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David A. Dean, Ph.D.

David A. Dean, Ph.D.

Contact

Office (585) 276-3933
Office (585) 756-7780
Administrative (585) 275-5948

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About Me

Faculty Appointments

Professor - Department of Pediatrics, Neonatology (SMD)

Credentials

Post-doctoral Training & Residency

University of California, Los Angeles Postdoctoral Fellow Virology 1990 - 1994

Education

PhD | University of California at Berkeley. Microbiology. 1990

PhD | University of California, Berkeley. Microbiology. 1990

BA | University of California at San Diego. Biochemistry. 1985

Awards

Keynote Speaker. 2019

Chair. 2014

Keynote Speaker. 2013

Co-Chair. 2012

Ruth A. Lawrence Academic Faculty Service Award in Research. 2011

Mentoring the Mentors. 2006

Sandler Program for Asthma Research Senior Investigator. 2005

NIH Individual Postdoctoral Fellowship (NRSA). 1991 - 1994

President's Undergraduate Scholarship. 1984

Research

Despite all of the hype regarding gene therapy, at present, gene therapy is a dream due to insufficient levels of gene transfer and expression at desired sites. One way to increase gene expression is to target more DNA to the cell nucleus. Since the nucleus is the site of transcription, without th...
Despite all of the hype regarding gene therapy, at present, gene therapy is a dream due to insufficient levels of gene transfer and expression at desired sites. One way to increase gene expression is to target more DNA to the cell nucleus. Since the nucleus is the site of transcription, without the movement of plasmids through the cytoplasm, translocation into the nucleus, and localization to the appropriate subnuclear domain, no gene expression, or "gene therapy" can take place. Ongoing projects in my laboratory are studying the mechanisms of cytoplasmic trafficking of plasmids along the cytoskeleton, plasmid nuclear entry, subnuclear organization, and exploiting what we learn to improve gene therapy.


We have shown that plasmids are able to enter the nucleus in a sequence-specific manner that appears to be mediated by transcription factor binding. My lab is interested in identifying the proteins required for this activity and the regulation of their nuclear import. Based on our model, we have created cell-specific plasmids by incorporating DNA sequences that bind to cell-specific transcription factors. At present, we have examples of smooth muscle, alveolar epithelial cell, and endothelial cell-targeting vectors that we hope to use for the treatment of a number of pulmonary diseases, and we are working to expand our repertoire to selectively target expression to any desired cell or tissue.


We also are developing methods for extracellular delivery of non-viral vectors in animal models for disease. Using electroporation, we have obtained very high levels of gene expression in the vasculature and lungs of animals. We have used this approach to prevent and/or cure existing disease in models of acute lung injury and asthma in mice and rats. Our next goal is to optimize these approaches in more relevant preclinical models to move toward clinical application.

Publications

Journal Articles

MTOR maintains endothelial cell integrity to limit lung vascular injury.

Millar MW, Najar RA, Slavin SA, Shadab M, Tahir I, Mahamed Z, Lin X, Abe JI, Wright TW, Dean DA, Fazal F, Rahman A

The Journal of biological chemistry.. 2024 November 5 :107952. Epub 11/05/2024.

Occludin Is Essential to Maintain Normal Alveolar Barrier Integrity and Its Protective Role During ARDS Progression.

Lin X, Bai H, Barravecchia M, Norman R, Schiralli Lester GM, Kottmann RM, Leonard A, Rahman A, Young JL, Dean DA

International journal of molecular sciences.. 2024 October 2925 (21)Epub 10/29/2024.

Impact of Peptide Sequence on Functional siRNA Delivery and Gene Knockdown with Cyclic Amphipathic Peptide Delivery Agents.

Jagrosse ML, Baliga UK, Jones CW, Russell JJ, García CI, Najar RA, Rahman A, Dean DA, Nilsson BL

Molecular pharmaceutics.. 2023 December 420 (12):6090-6103. Epub 11/14/2023.

Spleen Tyrosine Kinase phosphorylates VE-cadherin to cause endothelial barrier disruption in acute lung injury.

Shadab M, Slavin SA, Mahamed Z, Millar MW, Najar RA, Leonard A, Pietropaoli A, Dean DA, Fazal F, Rahman A

The Journal of biological chemistry.. 2023 December 299 (12):105408. Epub 10/28/2023.

Multiple conductance states of lipid pores during Voltage-Clamp electroporation.

Gurunian A, Dean DA

Bioelectrochemistry.. 2023 June 151 :108396. Epub 02/11/2023.

Upregulation of alveolar fluid clearance is not sufficient for Na,K-ATPase ? subunit-mediated gene therapy of LPS-induced acute lung injury in mice.

