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Nancy Wang, Ph.D.

Nancy Wang, Ph.D.

About Me

Director of the Cytogenetics Laboratory since 1986, Dr. Wang has participated in the rapid advances in the field of cytogenetics. She has assisted in establishing and updating cytogenetics laboratories in the U.S. and in the Republic of China. She is currently the pathology participant in an NIH Cen...
Director of the Cytogenetics Laboratory since 1986, Dr. Wang has participated in the rapid advances in the field of cytogenetics. She has assisted in establishing and updating cytogenetics laboratories in the U.S. and in the Republic of China. She is currently the pathology participant in an NIH Center grant studying biophysical assessment and risk management after irradiation.

Faculty Appointments

Professor Emeritus - Department of Pathology and Laboratory Medicine (SMD)

Credentials

Education

PhD | Univ Minnesota-Twin City Cmp. Genetics. 1978

MS | Univ Minnesota-Twin City Cmp. Genetics. 1968

BS | Taiwan - Non-Medical School. Botany. 1966

Awards

Editorial Board. 2009

President, Association of Chinese Geneticists in America. 2000 - 2001

America's Outstanding Women of the 80's-America's Who's Who. 1989

Tenured Associate Professorship, Dept of Biochemistry. 1986

Charles E. Culpepper Fellowship. 1985 - 1986

Appointed to mediate the Cancer and Developmental Genetics Session. 1984

Research

Microcell-mediated transfer of chromosome 11 into ovarian carcinoma cell line, SKOV-3 results in suppression of tumorigenicity in scid/scid mice providing evidence from functional aspect that chromosome 11 carries ovarian tumor suppressor gene(s).

To identify the differentially expressed tra...
Microcell-mediated transfer of chromosome 11 into ovarian carcinoma cell line, SKOV-3 results in suppression of tumorigenicity in scid/scid mice providing evidence from functional aspect that chromosome 11 carries ovarian tumor suppressor gene(s).

To identify the differentially expressed transcripts associated with suppression of tumorigenicity, cDNA from the tumorigenic clones were subtracted from the non-tumorigenic clones which were then either cloned, sequenced and searched in GenBank, or analyzed by Gene Discovery Array Screening (GDAS). A cDNA transcript corresponding to the gene Thy-1 located (11q23-24) was found to be exclusively expressed in the non-tumorigenic clones. Transfection of Thy-1-antisence to the non-tumorigenic clone, 11(H)9-8, abolished the Thy-1 expression and restored tumorigenicity in scid/scid mice. We therefore, hypothesize that Thy-1 is a putative ovarian tumor suppressor gene (TSG).

To test this hypothesis, the following studies are ongoing: 1) To verify the tumor suppressing nature of Thy-1 and its specificity in fresh/frozen ovarian tumors by Northern blot, immunocytochemistry, and flow cytometry followed by statistical correlation study; 2) To define the effect of Thy-1 on in vivo growth and tumorigenicity by comparative xenographic study in scid/scid between 11(C)9-8-Thy-1-antisense and 11(C)9-8-null, as well as SKOV-3-Thy-1 and SKOV-3-null; 3) To identify the downstream genes associated with Thy-1 by comparative macroarray and microarray analysis combined with suppression subtractive hybridization on the DNA population between SKOV-3-Thy-1 and SKOV-3-null, as well as 11(C)9-8-Thy-1-antisense and 11(C)9-8-null; 4) To identify the sequential signaling pathway of Thy-1 using the in vitro doxycycline (dox) Thy-1 inducible system established in our laboratory. cDNA obtained from SKOV-3-Thy-1-inducible cells at various time post dox exposure will be analyzed and compared between adjacent time points by both macroarray and dual color microarray.

The accomplishment of this study cannot only verify the OVC tumor suppressing nature of Thy-1, but also let us understand the function of this important cell surface marker.

Publications

Journal Articles

Validating high-throughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: Benchmark against dicentric and CBMN assays in a mouse model

Chen YC,Tsai Y, Nowak I, Wang N, Hyrien O, Wilkins R, Ferrarotto C. Sun H, Dertinger SD.

Health Phys. 2010; 98(2): 218-2010.

Functional characterization of THY-1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma.

Lung HL, Cheung AK, Cheng Y, Kwong FM, Lo PH, Law EWL, Chua D, Zabarovsky ER, Wang N, Tsao SW, Stanbridge EJ, Lung ML.

Int J Cancer. 2009; .

Isodicentric Philadelphia chromosomes in imatinib mesylate (Gleevec)-resistant patients.

