncatalytic sites of Escherichia coli F1-ATPase.

Weber J, Lee RS, Wilke-Mounts S, Grell E, Senior AE

The Journal of biological chemistry.. 1993 March 25268 (9):6241-7. Epub 1900 01 01.

Characterization of the adenosine triphosphatase activity of Chinese hamster P-glycoprotein.

al-Shawi MK, Senior AE

The Journal of biological chemistry.. 1993 February 25268 (6):4197-206. Epub 1900 01 01.

Further examination of seventeen mutations in Escherichia coli F1-ATPase beta-subunit.

Senior AE, al-Shawi MK

The Journal of biological chemistry.. 1992 October 25267 (30):21471-8. Epub 1900 01 01.

Catalytic sites of Escherichia coli F1-ATPase.

Senior AE

Journal of bioenergetics and biomembranes.. 1992 October 24 (5):479-84. Epub 1900 01 01.

F1-ATPase with cysteine instead of serine at residue 373 of the alpha subunit.

Lee RS, Wilke-Mounts S, Senior AE

Archives of biochemistry and biophysics.. 1992 September 297 (2):334-9. Epub 1900 01 01.

Catalytic properties of Escherichia coli F1-ATPase depleted of endogenous nucleotides.

Senior AE, Lee RS, al-Shawi MK, Weber J

Archives of biochemistry and biophysics.. 1992 September 297 (2):340-4. Epub 1900 01 01.

Investigation of the aurovertin binding site of Escherichia coli F1-ATPase by fluorescence spectroscopy and site-directed mutagenesis.

Weber J, Lee RS, Grell E, Senior AE

Biochemistry.. 1992 June 931 (22):5112-6. Epub 1900 01 01.

Catalytic sites of Escherichia coli F1-ATPase. Characterization of unisite catalysis at varied pH.

al-Shawi MK, Senior AE

Biochemistry.. 1992 January 2831 (3):878-85. Epub 1900 01 01.

Effects of dimethyl sulfoxide on catalysis in Escherichia coli F1-ATPase.

al-Shawi MK, Senior AE

Biochemistry.. 1992 January 2831 (3):886-91. Epub 1900 01 01.

On the location and function of tyrosine beta 331 in the catalytic site of Escherichia coli F1-ATPase.

Weber J, Lee RS, Grell E, Wise JG, Senior AE

The Journal of biological chemistry.. 1992 January 25267 (3):1712-8. Epub 1900 01 01.

Characterization of Escherichia coli ATP synthase beta-subunit mutations using a chromosomal deletion strain.

Lee RS, Pagan J, Wilke-Mounts S, Senior AE

Biochemistry.. 1991 July 1630 (28):6842-7. Epub 1900 01 01.

Adenosine triphosphatase and nucleotide binding activity of isolated beta-subunit preparations from Escherichia coli F1F0-ATP synthase.

al-Shawi MK, Parsonage D, Senior AE

The Journal of biological chemistry.. 1990 April 5265 (10):5595-601. Epub 1900 01 01.

Thermodynamic analyses of the catalytic pathway of F1-ATPase from Escherichia coli. Implications regarding the nature of energy coupling by F1-ATPases.

al-Shawi MK, Parsonage D, Senior AE

The Journal of biological chemistry.. 1990 March 15265 (8):4402-10. Epub 1900 01 01.

Mutations in alpha-subunit of Escherichia coli F1-ATPase obtained by hydroxylamine-mutagenesis of plasmids carrying the uncA gene.

Pagan J, Senior AE

Archives of biochemistry and biophysics.. 1990 March 277 (2):283-9. Epub 1900 01 01.

The proton-translocating ATPase of Escherichia coli.

Senior AE

Annual review of biophysics and biophysical chemistry. 1990 19 :7-41. Epub 1900 01 01.

Kinetic characterization of the unisite catalytic pathway of seven beta-subunit mutant F1-ATPases from Escherichia coli.

al-Shawi MK, Parsonage D, Senior AE

The Journal of biological chemistry.. 1989 September 15264 (26):15376-83. Epub 1900 01 01.