Liu J, Schiralli-Lester GM, Norman R, Dean DA

Scientific reports.. 2023 April 2613 (1):6792. Epub 04/26/2023.

Modeling and simulation of current-clamp electroporation.

Gurunian A, Dean DA

Bioelectrochemistry.. 2022 October 147 :108162. Epub 05/19/2022.

Gene transfer of MRCK? rescues lipopolysaccharide-induced acute lung injury by restoring alveolar capillary barrier function.

Liu J, Dean DA

Scientific reports.. 2021 October 2111 (1):20862. Epub 10/21/2021.

The Na+, K+-ATPase ?1 subunit regulates epithelial tight junctions via MRCK?.

Bai H, Zhou R, Barravecchia M, Norman R, Friedman AE, Yu D, Lin X, Young JL, Dean DA

JCI insight.. 2021 February 226 (4)Epub 02/22/2021.

Pulmonary gene delivery-Realities and possibilities.

Baliga UK, Dean DA

Experimental biology and medicine. 2021 February 246 (3):260-274. Epub 11/12/2020.

Changes in lung immune cell infiltrates after electric field treatment in mice.

Eliseeva SI, Knowlden ZA, Lester GM, Dean DA, Georas SN, Chapman TJ

Scientific reports.. 2021 January 1411 (1):1453. Epub 01/14/2021.

Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.

Welch JJ, Dean DA, Nilsson BL

Methods in molecular biology.. 2021 2208 :49-67. Epub 1900 01 01.

Gene Therapy for Acute Respiratory Distress Syndrome.

Liu J, Dean DA

Frontiers in physiology.. 2021 12 :786255. Epub 01/17/2022.

Disruption of normal patterns of FOXF1 expression in a lethal disorder of lung development.

Steiner LA, Getman M, Schiralli Lester GM, Iqbal MA, Katzman P, Szafranski P, Stankiewicz P, Bhattacharya S, Mariani T, Pryhuber G, Lin X, Young JL, Dean DA, Scheible K

Journal of medical genetics.. 2020 May 57 (5):296-300. Epub 10/29/2019.

In vivo rescue of recombinant Zika virus from an infectious cDNA clone and its implications in vaccine development.

Ávila-Pérez G, Nogales A, Park JG, Vasquez DM, Dean DA, Barravecchia M, Perez DR, Almazán F, Martínez-Sobrido L

Scientific reports.. 2020 January 1610 (1):512. Epub 01/16/2020.

RNAi therapeutic strategies for acute respiratory distress syndrome.

Jagrosse ML, Dean DA, Rahman A, Nilsson BL

Translational research : the journal of laboratory and clinical medicine.. 2019 December 214 :30-49. Epub 07/27/2019.

Critical role of autophagy regulator Beclin1 in endothelial cell inflammation and barrier disruption.

Leonard A, Millar MW, Slavin SA, Bijli KM, Dionisio Santos, Dean DA, Fazal F, Rahman A

Cellular signalling.. 2019 September 61 :120-129. Epub 05/01/2019.

Cytoplasmic transport and nuclear import of plasmid DNA.

Bai H, Schiralli Lester GM, Petishnok LC, Dean DA

Bioscience reports.. 2017 December 2237 (6)Epub 11/29/2017.

Featured Article: Electroporation-mediated gene delivery of surfactant protein B (SP-B) restores expression and improves survival in mouse model of SP-B deficiency.

Barnett RC, Lin X, Barravecchia M, Norman RA, de Mesy Bentley KL, Fazal F, Young JL, Dean DA

Experimental biology and medicine. 2017 July 242 (13):1345-1354. Epub 06/05/2017.

Leptomycin B alters the subcellular distribution of CRM1 (Exportin 1).

Rahmani K, Dean DA

Biochemical and biophysical research communications.. 2017 June 24488 (2):253-258. Epub 04/12/2017.

Identification of differentially regulated genes in human patent ductus arteriosus.

Parikh P, Bai H, Swartz MF, Alfieris GM, Dean DA

Experimental biology and medicine. 2016 December 241 (18):2112-2118. Epub 07/28/2016.

Identification of an alveolar type I epithelial cell-specific DNA nuclear import sequence for gene delivery.

Gottfried L, Lin X, Barravecchia M, Dean DA

Gene therapy.. 2016 October 23 (10):734-742. Epub 07/01/2016.

Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides.

Welch JJ, Swanekamp RJ, King C, Dean DA, Nilsson BL

ACS medicinal chemistry letters.. 2016 June 97 (6):584-9. Epub 03/30/2016.

