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Paul Kingsley, Ph.D.

Paul Kingsley, Ph.D.

Contact

Office (585) 275-5073

About Me

Faculty Appointments

Research Assistant Professor - Department of Pediatrics, Hematology and Oncology (SMD)

Credentials

Post-doctoral Training & Residency

Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY 1992 - 1994

Education

PhD | University of Rochester. Developmental Biology. 1992

MS | University of Rochester. Developmental Biology. 1987

BA | Reed College. Biology. 1982

Research

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Forma...
Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Publications

Journal Articles

An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease.

Zhao N, Michelucci A, Pietrangelo L, Malik S, Groom L, Leigh J, O'Connor TN, Takano T, Kingsley PD, Palis J, Boncompagni S, Protasi F, Dirksen RT

The EMBO journal.. 2024 October 17 Epub 10/17/2024.

Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia models.

Livada AC, McGrath KE, Malloy MW, Li C, Ture SK, Kingsley PD, Koniski AD, Vit LA, Nolan KE, Mickelsen D, Monette GE, Maurya P, Palis J, Morrell CN

The Journal of clinical investigation.. 2024 September 19 Epub 09/19/2024.

BMI1 regulates human erythroid self-renewal through both gene repression and gene activation.

McGrath KE, Koniski AD, Murphy K, Getman M, An HH, Schulz VP, Kim AR, Zhang B, Schofield TL, Papoin J, Blanc L, Kingsley PD, Westhoff CM, Gallagher PG, Chou ST, Steiner LA, Palis J

bioRxiv : the preprint server for biology.. 2024 February 6 Epub 02/06/2024.

Circulating primitive murine erythroblasts undergo complex proteomic and metabolomic changes during terminal maturation.

Nemkov T, Kingsley PD, Dzieciatkowska M, Malik J, McGrath K, Hansen KC, D'Alessandro A, Palis J

Blood advances.. 2022 May 246 (10):3072-3089. Epub 1900 01 01.

Long-acting PGE2 and Lisinopril Mitigate H-ARS.

Saunders J, Niswander LM, McGrath KE, Koniski A, Catherman SC, Ture SK, Medhora M, Kingsley PD, Calvi LM, Williams JP, Morrell CN, Palis J

Radiation research.. 2021 September 1196 (3):284-296. Epub 1900 01 01.

Mds1, an inducible Cre allele specific to adult-repopulating hematopoietic stem cells.

Zhang Y, McGrath KE, Ayoub E, Kingsley PD, Yu H, Fegan K, McGlynn KA, Rudzinskas S, Palis J, Perkins AS

Cell reports.. 2021 August 1736 (7):109562. Epub 1900 01 01.

Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis.

Jeffery NN, Davidson C, Peslak SA, Kingsley PD, Nakamura Y, Palis J, Bulger M

Epigenetics & chromatin.. 2021 July 3014 (1):37. Epub 07/30/2021.

A human cell atlas of fetal gene expression.

Cao J, O'Day DR, Pliner HA, Kingsley PD, Deng M, Daza RM, Zager MA, Aldinger KA, Blecher-Gonen R, Zhang F, Spielmann M, Palis J, Doherty D, Steemers FJ, Glass IA, Trapnell C, Shendure J

Science.. 2020 November 13370 (6518)Epub 1900 01 01.

Hyperacetylated chromatin domains mark cell type-specific genes and suggest distinct modes of enhancer function.

Fox S, Myers JA, Davidson C, Getman M, Kingsley PD, Frankiewicz N, Bulger M

Nature communications.. 2020 September 1111 (1):4544. Epub 09/11/2020.

Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development.

Dege C, Fegan KH, Creamer JP, Berrien-Elliott MM, Luff SA, Kim D, Wagner JA, Kingsley PD, McGrath KE, Fehniger TA, Palis J, Sturgeon CM

Developmental cell.. 2020 April 2053 (2):229-239.e7. Epub 03/19/2020.