Szych CM, Liesveld JL, Iqbal MA, Li L, Siebert S, Asmus C, O'Malley J, Lee A, Wang N

Cancer genetics and cytogenetics.. 2007 April 15174 (2):132-7. Epub 1900 01 01.

Atlas of Hematological Diseases

McGraw-Hill Company. 2007; .

THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma.

Lung HL, Bangarusamy DK, Xie D, Cheung AK, Cheng Y, Kumaran MK, Miller L, Liu ET, Guan XY, Sham JS, Fang Y, Li L, Wang N, Protopopov AI, Zabarovsky ER, Tsao SW, Stanbridge EJ, Lung ML

Oncogene.. 2005 September 2924 (43):6525-32. Epub 1900 01 01.

THY-1 induction is associated with up-regulation of fibronectin and thrombospondin-1 in human ovarian cancer.

Abeysinghe HR, Li LQ, Guckert NL, Reeder J, Wang N

Cancer genetics and cytogenetics.. 2005 September 161 (2):151-8. Epub 1900 01 01.

Chromosome microdissection using conventional methods.

Wang, N; Li, L and Abeysinghe, HR.

Cell Biology: A Laboratory Handbook. 2005; Third Edition Volume 3 Chapter 3.

Differential gene expression between tumorigeni and non-tumorigenic hybrid clones of ovarian carcinoma cell line SKOV-3.

Xu, J; Li, L; Miller, R and Wang, N.

2005; (in preparation).

Human papillomavirus type 16 E6 and E7 cause polyploidy in human keratinocytes and up-regulation of G2-M-phase proteins.

Patel D, Incassati A, Wang N, McCance DJ

Cancer research.. 2004 February 1564 (4):1299-306. Epub 1900 01 01.

The role of the THY1 gene in human ovarian cancer suppression based on transfection studies.

Abeysinghe HR, Pollock SJ, Guckert NL, Veyberman Y, Keng P, Halterman M, Federoff HJ, Rosenblatt JP, Wang N

Cancer genetics and cytogenetics.. 2004 February 149 (1):1-10. Epub 1900 01 01.

THY1 expression is associated with tumor suppression of human ovarian cancer.

Abeysinghe HR, Cao Q, Xu J, Pollock S, Veyberman Y, Guckert NL, Keng P, Wang N

Cancer genetics and cytogenetics.. 2003 June 143 (2):125-32. Epub 1900 01 01.

Komrokji, R; Lancet, J; Felgar, R; Wang, N and Bennett, JM.

Burkitt's leukemia with precursor B-cell immunophenotype and atypical morphology (atypical Burkitt's leukemia/

Early gene activation in chronic leukemia B lymphocytes induced toward a plasma cell phenotype.

Segel, GB; Woodlock TJ; Xu, J; Li, L; Felgar, RE; Ryan, DH; Lichtman, MA and Wang, N.

Blood Cells, Molecules and Diseases. 2003; 30: 277-287.

Methodologies in cancer cytogenetics and molecular genetics.

Wang, N.

Am J Med Genetics. 2002; 115: 118-124.

Cytogenetics and molecular genetics of ovarian cancer.

Wang, N.

Am J Med Genetics. 2002; 115: 157-163.

Suppression of tumorigenicity in human ovarian carcinoma cell line SKOV-3 by microcell-mediated transfer of chromosome 11.

Cao Q, Abeysinghe H, Chow O, Xu J, Kaung H, Fong C, Keng P, Insel RA, Lee WM, Barrett JC, Wang N

Cancer genetics and cytogenetics.. 2001 September 129 (2):131-7. Epub 1900 01 01.

Amplification of C-MYC as the origin of the homogeneous staining region in ovarian carcinoma detected by micro-FISH.

Abeysinghe HR, Cedrone E, Tyan T, Xu J, Wang N

Cancer genetics and cytogenetics.. 1999 October 15114 (2):136-43. Epub 1900 01 01.

Trasplantation followed by requinimex.

Rowe, JM; Rapoport, AP; Ryan, DH; Nilsson, BI; Duert, RE; Packman, CH; Abboud, CN; DiPersio, JF; Linder, T and Wang, N.

Bone Marrow Trasplantation. 1999; 24(10): 1057-1063.

Prenatal identification of de novo marker chromosomes using micro-FISH approach.

Xu J, Fong CT, Cedrone E, Sullivan J, Wang N

Clinical genetics.. 1998 June 53 (6):490-6. Epub 1900 01 01.

Loss of heterozygosity at chromosome segment Xq25-26.1 in advanced human ovarian carcinomas.