?1-Na(+),K(+)-ATPase gene therapy upregulates tight junctions to rescue lipopolysaccharide-induced acute lung injury.

Lin X, Barravecchia M, Kothari P, Young JL, Dean DA

Gene therapy.. 2016 June 23 (6):489-99. Epub 03/17/2016.

DNA Targeting Sequence Improves Magnetic Nanoparticle-Based Plasmid DNA Transfection Efficiency in Model Neurons.

Vernon MM, Dean DA, Dobson J

International journal of molecular sciences.. 2015 August 1716 (8):19369-86. Epub 08/17/2015.

High expression of carbonic anhydrase IX is significantly associated with glandular lesions in gastroesophageal junction and with tumorigenesis markers BMI1, MCM4 and MCM7.

Huber AR, Tan D, Sun J, Dean D, Wu T, Zhou Z

BMC gastroenterology.. 2015 July 915 :80. Epub 07/09/2015.

Electroporation-mediated gene delivery of Na+,K+ -ATPase, and ENaC subunits to the lung attenuates acute respiratory distress syndrome in a two-hit porcine model.

Emr BM, Roy S, Kollisch-Singule M, Gatto LA, Barravecchia M, Lin X, Young JL, Wang G, Liu J, Satalin J, Snyder K, Nieman GF, Dean DA

Shock.. 2015 January 43 (1):16-23. Epub 1900 01 01.

Electroporation-mediated gene delivery.

Young JL, Dean DA

Advances in genetics.. 2015 89 :49-88. Epub 12/11/2014.

Electroporation-mediated gene delivery to the lungs.

Young JL, Barravecchia MS, Dean DA

Methods in molecular biology.. 2014 1121 :189-204. Epub 1900 01 01.

Airway pressure release ventilation prevents ventilator-induced lung injury in normal lungs.

Emr B, Gatto LA, Roy S, Satalin J, Ghosh A, Snyder K, Andrews P, Habashi N, Marx W, Ge L, Wang G, Dean DA, Vodovotz Y, Nieman G

JAMA surgery.. 2013 November 148 (11):1005-12. Epub 1900 01 01.

Cell-specific targeting strategies for electroporation-mediated gene delivery in cells and animals.

Dean DA

The Journal of membrane biology.. 2013 October 246 (10):737-44. Epub 03/24/2013.

Preemptive application of airway pressure release ventilation prevents development of acute respiratory distress syndrome in a rat traumatic hemorrhagic shock model.

Roy SK, Emr B, Sadowitz B, Gatto LA, Ghosh A, Satalin JM, Snyder KP, Ge L, Wang G, Marx W, Dean D, Andrews P, Singh A, Scalea T, Habashi N, Nieman GF

Shock.. 2013 September 40 (3):210-6. Epub 1900 01 01.

Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules.

Badding MA, Dean DA

Gene therapy.. 2013 June 20 (6):616-24. Epub 09/27/2012.

Proteomic and functional analyses of protein-DNA complexes during gene transfer.

Badding MA, Lapek JD, Friedman AE, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2013 April 21 (4):775-85. Epub 11/20/2012.

Early airway pressure release ventilation prevents ARDS-a novel preventive approach to lung injury.

Roy S, Habashi N, Sadowitz B, Andrews P, Ge L, Wang G, Roy P, Ghosh A, Kuhn M, Satalin J, Gatto LA, Lin X, Dean DA, Vodovotz Y, Nieman G

Shock.. 2013 January 39 (1):28-38. Epub 1900 01 01.

Insertion of a nuclear factor kappa B DNA nuclear-targeting sequence potentiates suicide gene therapy efficacy in lung cancer cell lines.

Cramer F, Christensen CL, Poulsen TT, Badding MA, Dean DA, Poulsen HS

Cancer gene therapy.. 2012 October 19 (10):675-83. Epub 08/17/2012.

Neonatal oxygen increases sensitivity to influenza A virus infection in adult mice by suppressing epithelial expression of Ear1.

O'Reilly MA, Yee M, Buczynski BW, Vitiello PF, Keng PC, Welle SL, Finkelstein N, Dean DA, Lawrence BP

The American journal of pathology.. 2012 August 181 (2):441-51. Epub 06/05/2012.

Early stabilizing alveolar ventilation prevents acute respiratory distress syndrome: a novel timing-based ventilatory intervention to avert lung injury.

Roy S, Sadowitz B, Andrews P, Gatto LA, Marx W, Ge L, Wang G, Lin X, Dean DA, Kuhn M, Ghosh A, Satalin J, Snyder K, Vodovotz Y, Nieman G, Habashi N

The journal of trauma and acute care surgery.. 2012 August 73 (2):391-400. Epub 1900 01 01.

SIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in mice.

Yao H, Chung S, Hwang JW, Rajendrasozhan S, Sundar IK, Dean DA, McBurney MW, Guarente L, Gu W, Rönty M, Kinnula VL, Rahman I

The Journal of clinical investigation.. 2012 June 122 (6):2032-45. Epub 05/01/2012.

Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.

Badding MA, Vaughan EE, Dean DA

Gene therapy.. 2012 March 19 (3):338-46. Epub 06/30/2011.

Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.

Badding MA, Vaughan EE, Dean DA

Gene therapy.. 2012 March 19 (3):354. Epub 07/21/2011.

Gene therapy for ALI/ARDS.

Lin X, Dean DA

Critical care clinics.. 2011 July 27 (3):705-18. Epub 1900 01 01.

Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics.

Eldib M, Dean DA

Biotechnology and bioengineering.. 2011 February 108 (2):446-53. Epub 1900 01 01.

Use of Electroporation for Efficacious Gene Delivery to the Lungs

Dean, D. A., M. Barravecchia, B. Danziger, and X. Lin.

ECS Trans. 2011; 35: 167-177.

Epithelial ablation of Bcl-XL increases sensitivity to oxygen without disrupting lung development.

Staversky RJ, Vitiello PF, Yee M, Callahan LM, Dean DA, O'Reilly MA

American journal of respiratory cell and molecular biology.. 2010 September 43 (3):376-85. Epub 10/30/2009.

Electroporation- and mechanical ventilation-mediated gene transfer to the lung.

Kaufman CD, Geiger RC, Dean DA

Gene therapy.. 2010 September 17 (9):1098-104. Epub 04/29/2010.

The SP-C promoter facilitates alveolar type II epithelial cell-specific plasmid nuclear import and gene expression.

Degiulio JV, Kaufman CD, Dean DA

Gene therapy.. 2010 April 17 (4):541-9. Epub 01/07/2010.

Progress and prospects: nuclear import of nonviral vectors.

Lam AP, Dean DA

Gene therapy.. 2010 April 17 (4):439-47. Epub 03/04/2010.

Nephron-deficient Fvb mice develop rapidly progressive renal failure and heavy albuminuria involving excess glomerular GLUT1 and VEGF.

Wang Y, Heilig KO, Minto AW, Chen S, Xiang M, Dean DA, Geiger RC, Chang A, Pravtcheva DD, Schlimme M, Deb DK, Wang Y, Heilig CW

Laboratory investigation; a journal of technical methods and pathology.. 2010 January 90 (1):83-97. Epub 11/16/2009.

Identification of protein cofactors necessary for sequence-specific plasmid DNA nuclear import.

Miller AM, Munkonge FM, Alton EW, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2009 November 17 (11):1897-903. Epub 06/16/2009.

Neonatal oxygen adversely affects lung function in adult mice without altering surfactant composition or activity.

Yee M, Chess PR, McGrath-Morrow SA, Wang Z, Gelein R, Zhou R, Dean DA, Notter RH, O'Reilly MA

American journal of physiology. Lung cellular and molecular physiology.. 2009 October 297 (4):L641-9. Epub 07/17/2009.

Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear import.

Munkonge FM, Amin V, Hyde SC, Green AM, Pringle IA, Gill DR, Smith JW, Hooley RP, Xenariou S, Ward MA, Leeds N, Leung KY, Chan M, Hillery E, Geddes DM, Griesenbach U, Postel EH, Dean DA, Dunn MJ, Alton EW

The Journal of biological chemistry.. 2009 September 25284 (39):26978-87. Epub 07/28/2009.

Targeted G-protein inhibition as a novel approach to decrease vagal atrial fibrillation by selective parasympathetic attenuation.

Aistrup GL, Villuendas R, Ng J, Gilchrist A, Lynch TW, Gordon D, Cokic I, Mottl S, Zhou R, Dean DA, Wasserstrom JA, Goldberger JJ, Kadish AH, Arora R

Cardiovascular research.. 2009 August 183 (3):481-92. Epub 05/20/2009.

Tissue-specific and transcription factor-mediated nuclear entry of DNA.

Miller AM, Dean DA

Advanced drug delivery reviews.. 2009 July 261 (7-8):603-13. Epub 04/23/2009.

Designed multi-domain protein as a carrier of nucleic acids into cells.

Xavier J, Singh S, Dean DA, Rao NM, Gopal V

Journal of controlled release : official journal of the Controlled Release Society.. 2009 January 19133 (2):154-60. Epub 10/11/2008.

Tubulin acetylation and histone deacetylase 6 activity in the lung under cyclic load.