Acute and late effects of combined internal and external radiation exposures on the hematopoietic system.

Calvi LM, Frisch BJ, Kingsley PD, Koniski AD, Love TM, Williams JP, Palis J

International journal of radiation biology.. 2019 November 95 (11):1447-1461. Epub 07/30/2019.

regulates age-dependent differences in murine platelet function.

Stolla MC, Catherman SC, Kingsley PD, Rowe RG, Koniski AD, Fegan K, Vit L, McGrath KE, Daley GQ, Palis J

Blood advances.. 2019 January 83 (1):72-82. Epub 1900 01 01.

FAM210B is an erythropoietin target and regulates erythroid heme synthesis by controlling mitochondrial iron import and ferrochelatase activity.

Yien YY, Shi J, Chen C, Cheung JT, Grillo AS, Shrestha R, Li L, Zhang X, Kafina MD, Kingsley PD, King MJ, Ablain J, Li H, Zon L, Palis J, Burke MD, Bauer DE, Orkin SH, Koehler CM, Phillips JD, Kaplan J, Ward DM, Lodish HF, Paw BH

The Journal of biological chemistry.. 2018 December 21293 (51):19797-19811. Epub 10/26/2018.

Microfluidic assay of the deformability of primitive erythroblasts.

Zhou S, Huang YS, Kingsley PD, Cyr KH, Palis J, Wan J

Biomicrofluidics.. 2017 September 11 (5):054112. Epub 10/23/2017.

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.

Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J

Scientific reports.. 2017 July 127 (1):5164. Epub 07/12/2017.

Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis.

Liddicoat BJ, Hartner JC, Piskol R, Ramaswami G, Chalk AM, Kingsley PD, Sankaran VG, Wall M, Purton LE, Seeburg PH, Palis J, Orkin SH, Lu J, Li JB, Walkley CR

Experimental hematology.. 2016 October 44 (10):947-63. Epub 07/01/2016.

Distinct Sources of Hematopoietic Progenitors Emerge before HSCs and Provide Functional Blood Cells in the Mammalian Embryo.

McGrath KE, Frame JM, Fegan KH, Bowen JR, Conway SJ, Catherman SC, Kingsley PD, Koniski AD, Palis J

Cell reports.. 2015 June 3011 (12):1892-904. Epub 06/18/2015.

Bmi-1 Regulates Extensive Erythroid Self-Renewal.

Kim AR, Olsen JL, England SJ, Huang YS, Fegan KH, Delgadillo LF, McGrath KE, Kingsley PD, Waugh RE, Palis J

Stem cell reports.. 2015 June 94 (6):995-1003. Epub 05/28/2015.

The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability.

Chung J, Bauer DE, Ghamari A, Nizzi CP, Deck KM, Kingsley PD, Yien YY, Huston NC, Chen C, Schultz IJ, Dalton AJ, Wittig JG, Palis J, Orkin SH, Lodish HF, Eisenstein RS, Cantor AB, Paw BH

Science signaling.. 2015 April 148 (372):ra34. Epub 04/14/2015.

SDF-1 dynamically mediates megakaryocyte niche occupancy and thrombopoiesis at steady state and following radiation injury.

Niswander LM, Fegan KH, Kingsley PD, McGrath KE, Palis J

Blood.. 2014 July 10124 (2):277-86. Epub 04/15/2014.

Ontogeny of erythroid gene expression.

Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J

Blood.. 2013 February 7121 (6):e5-e13. Epub 12/12/2012.

EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress.

Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J

Blood.. 2012 September 20120 (12):2501-11. Epub 08/13/2012.

An embryonic stage-specific enhancer within the murine ?-globin locus mediates domain-wide histone hyperacetylation.

Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M

Blood.. 2011 May 12117 (19):5207-14. Epub 02/14/2011.

A transient definitive erythroid lineage with unique regulation of the ?-globin locus in the mammalian embryo.

