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Chad Alan Galloway, Ph.D.

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Chad Alan Galloway, Ph.D.

About Me
Credentials
Research
Publications

About Me

Faculty Appointments

Research Assistant Professor - Department of Pathology and Laboratory Medicine (SMD)

Credentials

Education

PhD | University of Rochester School of Medicine & Dentistry. Biochemistry. 2009

MS | University of Rochester School of Medicine & Dentistry. Biochemistry. 2006

BS | University of Rochester. Biochemistry. 2003

Research

Chad Galloway is currently the Technical Manager of the Electron Microscopy Shared Resource Lab (EM-SRL). In his over 10 years of academic research experience at the University his research has encompassed diverse subject matter with a central focus of metabolic disease. Dr. Galloway brings to the EM-SRL expertise in the description and analysis of mitochondrial morphology and its reciprocal relationship with cellular bioenergetics, including the ultrastructural changes in mitochondria often perturbed by metabolic disease. During training in the Departments of Biochemistry, Anesthesiology and Pharmacology and Physiology at the University of Rochester he developed a broad background in biochemistry and cellular/molecular biology with a prevailing theme of metabolic disease including, diabetes, non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease. Studies of the pathophysiology of these diseases lead to extensive work describing the relationship between mitochondrial morphology and reactive oxygen species production and the resultant oxidative injury in metabolic flux relating pathologies, observed by microscopy, to biochemical endpoints. His studies have utilized immortal and primary cell culture systems for mechanistic dissection of these processes with transition to animal models, including transgenic mouse models, to study systemic effects with a focus on cardiac and hepatic tissues. In the Department of Ophthalmology Dr. Galloway continued his utilization of microscopy, both confocal and transmission electron microscopy (TEM), as a powerful tool while dissecting the pathophysiological changes in human induced pluripotent stem cells (hiPSC) from patients with inherited macular degenerative diseases. In a first of its kind study, "Drusen in patient-derived hiPSC-RPE models of macular dystrophies" in PNAS, hiPSC retinal pigment epithelial cells were reported to autonomously recapitulate macular degenerative patient phenotypes, including drusen-like deposits analyzed by TEM and confocal microscopy, in a cell culture system which produced a potent tool for the interrogation of the underlying disease mechanisms.
Working in the EM-SRL his focus will be relating the structural changes, from the comprehensive perspective of light and fluorescence microscopy to the finite details of subcellular ultrastructure utilizing scanning and transmission electron microscopy (SEM & TEM), to their functional consequences in the pathophysiology of human disease and models thereof. In the EM-SRL Dr. Galloway works with individual users to streamline and optimize sample procurement and processing utilizing his basic science research experience to develop novel methodologies and visualize the proverbial "needle in the haystack" ultrastructural changes and organelles by electron microscopy. In this guise, he also works to advance the technology and instrumentation available for investigators in the EM-SRL while continuing basic research interests in human Staphylococcus aureus infected bone TEM studies in collaboration with Dr. Irvin Oh in the Department of Orthopedics.

Publications

Journal Articles

The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.

Lee H, Lee TJ, Galloway CA, Zhi W, Xiao W, de Mesy Bentley KL, Sharma A, Teng Y, Sesaki H, Yoon Y

Nature communications.. 2023 October 2314 (1):6721. Epub 10/23/2023.

Arachnoid granulations are lymphatic conduits that communicate with bone marrow and dura-arachnoid stroma.

Shah T, Leurgans SE, Mehta RI, Yang J, Galloway CA, de Mesy Bentley KL, Schneider JA, Mehta RI

The Journal of experimental medicine.. 2023 February 6220 (2)Epub 12/05/2022.

Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer's disease.

Mestre H, Verma N, Greene TD, Lin LA, Ladron-de-Guevara A, Sweeney AM, Liu G, Thomas VK, Galloway CA, de Mesy Bentley KL, Nedergaard M, Mehta RI

Nature communications.. 2022 July 613 (1):3897. Epub 07/06/2022.

Efficacy of Bisphosphonate-Conjugated Sitafloxacin in a Murine Model of Osteomyelitis: Evidence of "Target & Release" Kinetics and Killing of Bacteria Within Canaliculi.

Ren Y, Xue T, Rainbolt J, Bentley KLM, Galloway CA, Liu Y, Cherian P, Neighbors J, Hofstee MI, Ebetino FH, Moriarty TF, Sun S, Schwarz EM, Xie C

Frontiers in cellular and infection microbiology.. 2022 12 :910970. Epub 06/24/2022.

3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration.

Manian KV, Galloway CA, Dalvi S, Emanuel AA, Mereness JA, Black W, Winschel L, Soto C, Li Y, Song Y, DeMaria W, Kumar A, Slukvin I, Schwartz MP, Murphy WL, Anand-Apte B, Chung M, Benoit DSW, Singh R

Cell stem cell.. 2021 May 628 (5):846-862.e8. Epub 03/29/2021.