Geiger RC, Kaufman CD, Lam AP, Budinger GR, Dean DA

American journal of respiratory cell and molecular biology.. 2009 January 40 (1):76-82. Epub 07/17/2008.

Microtubule acetylation through HDAC6 inhibition results in increased transfection efficiency.

Vaughan EE, Geiger RC, Miller AM, Loh-Marley PL, Suzuki T, Miyata N, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2008 November 16 (11):1841-7. Epub 09/09/2008.

Cell-specific nuclear import of plasmid DNA in smooth muscle requires tissue-specific transcription factors and DNA sequences.

Miller AM, Dean DA

Gene therapy.. 2008 August 15 (15):1107-15. Epub 05/22/2008.

Smooth muscle-specific gene delivery in the vasculature based on restriction of DNA nuclear import.

Young JL, Zimmer WE, Dean DA

Experimental biology and medicine. 2008 July 233 (7):840-8. Epub 04/29/2008.

Cyclic stretch-induced nuclear localization of transcription factors results in increased nuclear targeting of plasmids in alveolar epithelial cells.

Lam AP, Dean DA

The journal of gene medicine.. 2008 June 10 (6):668-78. Epub 1900 01 01.

Electrical Impedance Spectroscopy Study of Biological Tissues.

Dean DA, Ramanathan T, Machado D, Sundararajan R

Journal of electrostatics.. 2008 March 66 (3-4):165-177. Epub 1900 01 01.

Quantifying Induced Electric Field Strengths During Gene Transfer to the Intact Rat Vasculature.

Capaccio, C.; Stoykov, N.S.; Sundarajan, R.; and D.A. Dean.

J Electrostatics. 2008; : (in press).

Electroporation-mediated gene delivery to the lungs.

Zhou R, Norton JE, Dean DA

Methods in molecular biology.. 2008 423 :233-47. Epub 1900 01 01.

Intranuclear trafficking of episomal DNA is transcription-dependent.

Gasiorowski JZ, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2007 December 15 (12):2132-9. Epub 07/31/2007.

Ambient particulate matter accelerates coagulation via an IL-6-dependent pathway.

Mutlu GM, Green D, Bellmeyer A, Baker CM, Burgess Z, Rajamannan N, Christman JW, Foiles N, Kamp DW, Ghio AJ, Chandel NS, Dean DA, Sznajder JI, Budinger GR

The Journal of clinical investigation.. 2007 October 117 (10):2952-61. Epub 1900 01 01.

Electroporation-mediated gene transfer of the Na+,K+ -ATPase rescues endotoxin-induced lung injury.

Mutlu GM, Machado-Aranda D, Norton JE, Bellmeyer A, Urich D, Zhou R, Dean DA

American journal of respiratory and critical care medicine.. 2007 September 15176 (6):582-90. Epub 06/07/2007.

Electroporation-mediated transfer of plasmids to the lung results in reduced TLR9 signaling and inflammation.

Zhou R, Norton JE, Zhang N, Dean DA

Gene therapy.. 2007 May 14 (9):775-80. Epub 03/08/2007.

Leptin resistance protects mice from hyperoxia-induced acute lung injury.

Bellmeyer A, Martino JM, Chandel NS, Scott Budinger GR, Dean DA, Mutlu GM

American journal of respiratory and critical care medicine.. 2007 March 15175 (6):587-94. Epub 12/21/2006.

Gene transfer of interleukin 10 to the murine cornea using electroporation.

Zhou R, Dean DA

Experimental biology and medicine. 2007 March 232 (3):362-9. Epub 1900 01 01.

Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.

Kopp K, Gasiorowski JZ, Chen D, Gilmore R, Norton JT, Wang C, Leary DJ, Chan EK, Dean DA, Huang S

Molecular biology of the cell.. 2007 February 18 (2):394-403. Epub 11/15/2006.

Quantifying Induced Electric Field Strengths During Gene Transfer to the Intact Rat Vasculature.

Capaccio, C., N. S. Stoykov, R. Sundararajan, and D. A. Dean.

IEEE Trans. Biomed. Eng. 2007; .

Intracellular trafficking of plasmids for gene therapy: mechanisms of cytoplasmic movement and nuclear import.

Vaughan EE, DeGiulio JV, Dean DA

Current gene therapy.. 2006 December 6 (6):671-681. Epub 1900 01 01.

Nondisruptive, sequence-specific coupling of fluorochromes to plasmid DNA.

Hillery E, Munkonge FM, Xenariou S, Dean DA, Alton EW

Analytical biochemistry.. 2006 May 15352 (2):169-75. Epub 03/09/2006.

Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer.