McGrath KE, Frame JM, Fromm GJ, Koniski AD, Kingsley PD, Little J, Bulger M, Palis J

Blood.. 2011 April 28117 (17):4600-8. Epub 03/04/2011.

Sublethal radiation injury uncovers a functional transition during erythroid maturation.

Peslak SA, Wenger J, Bemis JC, Kingsley PD, Frame JM, Koniski AD, Chen Y, Williams JP, McGrath KE, Dertinger SD, Palis J

Experimental hematology.. 2011 April 39 (4):434-45. Epub 02/01/2011.

Primitive erythropoiesis in the mammalian embryo.

Palis J, Malik J, McGrath KE, Kingsley PD

The International journal of developmental biology.. 2010 54 (6-7):1011-8. Epub 1900 01 01.

Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis.

Nilsson R, Schultz IJ, Pierce EL, Soltis KA, Naranuntarat A, Ward DM, Baughman JM, Paradkar PN, Kingsley PD, Culotta VC, Kaplan J, Palis J, Paw BH, Mootha VK

Cell metabolism.. 2009 August 10 (2):119-30. Epub 1900 01 01.

Reduced COX-2 expression in aged mice is associated with impaired fracture healing.

Naik, A.A.; Xie, C.; Zuscik, M.J.; Kingsley, P., Schwarz, E.M.; Awad, H.; Guldberg, R.; Drissi, H.; Puzas, J.E.; Boyce, B.; Zhang, X; O'Keefe, R.J.

J Bone Miner Res. 2009; 24: 251-264.

Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands.

Bullard T, Koek L, Roztocil E, Kingsley PD, Mirels L, Ovitt CE

Developmental biology.. 2008 August 1320 (1):72-8. Epub 04/23/2008.

Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus.

McGrath KE, Kingsley PD, Koniski AD, Porter RL, Bushnell TP, Palis J

Blood.. 2008 February 15111 (4):2409-17. Epub 11/21/2007.

A heme export protein is required for red blood cell differentiation and iron homeostasis.

Keel SB, Doty RT, Yang Z, Quigley JG, Chen J, Knoblaugh S, Kingsley PD, De Domenico I, Vaughn MB, Kaplan J, Palis J, Abkowitz JL

Science.. 2008 February 8319 (5864):825-8. Epub 1900 01 01.

"Maturational" globin switching in primary primitive erythroid cells.

Kingsley PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, Palis J

Blood.. 2006 February 15107 (4):1665-72. Epub 11/01/2005.

Sox6 directly silences epsilon globin expression in definitive erythropoiesis.

Yi Z, Cohen-Barak O, Hagiwara N, Kingsley PD, Fuchs DA, Erickson DT, Epner EM, Palis J, Brilliant MH

PLoS genetics.. 2006 February 2 (2):e14. Epub 02/03/2006.

Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis.

Wingert RA, Galloway JL, Barut B, Foott H, Fraenkel P, Axe JL, Weber GJ, Dooley K, Davidson AJ, Schmid B, Schmidt B, Paw BH, Shaw GC, Kingsley P, Palis J, Schubert H, Chen O, Kaplan J, Zon LI,

Nature.. 2005 August 18436 (7053):1035-39. Epub 1900 01 01.

Overlapping expression of Runx1(Cbfa2) and Runx2(Cbfa1) transcription factors supports cooperative induction of skeletal development.

Smith N, Dong Y, Lian JB, Pratap J, Kingsley PD, van Wijnen AJ, Stein JL, Schwarz EM, O'Keefe RJ, Stein GS, Drissi MH

Journal of cellular physiology.. 2005 April 203 (1):133-43. Epub 1900 01 01.

Yolk sac-derived primitive erythroblasts enucleate during mammalian embryogenesis.

Kingsley PD, Malik J, Fantauzzo KA, Palis J

Blood.. 2004 July 1104 (1):19-25. Epub 03/18/2004.