Choi C, Cho S, Horikawa I, Berchuck A, Wang N, Cedrone E, Jhung SW, Lee JB, Kerr J, Chenevix-Trench G, Kim S, Barrett JC, Koi M

Genes, chromosomes & cancer.. 1997 November 20 (3):234-42. Epub 1900 01 01.

Detection of 11q13 amplification as the origin of homogeneous staining region in small cell lung cancer by chromosome microdissection.

Xu, J; Tyan, T; Cedrone, E; Swaragi, N and Wang, N.

Gene Chromosome Cancer. 1996; 17: 172-178.

The characterization of chromosomal rearrangements by a combined micro-FISH approach in a patient with myelodysplastic syndrome.

Xu J, Cedrone E, Roberts M, Wu G, Gershagen S, Wang N

Cancer genetics and cytogenetics.. 1995 September 83 (2):105-10. Epub 1900 01 01.

The clonal nature of mediastinal germ cell tumors and acute myelogenous leukemia. A case report and review of the literature.

Woodruff K, Wang N, May W, Adrone E, Denny C, Feig SA

Cancer genetics and cytogenetics.. 1995 January 79 (1):25-31. Epub 1900 01 01.

B-cell acute lymphoblastic leukemia with L1 morphology and coexistence of t(1;19) and t(14;18) chromosome translocations.

Kouides PA, Phatak PD, Wang N, Bennett JM

Cancer genetics and cytogenetics.. 1994 November 78 (1):23-7. Epub 1900 01 01.

Capping of bcr-abl antisense oligonucleotides enhances antiproliferative activity against chronic myeloid leukemia cell lines.

Thomas M, Kosciolek B, Wang N, Rowley P

Leukemia research.. 1994 June 18 (6):401-8. Epub 1900 01 01.

Identification of chromosomal structural alterations in human ovarian carcinoma cells using combined GTG-banding and repetitive fluorescence in situ hybridization (FISH).

Xu J, Wang N

Cancer genetics and cytogenetics.. 1994 May 74 (1):1-7. Epub 1900 01 01.

An infant with trisomy 9 mosaicism presenting as a complete trisomy 9 by amniocentesis.

Sherer DM, Wang N, Thompson HO, Peterson JC, Miller ME, Metlay LA, Abramowicz JS

Prenatal diagnosis.. 1992 January 12 (1):31-7. Epub 1900 01 01.

Differential evolution in chromosomal composition during multiple cycles of subcutaneous and intravenous metastasis.

Wang N, Youngblom J, Janatipour M, Shoffner RN

Cancer genetics and cytogenetics.. 1991 July 1554 (2):205-14. Epub 1900 01 01.

Prenatal diagnosis and subsequent management of a fetus with a 46,XY,r(4)(p15;q35) karyotype.

Sherer, DM; Shah, YG; Wang, N; Metlay, LA and Woods, JR.

Am J Perinatology. 1991; 8: 53-55.

X chromosome: Inactivation and reactivation. In: eukaryotic chromosome structural and functional aspects.

Wang, N.

Ed RC Sobti and G Obe. 1991; .

Interpretation of bone marrow and lymph node karyotyping: In: Hematology; basic principles and practice.

Wang, N.

Churchill Livingstone Publishing Company. 1991; .

Effects of ionizing radiation on human papillomavirus immortalized human bronchial cells. In: Neoplastic transformation in human cell culture: mechanism of carcinogenesis.

Willey, JC; Greene, J; Bressoud, A; Cerutti, P; Hez, T; Wang, N; Maillie, D; Cox, C and Miles, E.

Ed. Johng S. Rhim and Anatoly Dritschilo. Humana Press. 1991; : 113-118.

Transposition of the oncogene ets-1 in t(11;19) translocation in acute leukemia.

Wang N, Cedrone E, Dry J, Skuse GR, Schwartz C, Terryberry S

Cancer genetics and cytogenetics.. 1990 December 50 (2):199-205. Epub 1900 01 01.

Two identical active X chromosomes in human mammary carcinoma cells.

Wang N, Cedrone E, Skuse GR, Insel R, Dry J

Cancer genetics and cytogenetics.. 1990 June 46 (2):271-80. Epub 1900 01 01.

Expression of a specific protein in spontaneously metastatic fibrosarcoma cell lines and its enhanced synthesis by growth on laminin or fibronectin.

Youngblom JH, Wang N, McCarthy JB, Janatipour M, Wang T, Sheppard JR, Panter SS

Clinical & experimental metastasis.. 1990 8 (1):47-58. Epub 1900 01 01.

Chromosomal evolution in the progression and metastasis of human malignant melanoma. A multiple lesion study.

Pedersen MI, Wang N

Cancer genetics and cytogenetics.. 1989 September 41 (2):185-201. Epub 1900 01 01.