Geiger RC, Taylor W, Glucksberg MR, Dean DA

Gene therapy.. 2006 April 13 (8):725-31. Epub 1900 01 01.

Intracellular trafficking of plasmids during transfection is mediated by microtubules.

Vaughan EE, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2006 February 13 (2):422-8. Epub 11/21/2005.

Postmitotic nuclear retention of episomal plasmids is altered by DNA labeling and detection methods.

Gasiorowski JZ, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2005 September 12 (3):460-7. Epub 1900 01 01.

Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals.

Dean DA

American journal of physiology. Cell physiology.. 2005 August 289 (2):C233-45. Epub 1900 01 01.

Laminin-6 assembles into multimolecular fibrillar complexes with perlecan and participates in mechanical-signal transduction via a dystroglycan-dependent, integrin-independent mechanism.

Jones JC, Lane K, Hopkinson SB, Lecuona E, Geiger RC, Dean DA, Correa-Meyer E, Gonzales M, Campbell K, Sznajder JI, Budinger S

Journal of cell science.. 2005 June 15118 (Pt 12):2557-66. Epub 05/31/2005.

Nuclear entry of nonviral vectors.

Dean DA, Strong DD, Zimmer WE

Gene therapy.. 2005 June 12 (11):881-90. Epub 1900 01 01.

Gene transfer of the Na+,K+-ATPase beta1 subunit using electroporation increases lung liquid clearance.

Machado-Aranda D, Adir Y, Young JL, Briva A, Budinger GR, Yeldandi AV, Sznajder JI, Dean DA

American journal of respiratory and critical care medicine.. 2005 February 1171 (3):204-11. Epub 10/29/2004.

Effect of vinblastine on transfection: influence of cell types, cationic lipids and promoters.

Wang L, Dean DA, Macdonald RC

Current drug delivery.. 2005 January 2 (1):93-6. Epub 1900 01 01.

Intracellular trafficking of nucleic acids.

Zhou R, Geiger RC, Dean DA

Expert opinion on drug delivery.. 2004 November 1 (1):127-40. Epub 1900 01 01.

Hydroporation as the mechanism of hydrodynamic delivery.

Zhang G, Gao X, Song YK, Vollmer R, Stolz DB, Gasiorowski JZ, Dean DA, Liu D

Gene therapy.. 2004 April 11 (8):675-82. Epub 1900 01 01.

Electroporation of the vasculature and the lung.

Dean DA

DNA and cell biology.. 2003 December 22 (12):797-806. Epub 1900 01 01.

Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression.

Nunamaker EA, Zhang HY, Shirasawa Y, Benoit JN, Dean DA

American journal of physiology. Heart and circulatory physiology.. 2003 November 285 (5):H2240-7. Epub 07/24/2003.

Electroporation as a method for high-level nonviral gene transfer to the lung.

Dean DA, Machado-Aranda D, Blair-Parks K, Yeldandi AV, Young JL

Gene therapy.. 2003 September 10 (18):1608-15. Epub 1900 01 01.

Effect of a DNA nuclear targeting sequence on gene transfer and expression of plasmids in the intact vasculature.

Young JL, Benoit JN, Dean DA

Gene therapy.. 2003 August 10 (17):1465-70. Epub 1900 01 01.

Mechanisms of nuclear transport and interventions.

Gasiorowski JZ, Dean DA

Advanced drug delivery reviews.. 2003 June 1655 (6):703-16. Epub 1900 01 01.

Emerging significance of plasmid DNA nuclear import in gene therapy.

Munkonge FM, Dean DA, Hillery E, Griesenbach U, Alton EW

Advanced drug delivery reviews.. 2003 June 1655 (6):749-60. Epub 1900 01 01.

Modulation of protein kinase C (PKC)-mediated contraction and the possible role of PKC epsilon in rat mesenteric arteries.

Shirasawa Y, Rutland TJ, Young JL, Dean DA, Joseph BN

Frontiers in bioscience : a journal and virtual library.. 2003 May 18 :a133-8. Epub 05/01/2003.

The effects of cyclic stretch on gene transfer in alveolar epithelial cells.

Taylor W, Gokay KE, Capaccio C, Davis E, Glucksberg M, Dean DA

Molecular therapy : the journal of the American Society of Gene Therapy.. 2003 April 7 (4):542-9. Epub 1900 01 01.

Hyperoxia-induced apoptosis does not require mitochondrial reactive oxygen species and is regulated by Bcl-2 proteins.

Budinger GR, Tso M, McClintock DS, Dean DA, Sznajder JI, Chandel NS

The Journal of biological chemistry.. 2002 May 3277 (18):15654-60. Epub 02/27/2002.