Identification of a mutation in Escherichia coli F1-ATPase beta-subunit conferring resistance to aurovertin.

Lee RS, Pagan J, Satre M, Vignais PV, Senior AE

FEBS letters.. 1989 August 14253 (1-2):269-72. Epub 1900 01 01.

L-histidinol dehydrogenase, a Zn2+-metalloenzyme.

Grubmeyer C, Skiadopoulos M, Senior AE

Archives of biochemistry and biophysics.. 1989 August 1272 (2):311-7. Epub 1900 01 01.

Random mutagenesis of the gene for the beta-subunit of F1-ATPase from Escherichia coli.

Kironde FA, Parsonage D, Senior AE

The Biochemical journal.. 1989 April 15259 (2):421-6. Epub 1900 01 01.

Directed mutagenesis of the strongly conserved aspartate 242 in the beta-subunit of Escherichia coli proton-ATPase.

Al-Shawi MK, Parsonage D, Senior AE

The Journal of biological chemistry.. 1988 December 25263 (36):19633-9. Epub 1900 01 01.

Directed mutagenesis of the strongly conserved lysine 175 in the proposed nucleotide-binding domain of alpha-subunit from Escherichia coli F1-ATPase.

Rao R, Pagan J, Senior AE

The Journal of biological chemistry.. 1988 November 5263 (31):15957-63. Epub 1900 01 01.

E. coli F1-ATPase: site-directed mutagenesis of the beta-subunit.

Parsonage D, Wilke-Mounts S, Senior AE

FEBS letters.. 1988 May 9232 (1):111-4. Epub 1900 01 01.

Pyridoxal 5'-diphospho-5'-adenosine binds at a single site on isolated alpha-subunit from Escherichia coli F1-ATPase and specifically reacts with lysine 201.

Rao R, Cunningham D, Cross RL, Senior AE

The Journal of biological chemistry.. 1988 April 25263 (12):5640-5. Epub 1900 01 01.

A mutation in the alpha-subunit of F1-ATPase from Escherichia coli affects the binding of F1 to the membrane.

Maggio MB, Parsonage D, Senior AE

The Journal of biological chemistry.. 1988 April 5263 (10):4619-23. Epub 1900 01 01.

Directed mutations of the strongly conserved lysine 155 in the catalytic nucleotide-binding domain of beta-subunit of F1-ATPase from Escherichia coli.

Parsonage D, Al-Shawi MK, Senior AE

The Journal of biological chemistry.. 1988 April 5263 (10):4740-4. Epub 1900 01 01.

Directed mutagenesis of the dicyclohexylcarbodiimide-reactive carboxyl residues in beta-subunit of F1-ATPase of Escherichia coli.

Parsonage D, Wilke-Mounts S, Senior AE

Archives of biochemistry and biophysics.. 1988 February 15261 (1):222-5. Epub 1900 01 01.

ATP synthesis by oxidative phosphorylation.

Senior AE

Physiological reviews.. 1988 January 68 (1):177-231. Epub 1900 01 01.

The properties of hybrid F1-ATPase enzymes suggest that a cyclical catalytic mechanism involving three catalytic sites occurs.

Rao R, Senior AE

The Journal of biological chemistry.. 1987 December 25262 (36):17450-4. Epub 1900 01 01.

The defective proton-ATPase of uncA mutants of Escherichia coli. Identification by DNA sequencing of residues in the alpha-subunit which are essential for catalysis or normal assembly.

Maggio MB, Pagan J, Parsonage D, Hatch L, Senior AE

The Journal of biological chemistry.. 1987 July 5262 (19):8981-4. Epub 1900 01 01.

Directed mutagenesis of the beta-subunit of F1-ATPase from Escherichia coli.

Parsonage D, Wilke-Mounts S, Senior AE

The Journal of biological chemistry.. 1987 June 15262 (17):8022-6. Epub 1900 01 01.