Clinical evaluation of a DNA probe assay for the Philadelphia (Ph1) translocation in chronic myelogenous leukemia.

Blennerhassett GT, Furth ME, Anderson A, Burns JP, Chaganti RS, Blick M, Talpaz M, Dev VG, Chan LC, Wiedemann LM

Leukemia.. 1988 October 2 (10):648-57. Epub 1900 01 01.

Nonrandom chromosomal aberrations and clonal chromosomal evolution in acute leukemia associated with Down's syndrome.

Wang N, Leung J, Warrier RP, Schorin M, Kirkpatrick D, Nowak MJ, Strand R

Cancer genetics and cytogenetics.. 1987 September 28 (1):155-62. Epub 1900 01 01.

Characterization of two human cell lines (TK-10, TK-164) of renal cell cancer.

Bear A, Clayman RV, Elbers J, Limas C, Wang N, Stone K, Gebhard R, Prigge W, Palmer J

Cancer research.. 1987 July 1547 (14):3856-62. Epub 1900 01 01.

Prenatal diagnosis of a 46,XX,inv(12)pat/47,XX,i(Xq),inv(12)pat.

Varela M, Wang N, Cerrillo M

Human genetics.. 1987 January 75 (1):93-4. Epub 1900 01 01.

Aberrant immunoglobulin and C-impage gene arrangement in patients with nonmalignant cryoglobulinemia.

Perl, A; Wang, N; William, JM; Hunt, MJ and Abraham GN.

J Immunology. 1987; 139: 3512-3520.

Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma.

Rangan SR, Martin LN, Bozelka BE, Wang N, Gormus BJ

International journal of cancer.. 1986 September 1538 (3):425-32. Epub 1900 01 01.

Immunological heterogeneity in human melanoma: immunogenic alloantigen expression in autologous host.

Hornung MO, Krementz ET, Sullivan KA, Muchmore JH, Prats I, Wang N, Strand R

Cancer research.. 1986 July 46 (7):3704-10. Epub 1900 01 01.

Alterations in the X chromosome replication pattern induced by 5-azacytidine in a human tumor line.

Gregory P, Wang N

Cancer genetics and cytogenetics.. 1986 February 1520 (3-4):263-7. Epub 1900 01 01.

Nonrandom chromosome structural aberrations and oncogene loci in human malignant melanoma.

Pedersen MI, Bennett JW, Wang N

Cancer genetics and cytogenetics.. 1986 February 120 (1-2):11-27. Epub 1900 01 01.

Analysis of sister chromatic exchange in Fra(X) individuals.

Gregory, P; Howard-Peebles, P and Wang N.

Am J Medical Genetics. 1986; 23: 563-566.

A practical metaphase marker of the inactive X-chromosome.

Van Dyke, DL; Flejter, WL; Worsham, MJ; Robinson, JR; Higgins, JV; Hen, HM; Weiss, L and Wang, N.

Am H Hum Genet. 1986; 39: 88-95.

Multiple oncogene alterations in human malignant melanoma.

Pedersen, M and Wang N.

Am J Human Genet. 1986; 39: 37.

Familial osteosarcoma associated with 13;14 chromosomal rearrangement.

Gilman PA, Wang N, Fan SF, Reede J, Khan A, Leventhal BG

Cancer genetics and cytogenetics.. 1985 June 17 (2):123-32. Epub 1900 01 01.

Changes in the X-chromosome replication pattern induced by 5-azacytidine in a patient with Klinefelter syndrome.

Gregory, P; Green, C; Shapira, B and Wang N.

Cytogenetic Cell Genetics. 1985; 39: 234-236.

Sister chromatid exchanges in adult epileptic patients on phenytoin therapy.

Schauman, B; Wang, N; Johnson, S and Van Brunt S.

Environ Mutagenesis. 1985; 7: 711-714.

Studies of lymph nodes from patients with classical hemophilia.

Andes WA, deShazo RD, Reed RJ, Harkin JC, Wang NN

Blood.. 1984 October 64 (4):768-73. Epub 1900 01 01.

Involvement of band 3p14 in t(3;8) hereditary renal carcinoma.

Wang N, Perkins KL

Cancer genetics and cytogenetics.. 1984 April 11 (4):479-81. Epub 1900 01 01.

The effect of immune pressure on pulmonary metastasis of a methylcholanthrene induced fibrosarcoma and three of its clones.

Yu, S; Wang, N and McKhann, C.

Surg Oncology. 1984; 27: 51-58.

Proteolytic and metastatic activities of clones derived from a methylcholanthrene induced fibrosarcoma.

