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Harold C. Smith, Ph.D.

Harold C. Smith, Ph.D.

About Me

Faculty Appointments

Professor Emeritus - Department of Biochemistry and Biophysics (SMD)

Credentials

Education

PhD | St Univ at Buffalo. Molecular Biology. 1982

MA | St Univ at Buffalo. Molecular Biology. 1980

MS | Purdue University. Veterinary Medicine. 1978

BS | Purdue University. Biology. 1975

Research

Normal and cancer cells express more diversity in proteins than can be accounted for by the predicted number of expressed genomic DNA sequence. Expansion of the genomically encoded expressed sequences through alternative processing of RNA, such as mRNA editing, is a logical hypothesis for how protei...
Normal and cancer cells express more diversity in proteins than can be accounted for by the predicted number of expressed genomic DNA sequence. Expansion of the genomically encoded expressed sequences through alternative processing of RNA, such as mRNA editing, is a logical hypothesis for how protein diversity and variations seen as tissue-specific and regulated expression of proteins can be achieved. The specific focus of the research is to identify and characterize novel mammalian mRNA editing systems that employ a zinc-dependent deamination mechanism for the post-transcriptional conversion of cytidine to uridine at select sites within mRNAs. Computational modeling has suggested a family of mammalian enzymes known as Cytidine Deaminases Active on RNA or CDARs as responsible for C to U editing of mRNAs.

The expression of these enzymes in biology suggests that mRNA editing may be involved in numerous physiological processes and could be manipulated for the prevention of cardiovascular disease, HIV infection and cancer, and is also necessary for production of antibodies in B lymphocytes. Recent evidence indicates that the enzyme involved in suppressing HIV-1 infectivity (CEM15) and the enzyme that promotes antibody production (AID) may act to mutate deoxy cytidine in DNA rather than or in addition to RNA.

Our research involves molecular biology and protein techniques, DNA microarray analyses and computational biology to identify the mRNAs that are edited by CDARs and to determine the biological consequence of these editing events in terms of the predicted changes in the types of protein structures and functions that can be expressed. Our studies have demonstrated how cytidine to uridine mRNA editing contributes to expansion in the diversity of expressed mRNA sequences known collectively as the transcriptome.

We are also evaluating the regulatory mechanisms controlling the expression of editing factors and their localization in the cell nucleus. The development of this new information will establish an important new annotation of the human genome that will serve as a frame of reference for studies of proteins involved in health and disease and mechanisms regulating their expression.

Publications

Journal Articles

A Novel Ebola Virus VP40 Matrix Protein-Based Screening for Identification of Novel Candidate Medical Countermeasures.

Bennett RP, Finch CL, Postnikova EN, Stewart RA, Cai Y, Yu S, Liang J, Dyall J, Salter JD, Smith HC, Kuhn JH

Viruses.. 2020 December 3113 (1)Epub 12/31/2020.

Modeling the Embrace of a Mutator: APOBEC Selection of Nucleic Acid Ligands.

Salter JD, Smith HC

Trends in biochemical sciences.. 2018 August 43 (8):606-622. Epub 05/23/2018.

A New Class of Antiretroviral Enabling Innate Immunity by Protecting APOBEC3 from HIV Vif-Dependent Degradation.

Bennett RP, Salter JD, Smith HC

Trends in molecular medicine.. 2018 May 24 (5):507-520. Epub 03/30/2018.

RNA binding to APOBEC deaminases; Not simply a substrate for C to U editing.

Smith HC

RNA biology.. 2017 September 214 (9):1153-1165. Epub 11/21/2016.

HIV-1 Frameshift RNA-Targeted Triazoles Inhibit Propagation of Replication-Competent and Multi-Drug-Resistant HIV in Human Cells.

Hilimire TA, Chamberlain JM, Anokhina V, Bennett RP, Swart O, Myers JR, Ashton JM, Stewart RA, Featherston AL, Gates K, Helms ED, Smith HC, Dewhurst S, Miller BL

ACS chemical biology.. 2017 June 1612 (6):1674-1682. Epub 05/05/2017.

DNA mutagenic activity and capacity for HIV-1 restriction of the cytidine deaminase APOBEC3G depend on whether DNA or RNA binds to tyrosine 315.

Polevoda B, Joseph R, Friedman AE, Bennett RP, Greiner R, De Zoysa T, Stewart RA, Smith HC

The Journal of biological chemistry.. 2017 May 26292 (21):8642-8656. Epub 04/05/2017.

An analog of camptothecin inactive against Topoisomerase I is broadly neutralizing of HIV-1 through inhibition of Vif-dependent APOBEC3G degradation.

Bennett RP, Stewart RA, Hogan PA, Ptak RG, Mankowski MK, Hartman TL, Buckheit RW, Snyder BA, Salter JD, Morales GA, Smith HC

Antiviral research.. 2016 December 136 :51-59. Epub 11/05/2016.

