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Kathleen E. McGrath, Ph.D.

Kathleen E. McGrath, Ph.D.

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Office (585) 275-5073

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About Me

Faculty Appointments

Research Associate Professor - Department of Pediatrics, Hematology and Oncology (SMD)

Credentials

Education

PhD | University of Rochester. Biology. 1993

MS | University of Rochester. Biology. 1987

BA | Reed College. Biology. 1983

Awards

Leon Wheeless Innovation in Cytometry Award. 2007

Research

Dr. McGrath's research interests are:

1) Emergence of hematopoiesis in murine embryo and ES/iPS cells
2) Recovery of hematopoiesis post radiation
3) Imaging flow cytometry for examination of rare populations
4) Roles of early erythroid-myeloid progenitors during embryogenesis...
Dr. McGrath's research interests are:

1) Emergence of hematopoiesis in murine embryo and ES/iPS cells
2) Recovery of hematopoiesis post radiation
3) Imaging flow cytometry for examination of rare populations
4) Roles of early erythroid-myeloid progenitors during embryogenesis

Dr. McGrath is a Research Assistant Professor working within Jim Palis' research group. Her research interests are in how the hematopoietic system assembled both initially in the embryo and during repair after radiation damage in the adult. She applies that paradigm to the development of the blood system in the mouse embryo where overlapping waves of stem cells emergence, each with different lineage and self-renewal potentials. Similarly, regeneration of hematopoiesis in irradiated adult bone marrow is regulated through compartments of blood cells precursors and progenitors with differential sensitivities and responses to damage. Her work relies on prospectively discriminating these cells with different potentials and understanding the signaling that has determined their fate.

Recently, Dr. McGrath has focused on a unique embryonic hematopoietic progenitor with potential for short term production of erythroid and myeloid cells that can also be identified in embryonic stem cells and can function when transfused in adults. Additionally, her studies of recovery of hematopoietic stem cells as well megakaryocytes after radiation are addressing mitigation of short term thrombocytopenia as well as long term stem cell dysfunction. These studies have benefited from her expertise in flow cytometry including pioneering the use of imaging flow cytometry in analysis of development and repair from the most primitive progenitors to mature blood cells focusing on erythroid and megakaryocyte lineages.

Publications

Journal Articles

BMI1 regulates human erythroid self-renewal through both gene repression and gene activation.

McGrath KE, Koniski AD, Murphy K, Getman M, An HH, Schulz VP, Kim AR, Zhang B, Schofield TL, Papoin J, Blanc L, Kingsley PD, Westhoff CM, Gallagher PG, Chou ST, Steiner LA, Palis J

bioRxiv : the preprint server for biology.. 2024 February 6 Epub 02/06/2024.

Transfusion of Adult, but Not Neonatal, Platelets Promotes Monocyte Trafficking in Neonatal Mice.

Maurya P, Ture SK, Li C, Scheible KM, McGrath KE, Palis J, Morrell CN

Arteriosclerosis, thrombosis, and vascular biology.. 2023 June 43 (6):873-885. Epub 03/23/2023.

Circulating primitive murine erythroblasts undergo complex proteomic and metabolomic changes during terminal maturation.

Nemkov T, Kingsley PD, Dzieciatkowska M, Malik J, McGrath K, Hansen KC, D'Alessandro A, Palis J

Blood advances.. 2022 May 246 (10):3072-3089. Epub 1900 01 01.

Long-acting PGE2 and Lisinopril Mitigate H-ARS.

Saunders J, Niswander LM, McGrath KE, Koniski A, Catherman SC, Ture SK, Medhora M, Kingsley PD, Calvi LM, Williams JP, Morrell CN, Palis J

Radiation research.. 2021 September 1196 (3):284-296. Epub 1900 01 01.

Mds1, an inducible Cre allele specific to adult-repopulating hematopoietic stem cells.

Zhang Y, McGrath KE, Ayoub E, Kingsley PD, Yu H, Fegan K, McGlynn KA, Rudzinskas S, Palis J, Perkins AS

Cell reports.. 2021 August 1736 (7):109562. Epub 1900 01 01.

Lung megakaryocytes are immune modulatory cells.

Pariser DN, Hilt ZT, Ture SK, Blick-Nitko SK, Looney MR, Cleary SJ, Roman-Pagan E, Saunders Ii J, Georas SN, Veazey JM, Madere F, Tesoro Santos L, Arne AM, Huynh NP, Livada AC, Guerrero-Martin SM, Lyons CE, Metcalf Pate KA, McGrath KE, Palis J, Morrell C

The Journal of clinical investigation.. 2021 January 4131 (1)Epub 1900 01 01.

Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B.