The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis

Ransom,D. G.; Bahary,N.; Niss,K.; Traver,D.; Burns,C.; Trede,N. S.; Paffett-Lugassy,N.; Saganic,W. J.; Lim,C. A.; Hersey,C.; Zhou,Y.; Barut,B. A.; Lin,S.; Kingsley,P. D.; Palis,J.; Orkin,S. H.; Zon,L. I.;.

PLoS Biol. 2004; 2(8): E237.

cdx4 mutants fail to specify blood progenitors and can be rescued by multiple hox genes.

Davidson AJ, Ernst P, Wang Y, Dekens MP, Kingsley PD, Palis J, Korsmeyer SJ, Daley GQ, Zon LI

Nature.. 2003 September 18425 (6955):300-6. Epub 1900 01 01.

Lineage analysis of the hemangioblast as defined by FLK1 and SCL expression.

Chung YS, Zhang WJ, Arentson E, Kingsley PD, Palis J, Choi K

Development.. 2002 December 129 (23):5511-20. Epub 1900 01 01.

Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitro.

Lacaud G, Gore L, Kennedy M, Kouskoff V, Kingsley P, Hogan C, Carlsson L, Speck N, Palis J, Keller G

Blood.. 2002 July 15100 (2):458-66. Epub 1900 01 01.

Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle.

Elble RC, Ji G, Nehrke K, DeBiasio J, Kingsley PD, Kotlikoff MI, Pauli BU

The Journal of biological chemistry.. 2002 May 24277 (21):18586-91. Epub 03/14/2002.

Osr2, a new mouse gene related to Drosophila odd-skipped, exhibits dynamic expression patterns during craniofacial, limb, and kidney development.

Lan Y, Kingsley PD, Cho ES, Jiang R

Mechanisms of development.. 2001 September 107 (1-2):175-9. Epub 1900 01 01.

Cloning and characterization of a ninth member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, ppGaNTase-T9.

Ten Hagen KG, Bedi GS, Tetaert D, Kingsley PD, Hagen FK, Balys MM, Beres TM, Degand P, Tabak LA

The Journal of biological chemistry.. 2001 May 18276 (20):17395-404. Epub 02/06/2001.

Subtractive hybridization reveals tissue-specific expression of ahnak during embryonic development.

Kingsley PD, McGrath KE, Maltby KM, Koniski AD, Ramchandran R, Palis J

Development, growth & differentiation.. 2001 April 43 (2):133-43. Epub 1900 01 01.

Diverse spatial expression patterns of UDP-GalNAc:polypeptide N-acetylgalactosaminyl-transferase family member mRNAs during mouse development.

Kingsley PD, Hagen KG, Maltby KM, Zara J, Tabak LA

Glycobiology.. 2000 December 10 (12):1317-23. Epub 1900 01 01.

Targeted deletion of Minpp1 provides new insight into the activity of multiple inositol polyphosphate phosphatase in vivo.

Chi H, Yang X, Kingsley PD, O'Keefe RJ, Puzas JE, Rosier RN, Shears SB, Reynolds PR

Molecular and cellular biology.. 2000 September 20 (17):6496-507. Epub 1900 01 01.

Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter.

Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI

Nature.. 2000 February 17403 (6771):776-81. Epub 1900 01 01.

A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein AML1-ETO-expressing mice, functions in hematopoietic cell differentiation.

Liu LQ, Ilaria R, Kingsley PD, Iwama A, van Etten RA, Palis J, Zhang DE

Molecular and cellular biology.. 1999 April 19 (4):3029-38. Epub 1900 01 01.

Characterization of human endotoxin lipopolysaccharide receptor CD14 expression in transgenic mice.

Hetherington CJ, Kingsley PD, Crocicchio F, Zhang P, Rabin MS, Palis J, Zhang DE

The Journal of immunology : official journal of the American Association of Immunologists.. 1999 January 1162 (1):503-9. Epub 1900 01 01.

Characterization of human endotoxin lipopolysaccharide receptor CD14 expression in transgenic mice.