The defective proton-ATPase of uncA mutants of Escherichia coli: ATP-binding and ATP-induced conformational change in mutant alpha-subunits.

Rao R, Perlin DS, Senior AE

Archives of biochemistry and biophysics.. 1987 June 255 (2):309-15. Epub 1900 01 01.

The defective proton-ATPase of uncD mutants of Escherichia coli. Identification by DNA sequencing of residues in the beta-subunit which are essential for catalysis or normal assembly.

Parsonage D, Duncan TM, Wilke-Mounts S, Kironde FA, Hatch L, Senior AE

The Journal of biological chemistry.. 1987 May 5262 (13):6301-7. Epub 1900 01 01.

Catalytic properties of the F1-adenosine triphosphatase from Escherichia coli K-12 and its genetic variants as revealed by 18O exchanges.

Wood JM, Wise JG, Senior AE, Futai M, Boyer PD

The Journal of biological chemistry.. 1987 February 15262 (5):2180-6. Epub 1900 01 01.

Amino acid substitutions in the epsilon-subunit of the F1F0-ATPase of Escherichia coli.

Cox GB, Hatch L, Webb D, Fimmel AL, Lin ZH, Senior AE, Gibson F

Biochimica et biophysica acta.. 1987 February 11890 (2):195-204. Epub 1900 01 01.

Structure of the nucleotide-binding domain in the beta-subunit of Escherichia coli F1-ATPase.

Duncan TM, Parsonage D, Senior AE

FEBS letters.. 1986 November 10208 (1):1-6. Epub 1900 01 01.

Inhibition of Escherichia coli H+-ATPase by venturicidin, oligomycin and ossamycin.

Perlin DS, Latchney LR, Senior AE

Biochimica et biophysica acta.. 1985 May 31807 (3):238-44. Epub 1900 01 01.

The defective proton-ATPase of uncD mutants of Escherichia coli. Two mutations which affect the catalytic mechanism.

Duncan TM, Senior AE

The Journal of biological chemistry.. 1985 April 25260 (8):4901-7. Epub 1900 01 01.

Functional effects and cross-reactivity of antibody to purified subunit b (uncF protein) of Escherichia coli proton-ATPase.

Perlin DS, Senior AE

Archives of biochemistry and biophysics.. 1985 February 1236 (2):603-11. Epub 1900 01 01.

The Proton-ATPase of Escherichia coli

Senior, A.E.;.

Current Topics in Membranes and Transport. 1985; 23(3): 135-151.

Specificity of the proton adenosinetriphosphatase of Escherichia coli for adenine, guanine, and inosine nucleotides in catalysis and binding.

Perlin DS, Latchney LR, Wise JG, Senior AE

Biochemistry.. 1984 October 923 (21):4998-5003. Epub 1900 01 01.

Disposition of polar and nonpolar residues on outer surfaces of transmembrane helical segments of proteins involved in proton translocation.

Senior AE

Archives of biochemistry and biophysics.. 1984 October 234 (1):138-43. Epub 1900 01 01.

Defective proton ATPase of uncA mutants of Escherichia coli. 5'-Adenylyl imidodiphosphate binding and ATP hydrolysis.

Wise JG, Latchney LR, Ferguson AM, Senior AE

Biochemistry.. 1984 March 2723 (7):1426-32. Epub 1900 01 01.

Purification of F1-ATPase with impaired catalytic activity from partial revertants of Escherichia coli uncA mutant strains.

Senior AE, Latchney LR, Ferguson AM, Wise JG

Archives of biochemistry and biophysics.. 1984 January 228 (1):49-53. Epub 1900 01 01.

Properties of F1-ATPase from the uncD412 mutant of Escherichia coli.

Wise JG, Duncan TM, Latchney LR, Cox DN, Senior AE

The Biochemical journal.. 1983 November 1215 (2):343-50. Epub 1900 01 01.

Oxidative phosphorylation by mutant Escherichia coli membranes with impaired proton permeability.