Frisch BJ, Hoffman CM, Latchney SE, LaMere MW, Myers J, Ashton J, Li AJ, Saunders J, Palis J, Perkins AS, McCabe A, Smith JN, McGrath KE, Rivera-Escalera F, McDavid A, Liesveld JL, Korshunov VA, Elliott MR, MacNamara KC, Becker MW, Calvi LM

JCI insight.. 2019 April 185 (10)Epub 04/18/2019.

Platelet-derived ?2M regulates monocyte inflammatory responses.

Hilt ZT, Pariser DN, Ture SK, Mohan A, Quijada P, Asante A, Cameron SJ, Sterling JA, Merkel AR, Johanson AL, Jenkins JL, Small EM, McGrath KE, Palis J, Elliott MR, Morrell CN

JCI insight.. 2019 March 74 (5)Epub 03/07/2019.

regulates age-dependent differences in murine platelet function.

Stolla MC, Catherman SC, Kingsley PD, Rowe RG, Koniski AD, Fegan K, Vit L, McGrath KE, Daley GQ, Palis J

Blood advances.. 2019 January 83 (1):72-82. Epub 1900 01 01.

EVI1 overexpression reprograms hematopoiesis via upregulation of Spi1 transcription.

Ayoub E, Wilson MP, McGrath KE, Li AJ, Frisch BJ, Palis J, Calvi LM, Zhang Y, Perkins AS

Nature communications.. 2018 October 129 (1):4239. Epub 10/12/2018.

Kit ligand has a critical role in mouse yolk sac and aorta-gonad-mesonephros hematopoiesis.

Azzoni E, Frontera V, McGrath KE, Harman J, Carrelha J, Nerlov C, Palis J, Jacobsen SEW, de Bruijn MF

EMBO reports.. 2018 October 19 (10)Epub 08/30/2018.

Analysis of Erythropoiesis Using Imaging Flow Cytometry.

Kalfa T, McGrath KE

Methods in molecular biology.. 2018 1698 :175-192. Epub 1900 01 01.

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.

Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J

Scientific reports.. 2017 July 127 (1):5164. Epub 07/12/2017.

Delineating stages of erythropoiesis using imaging flow cytometry.

McGrath KE, Catherman SC, Palis J

Methods : a companion to Methods in enzymology.. 2017 January 1112 :68-74. Epub 08/28/2016.

Analysis of Erythropoiesis using Imaging Flow Cytometry

Kalfa, T.; McGrath, K.E.

Methods in Molecular Biology: Erythropoiesis-Methods and Protocols. 2017; .

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating

Huang, Y.H.; Delgadillo, L.; Cyr, K.; Kingsley, P.; An, X.; McGrath, K.E.; Narla, M.; Conboy, J.; Waugh, R.; Wan,J.; Palis, J.

Scientific Reports. 2017; .

EKLF/KLF1-regulated cell cycle exit is essential for erythroblast enucleation.

Gnanapragasam MN, McGrath KE, Catherman S, Xue L, Palis J, Bieker JJ

Blood.. 2016 September 22128 (12):1631-41. Epub 08/01/2016.

Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity.

Frame JM, Fegan KH, Conway SJ, McGrath KE, Palis J

Stem cells.. 2016 February 34 (2):431-44. Epub 10/23/2015.

Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes.

Niswander LM, Palis J, McGrath KE

Methods in molecular biology.. 2016 1389 :265-77. Epub 1900 01 01.

A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis.

Liang R, Campreciós G, Kou Y, McGrath K, Nowak R, Catherman S, Bigarella CL, Rimmelé P, Zhang X, Gnanapragasam MN, Bieker JJ, Papatsenko D, Ma'ayan A, Bresnick E, Fowler V, Palis J, Ghaffari S

PLoS genetics.. 2015 October 11 (10):e1005526. Epub 10/09/2015.

Utilization of imaging flow cytometry to define intermediates of megakaryopoiesis in vivo and in vitro.

McGrath KE

Journal of immunological methods.. 2015 August 423 :45-51. Epub 03/17/2015.

Distinct Sources of Hematopoietic Progenitors Emerge before HSCs and Provide Functional Blood Cells in the Mammalian Embryo.

McGrath KE, Frame JM, Fegan KH, Bowen JR, Conway SJ, Catherman SC, Kingsley PD, Koniski AD, Palis J

Cell reports.. 2015 June 3011 (12):1892-904. Epub 06/18/2015.

Bmi-1 Regulates Extensive Erythroid Self-Renewal.

Kim AR, Olsen JL, England SJ, Huang YS, Fegan KH, Delgadillo LF, McGrath KE, Kingsley PD, Waugh RE, Palis J

Stem cell reports.. 2015 June 94 (6):995-1003. Epub 05/28/2015.