Hetherington, C.J.; Kingsley, P.D.; Crocicchio, F.; Zhang, P.; Rabin, M.S.; Palis, J.; Zhang, D.E.

J Immunol. 1999; 163(1): 503.

Developmental expression of extracellular glutathione peroxidase suggests antioxidant roles in deciduum, visceral yolk sac, and skin.

Kingsley PD, Whitin JC, Cohen HJ, Palis J

Molecular reproduction and development.. 1998 April 49 (4):343-55. Epub 1900 01 01.

Inherent properties of somatic hypermutation as revealed by human non-productive VH6 immunoglobulin rearrangements.

Varade WS, Carnahan JA, Kingsley PD, Insel RA

Immunology.. 1998 February 93 (2):171-6. Epub 1900 01 01.

Expression of S9 and actin CyIIa mRNAs reveals dorso-ventral polarity and mesodermal sublineages in the vegetal plate of the sea urchin embryo.

Miller RN, Dalamagas DG, Kingsley PD, Ettensohn CA

Mechanisms of development.. 1996 November 60 (1):3-12. Epub 1900 01 01.

Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes

Kingsley PD, Zinkin AS, Silver LS, Pendola FL, Palis J.

Dev. Growth Diff. 1996; 38: 697-706.

Differential gene expression during early murine yolk sac development.

Palis J, Kingsley PD

Molecular reproduction and development.. 1995 September 42 (1):19-27. Epub 1900 01 01.

Initiation of hematopoiesis and vasculogenesis in murine yolk sac explants.

Palis J, McGrath KE, Kingsley PD

Blood.. 1995 July 186 (1):156-63. Epub 1900 01 01.

GRP2 proteins contain both CCHC zinc fingers and a cold shock domain.

Kingsley PD, Palis J

The Plant cell.. 1994 November 6 (11):1522-3. Epub 1900 01 01.

Immunochemical Analysis of Arylsulfatase Accumulation in Sea Urchin Embryos: (extracellular matrix/arylsulfatase/sea urchin embryo/tissue-specific gene products/sea urchin embryo/in situ hybridization).

Yang Q, Kingsley PD, Kozlowski DJ, Angerer RC, Angerer LM

Development, growth & differentiation.. 1993 April 35 (2):139-151. Epub 1900 01 01.

Major temporal and spatial patterns of gene expression during differentiation of the sea urchin embryo.

Kingsley PD, Angerer LM, Angerer RC

Developmental biology.. 1993 January 155 (1):216-34. Epub 1900 01 01.

Immunochemical analysis of arylsulfatase accumulation in sea urchin embryos

Yang Q, Kingsley PD, Kozlowski RC, Angerer RC, Angerer LM.

Dev Growth Differ. 1993; 35: 139-151.

Tissue-restricted accumulation of a ribosomal protein mRNA is not coordinated with rRNA transcription and precedes growth of the sea urchin pluteus larva.

Angerer LM, Yang Q, Liesveld J, Kingsley PD, Angerer RC

Developmental biology.. 1992 January 149 (1):27-40. Epub 1900 01 01.

Contributions of the spatial analysis of gene expression to the study of sea urchin development

Angerer RC, Reynolds SD, Grimwade J, Hurley DL, Yang Q, Kingsley PD, Gagnon ML, Palis J, Angerer LM.

Soc Exp Biol Seminars (Cambridge Press). 1990; 43: 69-95.

Human trabecular meshwork organ culture: morphology and glycosaminoglycan synthesis

Acott TS, Kingsley PD, Samples JR, Van Buskirk EM.

Inv Opthal Vis Sci. 1988; 29((1)): 90-100.

Fluctuation of intracranial pressure associated with the cardiac cycle.

Daley ML, Gallo AE, Gehling GF, Isom JB, Mauch W, Kingsley PD

Neurosurgery.. 1982 November 11 (5):617-21. Epub 1900 01 01.