Transforming growth factor-beta induction of smooth muscle cell phenotpye requires transcriptional and post-transcriptional control of serum response factor.

Hirschi KK, Lai L, Belaguli NS, Dean DA, Schwartz RJ, Zimmer WE

The Journal of biological chemistry.. 2002 February 22277 (8):6287-95. Epub 12/11/2001.

Nonviral gene transfer strategies for the vasculature.

Young JL, Dean DA

Microcirculation : the official journal of the Microcirculatory Society, Inc.. 2002 January 9 (1):35-49. Epub 1900 01 01.

High-level gene transfer to the cornea using electroporation.

Blair-Parks K, Weston BC, Dean DA

The journal of gene medicine.. 2002 4 (1):92-100. Epub 1900 01 01.

The smooth muscle gamma-actin gene is androgen responsive in prostate epithelia.

Filmore RA, Dean DA, Zimmer WE

Gene expression.. 2002 10 (5-6):201-11. Epub 1900 01 01.

Gene therapy: if at first you don't succeed..

Dean DA, Perkin RA

American family physician.. 2001 May 163 (9):1707, 1710, 1715-6. Epub 1900 01 01.

Muscle-specific enhancement of gene expression by incorporation of SV40 enhancer in the expression plasmid.

Li S, MacLaughlin FC, Fewell JG, Gondo M, Wang J, Nicol F, Dean DA, Smith LC

Gene therapy.. 2001 March 8 (6):494-7. Epub 1900 01 01.

Serine/threonine protein phosphatase 5 (PP5) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling.

Dean DA, Urban G, Aragon IV, Swingle M, Miller B, Rusconi S, Bueno M, Dean NM, Honkanen RE

BMC cell biology.. 2001 2 :6. Epub 05/17/2001.

Peptide nucleic acids: versatile tools for gene therapy strategies.

Dean DA

Advanced drug delivery reviews.. 2000 November 1544 (2-3):81-95. Epub 1900 01 01.

Store-operated calcium entry and increased endothelial cell permeability.

Norwood N, Moore TM, Dean DA, Bhattacharjee R, Li M, Stevens T

American journal of physiology. Lung cellular and molecular physiology.. 2000 November 279 (5):L815-24. Epub 1900 01 01.

Dendrimer-assisted patch-clamp sizing of nuclear pores.

Bustamante JO, Michelette ER, Geibel JP, Hanover JA, McDonnell TJ, Dean DA

Pflu?gers Archiv : European journal of physiology.. 2000 April 439 (6):829-37. Epub 1900 01 01.

Calcium, ATP and nuclear pore channel gating.

Bustamante JO, Michelette ER, Geibel JP, Dean DA, Hanover JA, McDonnell TJ

Pflu?gers Archiv : European journal of physiology.. 2000 February 439 (4):433-44. Epub 1900 01 01.

Gene transfer to intact mesenteric arteries by electroporation.

Martin JB, Young JL, Benoit JN, Dean DA

Journal of vascular research.. 2000 37 (5):372-80. Epub 1900 01 01.

Sequence requirements for plasmid nuclear import.

Dean DA, Dean BS, Muller S, Smith LC

Experimental cell research.. 1999 December 15253 (2):713-22. Epub 1900 01 01.

Optimization of folate-conjugated liposomal vectors for folate receptor-mediated gene therapy.

Reddy JA, Dean D, Kennedy MD, Low PS

Journal of pharmaceutical sciences.. 1999 November 88 (11):1112-8. Epub 1900 01 01.

Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences.

Wilson GL, Dean BS, Wang G, Dean DA

The Journal of biological chemistry.. 1999 July 30274 (31):22025-32. Epub 1900 01 01.

Peptide nucleic acid (PNA) binding-mediated induction of human gamma-globin gene expression.

Wang G, Xu X, Pace B, Dean DA, Glazer PM, Chan P, Goodman SR, Shokolenko I

Nucleic acids research.. 1999 July 127 (13):2806-13. Epub 1900 01 01.

Nuclear targeting of plasmid DNA in human corneal cells.

Dean DA, Byrd JN, Dean BS

Current eye research.. 1999 July 19 (1):66-75. Epub 1900 01 01.

Cell-specific nuclear import of plasmid DNA.

Vacik J, Dean BS, Zimmer WE, Dean DA

Gene therapy.. 1999 June 6 (6):1006-14. Epub 1900 01 01.

Membranes of herpes simplex virus type-1-infected human corneal epithelial cells are not permeabilized to macromolecules and therefore do not release IL-1alpha.

Tran MT, Dean DA, Lausch RN, Oakes JE

Virology.. 1998 April 25244 (1):74-8. Epub 1900 01 01.