Cox GB, Jans DA, Gibson F, Langman L, Senior AE, Fimmel AL

The Biochemical journal.. 1983 October 15216 (1):143-50. Epub 1900 01 01.

Integration of F1 and the membrane sector of the proton-ATPase of Escherichia coli. Role of subunit "b" (uncF protein).

Perlin DS, Cox DN, Senior AE

The Journal of biological chemistry.. 1983 August 25258 (16):9793-800. Epub 1900 01 01.

The F1F0-ATPase of Escherichia coli. Substitution of proline by leucine at position 64 in the c-subunit causes loss of oxidative phosphorylation.

Fimmel AL, Jans DA, Langman L, James LB, Ash GR, Downie JA, Senior AE, Gibson F, Cox GB

The Biochemical journal.. 1983 August 1213 (2):451-8. Epub 1900 01 01.

Evidence that Mg2+- or Ca2+-activated adenosine triphosphatase in rat pancreas is a plasma-membrane ecto-enzyme.

Hamlyn JM, Senior AE

The Biochemical journal.. 1983 July 15214 (1):59-68. Epub 1900 01 01.

Secondary and tertiary structure of membrane proteins involved in proton translocation.

Senior AE

Biochimica et biophysica acta.. 1983 July 15726 (2):81-95. Epub 1900 01 01.

Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli.

Jans DA, Fimmel AL, Langman L, James LB, Downie JA, Senior AE, Ash GR, Gibson F, Cox GB

The Biochemical journal.. 1983 June 1211 (3):717-26. Epub 1900 01 01.

Oxidative phosphorylation in Escherichia coli. Characterization of mutant strains in which F1-ATPase contains abnormal beta-subunits.

Senior AE, Langman L, Cox GB, Gibson F

The Biochemical journal.. 1983 February 15210 (2):395-403. Epub 1900 01 01.

The proton-ATPase of bacteria and mitochondria.

Senior AE, Wise JG

The Journal of membrane biology.. 1983 73 (2):105-24. Epub 1900 01 01.

Trypsin cleavage of the alpha-subunit of beef heart F1-ATPase abolishes ATP synthesis and ATP-driven energy-transduction capabilities.

Skerrett KJ, Wise JG, Latchney LR, Senior AE

Biochimica et biophysica acta.. 1981 November 12638 (1):120-4. Epub 1900 01 01.

Assembly of the adenosine triphosphatase complex in Escherichia coli: assembly of F0 is dependent on the formation of specific F1 subunits.

Cox GB, Downie JA, Langman L, Senior AE, Ash G, Fayle DR, Gibson F

Journal of bacteriology.. 1981 October 148 (1):30-42. Epub 1900 01 01.

Membrane adenosine triphosphatase activities in rat pancreas.

Martin SS, Senior AE

Biochimica et biophysica acta.. 1980 November 4602 (2):401-18. Epub 1900 01 01.

Tight divalent cation-binding sites of soluble adenosine triphosphatase (F1) from beef heart mitochondria and Escherichia coli.

Senior AE, Richardson LV, Baker K, Wise JG

The Journal of biological chemistry.. 1980 August 10255 (15):7211-7. Epub 1900 01 01.

Tightly bound magnesium in mitochondrial adenosine triphosphatase from beef heart.

Senior AE

The Journal of biological chemistry.. 1979 November 25254 (22):11319-22. Epub 1900 01 01.

The uncA gene codes for the alpha-subunit of the adenosine triphosphatase of Escherichia coli. Electrophoretic analysis of uncA mutant strains.

Senior AE, Downie JA, Cox GB, Gibson F, Langman L, Fayle DR

The Biochemical journal.. 1979 April 15180 (1):103-9. Epub 1900 01 01.

Properties of membranes from mutant strains of Escherichia coli in which the beta-subunit of the adenosine triphosphatase is abnormal.

Senior AE, Fayle DR, Downie JA, Gibson F, Cox GB

The Biochemical journal.. 1979 April 15180 (1):111-8. Epub 1900 01 01.