Emergence of the neutrophil lineage in the mammalian embryo.

McGrath KE, Fegan K, Catherman S, Palis J

Experimental hematology.. 2014 August 42 (8 Suppl):S13. Epub 1900 01 01.

SDF-1 dynamically mediates megakaryocyte niche occupancy and thrombopoiesis at steady state and following radiation injury.

Niswander LM, Fegan KH, Kingsley PD, McGrath KE, Palis J

Blood.. 2014 July 10124 (2):277-86. Epub 04/15/2014.

Red cell island dances: switching hands.

McGrath KE

Blood.. 2014 June 19123 (25):3847-8. Epub 1900 01 01.

Improved quantitative analysis of primary bone marrow megakaryocytes utilizing imaging flow cytometry.

Niswander LM, McGrath KE, Kennedy JC, Palis J

Cytometry. Part A : the journal of the International Society for Analytical Cytology.. 2014 April 85 (4):302-12. Epub 01/16/2014.

The chromatin "landscape" of a murine adult ?-globin gene is unaffected by deletion of either the gene promoter or a downstream enhancer.

Cadiz-Rivera B, Fromm G, de Vries C, Fields J, McGrath KE, Fiering S, Bulger M

PloS one.. 2014 9 (5):e92947. Epub 05/09/2014.

Erythro-myeloid progenitors: "definitive" hematopoiesis in the conceptus prior to the emergence of hematopoietic stem cells.

Frame JM, McGrath KE, Palis J

Blood cells, molecules & diseases.. 2013 December 51 (4):220-5. Epub 10/02/2013.

Ontogeny of erythroid gene expression.

Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J

Blood.. 2013 February 7121 (6):e5-e13. Epub 12/12/2012.

Prostaglandin E2 increases hematopoietic stem cell survival and accelerates hematopoietic recovery after radiation injury.

Porter RL, Georger M, Bromberg O, McGrath KE, Frisch BJ, Becker MW, Calvi LM

Stem cells.. 2013 February 31 (2):372-83. Epub 1900 01 01.

EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress.

Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J

Blood.. 2012 September 20120 (12):2501-11. Epub 08/13/2012.

Primitive erythropoiesis is regulated by miR-126 via nonhematopoietic Vcam-1+ cells.

Sturgeon CM, Chicha L, Ditadi A, Zhou Q, McGrath KE, Palis J, Hammond SM, Wang S, Olson EN, Keller G

Developmental cell.. 2012 July 1723 (1):45-57. Epub 06/28/2012.

An embryonic stage-specific enhancer within the murine ?-globin locus mediates domain-wide histone hyperacetylation.

Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M

Blood.. 2011 May 12117 (19):5207-14. Epub 02/14/2011.

A transient definitive erythroid lineage with unique regulation of the ?-globin locus in the mammalian embryo.

McGrath KE, Frame JM, Fromm GJ, Koniski AD, Kingsley PD, Little J, Bulger M, Palis J

Blood.. 2011 April 28117 (17):4600-8. Epub 03/04/2011.

Sublethal radiation injury uncovers a functional transition during erythroid maturation.

Peslak SA, Wenger J, Bemis JC, Kingsley PD, Frame JM, Koniski AD, Chen Y, Williams JP, McGrath KE, Dertinger SD, Palis J

Experimental hematology.. 2011 April 39 (4):434-45. Epub 02/01/2011.

Immature erythroblasts with extensive ex vivo self-renewal capacity emerge from the early mammalian fetus.

England SJ, McGrath KE, Frame JM, Palis J

Blood.. 2011 March 3117 (9):2708-17. Epub 12/02/2010.

CXCR7 protein is not expressed on human or mouse leukocytes.

Berahovich RD, Zabel BA, Penfold ME, Lewén S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ

The Journal of immunology : official journal of the American Association of Immunologists.. 2010 November 1185 (9):5130-9. Epub 10/01/2010.

Primitive erythropoiesis in the mammalian embryo.

Palis J, Malik J, McGrath KE, Kingsley PD

The International journal of developmental biology.. 2010 54 (6-7):1011-8. Epub 1900 01 01.

Multispectral imaging of hematopoietic cells: where flow meets morphology.

McGrath KE, Bushnell TP, Palis J

Journal of immunological methods.. 2008 July 31336 (2):91-7. Epub 05/15/2008.

All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac.

Lux CT, Yoshimoto M, McGrath K, Conway SJ, Palis J, Yoder MC

Blood.. 2008 April 1111 (7):3435-8. Epub 10/11/2007.

Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb.

Tober J, McGrath KE, Palis J

Blood.. 2008 March 1111 (5):2636-9. Epub 01/03/2008.

Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus.

McGrath KE, Kingsley PD, Koniski AD, Porter RL, Bushnell TP, Palis J

Blood.. 2008 February 15111 (4):2409-17. Epub 11/21/2007.

The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesis.

Tober J, Koniski A, McGrath KE, Vemishetti R, Emerson R, de Mesy-Bentley KK, Waugh R, Palis J

Blood.. 2007 February 15109 (4):1433-41. Epub 10/24/2006.

"Maturational" globin switching in primary primitive erythroid cells.

Kingsley PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, Palis J

Blood.. 2006 February 15107 (4):1665-72. Epub 11/01/2005.

The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesis

Tober,J. M.; Koniski,A.; McGrath,K. E.; Vemishetti,R.; Emerson,R.; de Mesy-Bentley,K. K.; Waugh,R.; Palis,J.;.

Blood. 2006; .

Hematopoiesis in the yolk sac: more than meets the eye.

McGrath KE, Palis J

Experimental hematology.. 2005 September 33 (9):1021-8. Epub 1900 01 01.

Airway injury in lung disease pathophysiology: selective depletion of airway stem and progenitor cell pools potentiates lung inflammation and alveolar dysfunction.

Reynolds SD, Giangreco A, Hong KU, McGrath KE, Ortiz LA, Stripp BR

American journal of physiology. Lung cellular and molecular physiology.. 2004 December 287 (6):L1256-65. Epub 08/06/2004.

Circulation is established in a stepwise pattern in the mammalian embryo.

McGrath KE, Koniski AD, Malik J, Palis J

Blood.. 2003 March 1101 (5):1669-76. Epub 10/24/2002.

Onset of cardiac function during early mouse embryogenesis coincides with entry of primitive erythroblasts into the embryo proper.

Ji RP, Phoon CK, Aristizábal O, McGrath KE, Palis J, Turnbull DH

Circulation research.. 2003 February 792 (2):133-5. Epub 1900 01 01.

Serglycin proteoglycan synthesis in the murine uterine decidua and early embryo.

Keith Ho HC, McGrath KE, Brodbeck KC, Palis J, Schick BP

Biology of reproduction.. 2001 June 64 (6):1667-76. Epub 1900 01 01.

Subtractive hybridization reveals tissue-specific expression of ahnak during embryonic development.

Kingsley PD, McGrath KE, Maltby KM, Koniski AD, Ramchandran R, Palis J

Development, growth & differentiation.. 2001 April 43 (2):133-43. Epub 1900 01 01.

Embryonic expression and function of the chemokine SDF-1 and its receptor, CXCR4.

McGrath KE, Koniski AD, Maltby KM, McGann JK, Palis J

Developmental biology.. 1999 September 15213 (2):442-56. Epub 1900 01 01.

Convergent control of serotonin transporter expression by glucocorticoids and cocaine in fetal and neonatal rat brain.

McGrath KE, Seidler FJ, Slotkin TA

Brain research. Developmental brain research.. 1997 December 19104 (1-2):209-13. Epub 1900 01 01.

Expression of homeobox genes, including an insulin promoting factor, in the murine yolk sac at the time of hematopoietic initiation.

McGrath KE, Palis J

Molecular reproduction and development.. 1997 October 48 (2):145-53. Epub 1900 01 01.

An abundant nucleolar phosphoprotein is associated with ribosomal DNA in Tetrahymena macronuclei.

McGrath KE, Smothers JF, Dadd CA, Madireddi MT, Gorovsky MA, Allis CD

Molecular biology of the cell.. 1997 January 8 (1):97-108. Epub 1900 01 01.

Initiation of hematopoiesis and vasculogenesis in murine yolk sac explants.

Palis J, McGrath KE, Kingsley PD

Blood.. 1995 July 186 (1):156-63. Epub 1900 01 01.

Regulation and evolution of the single alpha-tubulin gene of the ciliate Tetrahymena thermophila.

McGrath KE, Yu SM, Heruth DP, Kelly AA, Gorovsky MA

Cell motility and the cytoskeleton.. 1994 27 (3):272-83. Epub 1900 01 01.

Perspectives on tubulin isotype function and evolution based on the observation that Tetrahymena thermophila microtubules contain a single alpha- and beta-tubulin.

Gaertig J, Thatcher TH, McGrath KE, Callahan RC, Gorovsky MA

Cell motility and the cytoskeleton.. 1993 25 (3):243-53. Epub 1900 01 01.

Books

Polyamines: Basic Clinical Aspects (1984)

Chapter: Polyamine Stress: A Variable Nonspecific Component of Disease?

Authors: Campbell RA, McGrath KE

Publisher: VNU Science Press, The Netherlands 1984