Import of plasmid DNA into the nucleus is sequence specific.

Dean DA

Experimental cell research.. 1997 February 1230 (2):293-302. Epub 1900 01 01.

Essential role of the Vp2 and Vp3 DNA-binding domain in simian virus 40 morphogenesis.

Dean DA, Li PP, Lee LM, Kasamatsu H

Journal of virology.. 1995 February 69 (2):1115-21. Epub 1900 01 01.

Signal- and energy-dependent nuclear transport of SV40 Vp3 by isolated nuclei. Establishment of a filtration assay for nuclear protein import.

Dean DA, Kasamatsu H

The Journal of biological chemistry.. 1994 February 18269 (7):4910-6. Epub 1900 01 01.

Identification of a DNA binding domain in simian virus 40 capsid proteins Vp2 and Vp3.

Clever J, Dean DA, Kasamatsu H

The Journal of biological chemistry.. 1993 October 5268 (28):20877-83. Epub 1900 01 01.

Interaction between maltose-binding protein and the membrane-associated maltose transporter complex in Escherichia coli.

Dean DA, Hor LI, Shuman HA, Nikaido H

Molecular microbiology.. 1992 August 6 (15):2033-40. Epub 1900 01 01.

Regulation of the maltose transport system of Escherichia coli by the glucose-specific enzyme III of the phosphoenolpyruvate-sugar phosphotransferase system. Characterization of inducer exclusion-resistant mutants and reconstitution of inducer exclusion in proteoliposomes.

Dean DA, Reizer J, Nikaido H, Saier MH

The Journal of biological chemistry.. 1990 December 5265 (34):21005-10. Epub 1900 01 01.

The role of ATP as the energy source for maltose transport in Escherichia coli.

Dean DA, Davidson AL, Nikaido H

Research in microbiology. 1990 141 (3):348-52. Epub 1900 01 01.

Maltose transport in membrane vesicles of Escherichia coli is linked to ATP hydrolysis.

Dean DA, Davidson AL, Nikaido H

Proceedings of the National Academy of Sciences of the United States of America.. 1989 December 86 (23):9134-8. Epub 1900 01 01.

Active transport of maltose in membrane vesicles obtained from Escherichia coli cells producing tethered maltose-binding protein.

Dean DA, Fikes JD, Gehring K, Bassford PJ, Nikaido H

Journal of bacteriology.. 1989 January 171 (1):503-10. Epub 1900 01 01.

Books

Novel Gene Therapy Approaches (2013)

Chapter: Extracellular and Intracellular Barriers to Non-Viral Gene Transfer

Authors: Gottfried, L., and D. A. Dean

Publisher: Intech Press, Rijecka, Croatia 2013

Electroporation Protocols: Experimental and Clinical Medicine (2013)

Chapter: Electroporation-mediated Gene Delivery to the Lungs

Authors: Young, J. L., M. Barravecchia, and D. A. Dean

Publisher: Humana Press, Totowa, NJ 2013

Optical Imaging Techniques: A Laboratory Manua (2010)

Chapter: Nonviral gene delivery

Authors: Dean, D. A., and J. Z. Gasiorowski

Publisher: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 2010

Electroporation in Science and Medicine (2010)

Chapter: Electroporation-mediated gene transfer to the lung

Authors: Dean, DA

Publisher: Springer, New York, NY 2010

Electroporation in Science and Medicine (2010)

Chapter: Electrotransfer of DNA

Authors: Escoffre, J. M., M. P. Rols, and D. A. Dean

Publisher: Springer, New York, NY 2010

Electroporation Protocols: Experimental and Clinical Medicine (in press). (2007)

Chapter: Electroporation-mediated Gene Delivery to the Lungs.

Authors: Zhou, R., J. E. Norton, and D. A. Dean

Publisher: S. Li (Ed). Humana Press, Totowa, NJ 2007

Nucleocytoplasmic Transport (2005)

Chapter: Nuclear import of DNA. .

Authors: Gokay, K. E., and D. A. Dean.

Publisher: T. Tzvi and V. Citovsky (Eds). Landes Bioscience, Georgetown, TX. 2005

Live Cell Imaging: A Laboratory Manual (2004)

Chapter: Improving gene delivery and expression of GFP by cyclic stretch.

Authors: Dean, D. A.

Publisher: D. L. Spector and R. D. Goldman (Eds). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 2004

Pharmaceutical perspectives of nucleic acid-based therapeutics (2002)

Chapter: Nucleocytoplasmic trafficking

Authors: Dean, D. A.

Publisher: R. I. Mahato (Ed). Harwood Academic Publishers, London 2002