Solubilization of adenosine triphosphatase from membranes of Escherichia coli: effect of p-aminobenzamidine.

Downie JA, Senior AE, Cox GB, Gibson F

Journal of bacteriology.. 1979 April 138 (1):87-91. Epub 1900 01 01.

A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli.

Downie JA, Senior AE, Gibson F, Cox GB

Journal of bacteriology.. 1979 February 137 (2):711-8. Epub 1900 01 01.

Prolactin binding to dissociated cells from rat mammary tumors and mammary gland.

Smith RD, Hilf R, Senior AE

Cancer biochemistry biophysics. 1979 3 (3):117-21. Epub 1900 01 01.

Oligomycin-sensitivity-conferring protein.

Senior AE

Methods in enzymology.. 1979 55 :391-7. Epub 1900 01 01.

The Mitochondrial ATPase". In "Membrane Proteins in Energy Transduction.

Senior, A.E.;.

Ed. R.A. Capaldi, Marcel Dekker, NY. 1979; : 233-278.

The effect of altered thyroid state on prolactin binding to liver and 7,12-dimethylbenz(a)anthracene-induced mammary tumors in rats.

Smith RD, Hilf R, Senior AE

Proceedings of the Society for Experimental Biology and Medicine.. 1978 September 158 (4):517-20. Epub 1900 01 01.

Tightly-bound ATP and ADP in reconstituted submitochondrial particles.

Leimgruber RM, Senior AE

Biochemical and biophysical research communications.. 1978 August 1483 (3):837-42. Epub 1900 01 01.

The Reversible Mitochondrial ATPase as a Model for the Resolution of a Membrane Complex". In "The Molecular Biology of Membranes

Senior, A.E.;.

Ed. Fleischer, S. et al., Plenum Press, NY. 1978; : 121-135.

Prolactin binding to 7,12-dimethylbenz(a)anthracene-induced mammary tumors and liver in diabetic rats.

Smith RD, Hilf R, Senior AE

Cancer research.. 1977 November 37 (11):4070-4. Epub 1900 01 01.

Prolactin binding to R3230AC mammary carcinoma and liver in hormone-treated and diabetic rats.

Smith RD, Hilf R, Senior AE

Cancer research.. 1977 February 37 (2):595-8. Epub 1900 01 01.

Removal of "tightly bound" nucleotides from soluble mitochondrial adenosine triphosphatase (F1).

Leimgruber RM, Senior AE

The Journal of biological chemistry.. 1976 November 25251 (22):7103-9. Epub 1900 01 01.

Removal of "tightly bound" nucleotides from phosphorylating submitochondrial particles.

Leimgruber RM, Senior AE

The Journal of biological chemistry.. 1976 November 25251 (22):7110-3. Epub 1900 01 01.

Prolactin binding to mammary gland, 7,12-dimethylbenz(a)-anthracene-induced mammary tumors, and liver in rats.

Smith RD, Hilf R, Senior AE

Cancer research.. 1976 October 36 (10):3726-31. Epub 1900 01 01.

Reaction of amino-phospholipids of the inner mitochondrial membrane with fluorodinitrobenzene and trinitrobenzenesulfonate.

Marinetti GV, Senior AE, Love R, Broadhurst CI

Chemistry and physics of lipids.. 1976 October 17 (2-3 SPEC NO):353-62. Epub 1900 01 01.

On the Mechanism of Inhibition of Fatty Acid Oxidation by Hypoglycin and by Pent-4-enoic acid". In "Hypoglycin.

Sherratt, H.S.A.; Holland, P.C.; Osmundsen , H.; Senior, A.E.;.

Ed. E.A. Kean, Academic Press, N.Y. 1976; : 127-14.

Activation and Photoinactivation of F1 by 4-fluoro, 3-nitrophenylazide". In "Electron Transfer Chains and Oxidative Phosphorylation.

Senior, A.E.; Tometsko, A.M.;.