McLaughlin, M; Liener, IE and Wang N.

Cin Expl Metastasis. 1983; 1: 359-371.

Chromosomal replication asynchrony of a human breast carcinoma cell line. I. studied by continuous BrdU incorporation and G-banding analysis.

Wang N, Trend B, Kaung HL, Wang T

Cancer genetics and cytogenetics.. 1982 October 7 (2):173-80. Epub 1900 01 01.

The induction of chromosomal structural changes in male chickens by the alkylating agents: triethylene melamine and ethyl methanesulfonate.

Wang N, Shoffner RN, Otis JS, Cheng KM

Mutation research.. 1982 September 96 (1):53-66. Epub 1900 01 01.

Retinoblastoma and its association with a deletion in chromosome #13: a survey using high-resolution chromosome techniques.

Johnson MP, Ramsay N, Cervenka J, Wang N

Cancer genetics and cytogenetics.. 1982 May 6 (1):29-37. Epub 1900 01 01.

Cyclic biological expression in mouse mammary tumors.

Kiang DT, King M, Zhang HJ, Kennedy BJ, Wang N

Science.. 1982 April 2216 (4541):68-70. Epub 1900 01 01.

Regressing atypical histiocytosis: a cutaneous proliferation of atypical neoplastic histiocytes with unexpectedly indolent biologic behavior.

Flynn KJ, Dehner LP, Gajl-Peczalska KJ, Dahl MV, Ramsay N, Wang N

Cancer.. 1982 March 149 (5):959-70. Epub 1900 01 01.

Characterization of high- and low-metastatic clones derived from a methylcholanthrene-induced murine fibrosarcoma.

Wang N, Yu SH, Liener IE, Hebbel RP, Eaton JW, McKhann CF

Cancer research.. 1982 March 42 (3):1046-51. Epub 1900 01 01.

Regressing atypical histiocytosis: benign or malignant?

Flynn KJ, Dehner LP, Gajl-Peczalska KJ, Dahl MV, Ramsay N, Wang N

Minnesota medicine.. 1982 February 65 (2):73-6. Epub 1900 01 01.

Recombinant inversion chromosomes in phenotypically normal chickens.

Bitgood JJ, Shoffner RN, Otis JS, Wang N

Science.. 1982 January 22215 (4531):409-11. Epub 1900 01 01.

Hereditary renal carcinoma and chromosomal defects.

Kantor, AF; Blattner, WA; Blot, WJ; Frumeni, JF; McLaughlin, JK; Schuman, LH; Lindquist, LL and Wang N.

New Eng J Med. 1982; 307: 140.

Differential induction of sister chromatid exchange by chemical carcinogens in lymphocytes cultured from patients with solid tumors.

Wiencke JK, Vosika J, Johnson P, Wang N, Garry VF

Pharmacology.. 1982 24 (2):67-73. Epub 1900 01 01.

Cytogenetic evidence for premeiotic transformation of human testicular cancers.

Wang N, Perkins KL, Bronson DL, Fraley EE

Cancer research.. 1981 June 41 (6):2135-40. Epub 1900 01 01.

The induction and detection of diploid spermatozoa in Gallus domesticus.

Wang N, Sheppard JR, Wang T, Shoffner RN

Mutation research.. 1980 December 73 (2):279-90. Epub 1900 01 01.

Nonrandom abnormalities in chromosome 1 in human testicular cancers.

Wang N, Trend B, Bronson DL, Fraley EE

Cancer research.. 1980 March 40 (3):796-802. Epub 1900 01 01.

Induction of heteroploidy in gallus domesticus.

Wang, N and Shoffner, RN.

Mutation Res. 1980; 69: 262-273.

Cytogenetic evidence for the absence of an inactivated X-chromosome in human female (XX) breast carcinoma cell line.

Camargo, M and Wang, N.

Human Genet. 1980; 55: 81-85.

Chromosomal homology between the Ross's and the Emperor goose.

Shoffner, RN; Wang, N; Lee, F; King, R and Otis, JJ.

Heredity. 1979; 70: 395-400.

Trypsin G- and C-banding for interchange analysis and sex identification in the chicken.

Wang N, Shoffner RN

Chromosoma. 1974 47 (1):61-9. Epub 1900 01 01.

THY-1 tumor suppressive function mediated though CAPM1 and CRYAB in nasopharyngeal carcinoma.

Lung HL; Cheung AKL; Cheng Y; Kwong FM; Lo PHY; Law EWL; Chua D; Zabarousky ER; Wang N; Tsao SW; Stanbridge E; Lung ML.

Cancer Research. .