3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration.

Manian KV, Galloway CA, Dalvi S, Emanuel AA, Mereness JA, Black W, Winschel L, Soto C, Li Y, Song Y, DeMaria W, Kumar A, Slukvin I, Schwartz MP, Murphy WL, Anand-Apte B, Chung M, Benoit DSW, Singh R

Cell stem cell.. 2021 May 628 (5):978. Epub 1900 01 01.

A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface.

Tang C, Han J, Dalvi S, Manian K, Winschel L, Volland S, Soto CA, Galloway CA, Spencer W, Roll M, Milliner C, Bonilha VL, Johnson TB, Latchney L, Weimer JM, Augustine EF, Mink JW, Gullapalli VK, Chung M, Williams DS, Singh R

Communications biology.. 2021 February 54 (1):161. Epub 02/05/2021.

Distinct vasculotropic versus osteotropic features of S. agalactiae versus S. aureus implant-associated bone infection in mice.

Masters EA, Hao SP, Mark Kenney H, Morita Y, Galloway CA, de Mesy Bentley KL, Ricciardi BF, Boyce BF, Schwarz EM, Oh I

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2021 February 39 (2):389-401. Epub 12/29/2020.

Emerging electron microscopy and 3D methodologies to interrogate Staphylococcus aureus osteomyelitis in murine models.

de Mesy Bentley KL, Galloway CA, Muthukrishnan G, Echternacht SR, Masters EA, Zeiter S, Schwarz EM, Leckenby JI

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2021 February 39 (2):376-388. Epub 01/13/2021.

Cell Wall Biosynthesis Modulates Bone Invasion and Osteomyelitis Pathogenesis.

Masters EA, Muthukrishnan G, Ho L, Gill AL, de Mesy Bentley KL, Galloway CA, McGrath JL, Awad HA, Gill SR, Schwarz EM

Frontiers in microbiology.. 2021 12 :723498. Epub 08/16/2021.

Identification of Penicillin Binding Protein 4 (PBP4) as a critical factor for Staphylococcus aureus bone invasion during osteomyelitis in mice.

Masters EA, de Mesy Bentley KL, Gill AL, Hao SP, Galloway CA, Salminen AT, Guy DR, McGrath JL, Awad HA, Gill SR, Schwarz EM

PLoS pathogens.. 2020 October 16 (10):e1008988. Epub 10/22/2020.

Environmental stress impairs photoreceptor outer segment (POS) phagocytosis and degradation and induces autofluorescent material accumulation in hiPSC-RPE cells.

Dalvi S, Galloway CA, Winschel L, Hashim A, Soto C, Tang C, MacDonald LA, Singh R

Cell death discovery.. 2019 5 :96. Epub 05/16/2019.

Pluripotent Stem Cells to Model Degenerative Retinal Diseases: The RPE Perspective.

Dalvi S, Galloway CA, Singh R

Advances in experimental medicine and biology.. 2019 1186 :1-31. Epub 1900 01 01.

Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin.

Galloway CA, Dalvi S, Shadforth AMA, Suzuki S, Wilson M, Kuai D, Hashim A, MacDonald LA, Gamm DM, Harkin DG, Singh R

Investigative ophthalmology & visual science.. 2018 June 159 (7):2792-2800. Epub 1900 01 01.

Drusen in patient-derived hiPSC-RPE models of macular dystrophies.

Galloway CA, Dalvi S, Hung SSC, MacDonald LA, Latchney LR, Wong RCB, Guymer RH, Mackey DA, Williams DS, Chung MM, Gamm DM, Pébay A, Hewitt AW, Singh R

Proceedings of the National Academy of Sciences of the United States of America.. 2017 September 26114 (39):E8214-E8223. Epub 09/06/2017.

Mitochondrial dynamics in diabetic cardiomyopathy.

Galloway CA, Yoon Y

Antioxidants & redox signaling.. 2015 June 1022 (17):1545-62. Epub 04/13/2015.

Decreasing mitochondrial fission alleviates hepatic steatosis in a murine model of nonalcoholic fatty liver disease.

Galloway CA, Lee H, Brookes PS, Yoon Y

American journal of physiology. Gastrointestinal and liver physiology.. 2014 September 15307 (6):G632-41. Epub 07/31/2014.

Mitochondrial morphology in metabolic diseases.

Galloway CA, Yoon Y

Antioxidants & redox signaling.. 2013 August 119 (4):415-30. Epub 08/27/2012.

Mitochondrial morphology-emerging role in bioenergetics.

Galloway CA, Lee H, Yoon Y