North Holland/American Elsevier, N.Y. Ed. E. Quagliariello et al. 1975; : 155-160.

The structure of mitochondrial ATPase.

Senior AE

Biochimica et biophysica acta.. 1973 December 31301 (3):249-77. Epub 1900 01 01.

Methods for purification of each subunit of the mitochondrial oligomycin-insensitive adenosine triphosphatase.

Brooks JC, Senior AE

Biochemistry.. 1972 December 511 (25):4675-8. Epub 1900 01 01.

Conformational changes in membranous preparations of cytochrome oxidase.

Wakabayashi T, Senior AE, Hatase O, Hayashi H, Green DE

Journal of bioenergetics. 1972 August 3 (5):339-44. Epub 1900 01 01.

Biological membrane structure. 3. The lattice structure of membranous cytochrome oxidase.

Vanderkooi G, Senior AE, Capaldi RA, Hayashi H

Biochimica et biophysica acta.. 1972 July 3274 (1):38-48. Epub 1900 01 01.

Mechanism of inhibition of fatty acid oxidation by pent-4-enoic acid: 3-oxoacyl-coenzyme A thiolase as the possible site of inhibition.

Holland PC, Senior AE, Sherratt HS

The Biochemical journal.. 1972 April 127 (3):79P-80P. Epub 1900 01 01.

Studies on the mitochondrial oligomycin-insensitive ATPase. II. The relationship of the specific protein inhibitor to the ATPase.

Brooks JC, Senior AE

Archives of biochemistry and biophysics.. 1971 December 147 (2):467-70. Epub 1900 01 01.

The subunit composition of the mitochondrial oligomycin-insensitive ATPase.

Senior AE, Brooks JC

FEBS letters.. 1971 October 117 (2):327-329. Epub 1900 01 01.

Reassessment of the molecular weight of mitochondrial ATPase from beef heart.

Lambeth DO, Lardy HA, Senior AE, Brooks JC

FEBS letters.. 1971 October 117 (2):330-332. Epub 1900 01 01.

On the relationship between the oligomycin-sensitivity conferring protein and other mitochondrial coupling factors.

Senior AE

Journal of bioenergetics. 1971 August 2 (3):141-50. Epub 1900 01 01.

The isolation and properties of a peptide ionophore from beef heart mitochondria.

Blondin GA, DeCastro AF, Senior AE

Biochemical and biophysical research communications.. 1971 April 243 (1):28-35. Epub 1900 01 01.

Mode of Action of Hypoglycin and Related Compounds". In "Mechanisms of Toxicity,

Sherratt, H.S.A.; Holland, P.C; Marley, Judith; Senior, A.E.;.

Ed. W.N. Aldridge,MacMillan & Co., London, (REVIEW). 1971; : 205-218.

Mitochondrial "structural protein." A reassessment.

Senior AE, MacLennan DH

The Journal of biological chemistry.. 1970 October 10245 (19):5086-95. Epub 1900 01 01.

A comparison of the effects on blood glucose and ketone-body levels, and of the toxicities, of pent-4-enoic acid and four simple fatty acids.

Senior AE, Sherratt HS

The Journal of pharmacy and pharmacology.. 1969 February 21 (2):85-92. Epub 1900 01 01.

Biochemical effects of the hypoglycaemic compound pent--4-enoic acid and related non-hypoglycaemic fatty acids.

Senior AE, Robson B, Sherratt HS

The Biochemical journal.. 1968 December 110 (3):511-9. Epub 1900 01 01.

Biochemical effects of the hypoglycaemic compound pent-4-enoic acid and related non-hypoglycaemic fatty acids. Carbohydrate metabolism.

Senior AE, Sherratt HS

The Biochemical journal.. 1968 December 110 (3):521-7. Epub 1900 01 01.

The effect of pent-4-enoic acid and some simple related compounds on the oxidation of fatty acids by rat-liver mitochondria.

Senior AE, Sherratt HS

The Biochemical journal.. 1967 September 104 (3):56P. Epub 1900 01 01.