Distal Radius Fracture Outcomes and Rehabilitation.

Ikpeze TC, Smith HC, Lee DJ, Elfar JC

Geriatric orthopaedic surgery & rehabilitation.. 2016 December 7 (4):202-205. Epub 09/22/2016.

Structural and functional assessment of APOBEC3G macromolecular complexes.

Polevoda B, McDougall WM, Bennett RP, Salter JD, Smith HC

Methods : a companion to Methods in enzymology.. 2016 September 1107 :10-22. Epub 03/14/2016.

The APOBEC Protein Family: United by Structure, Divergent in Function.

Salter JD, Bennett RP, Smith HC

Trends in biochemical sciences.. 2016 July 41 (7):578-594. Epub 06/06/2016.

N-Methylation as a Strategy for Enhancing the Affinity and Selectivity of RNA-binding Peptides: Application to the HIV-1 Frameshift-Stimulating RNA.

Hilimire TA, Bennett RP, Stewart RA, Garcia-Miranda P, Blume A, Becker J, Sherer N, Helms ED, Butcher SE, Smith HC, Miller BL

ACS chemical biology.. 2016 January 1511 (1):88-94. Epub 11/03/2015.

RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates.

Polevoda B, McDougall WM, Tun BN, Cheung M, Salter JD, Friedman AE, Smith HC

Nucleic acids research.. 2015 October 3043 (19):9434-45. Epub 09/30/2015.

Structural insights for HIV-1 therapeutic strategies targeting Vif.

Salter JD, Morales GA, Smith HC

Trends in biochemical sciences.. 2014 September 39 (9):373-80. Epub 08/12/2014.

High-affinity recognition of HIV-1 frameshift-stimulating RNA alters frameshifting in vitro and interferes with HIV-1 infectivity.

Ofori LO, Hilimire TA, Bennett RP, Brown NW, Smith HC, Miller BL

Journal of medicinal chemistry.. 2014 February 1357 (3):723-32. Epub 01/15/2014.

The multifaceted roles of RNA binding in APOBEC cytidine deaminase functions.

Prohaska KM, Bennett RP, Salter JD, Smith HC

Wiley interdisciplinary reviews. RNA.. 2014 5 (4):493-508. Epub 03/24/2014.

Functions and regulation of the APOBEC family of proteins.

Smith HC, Bennett RP, Kizilyer A, McDougall WM, Prohaska KM

Seminars in cell & developmental biology.. 2012 May 23 (3):258-68. Epub 10/06/2011.

Direct evidence that RNA inhibits APOBEC3G ssDNA cytidine deaminase activity.

McDougall WM, Smith HC

Biochemical and biophysical research communications.. 2011 September 9412 (4):612-7. Epub 08/11/2011.

Deaminase activity on single-stranded DNA (ssDNA) occurs in vitro when APOBEC3G cytidine deaminase forms homotetramers and higher-order complexes.

McDougall WM, Okany C, Smith HC

The Journal of biological chemistry.. 2011 September 2286 (35):30655-30661. Epub 07/07/2011.

APOBEC3G: a double agent in defense.

Smith HC

Trends in biochemical sciences.. 2011 May 36 (5):239-44. Epub 01/14/2011.

Metabolic regulation of APOBEC-1 complementation factor trafficking in mouse models of obesity and its positive correlation with the expression of ApoB protein in hepatocytes.

Galloway CA, Ashton J, Sparks JD, Mooney RA, Smith HC

Biochimica et biophysica acta.. 2010 November 1802 (11):976-85. Epub 06/09/2010.

APOBEC-1 complementation factor (ACF) forms RNA-dependent multimers.

Galloway CA, Kumar A, Krucinska J, Smith HC

Biochemical and biophysical research communications.. 2010 July 16398 (1):38-43. Epub 06/10/2010.

The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells.

Galloway CA, Smith HC

Biochemical and biophysical research communications.. 2010 January 1391 (1):659-63. Epub 11/20/2009.

A hydrodynamic analysis of APOBEC3G reveals a monomer-dimer-tetramer self-association that has implications for anti-HIV function.

Salter JD, Krucinska J, Raina J, Smith HC, Wedekind JE

Biochemistry.. 2009 November 1748 (45):10685-7. Epub 1900 01 01.

APOBEC3G subunits self-associate via the C-terminal deaminase domain.

Bennett RP, Salter JD, Liu X, Wedekind JE, Smith HC

The Journal of biological chemistry.. 2008 November 28283 (48):33329-36. Epub 10/08/2008.

Nuclear Exclusion of the HIV-1 host defense factor APOBEC3G requires a novel cytoplasmic retention signal and is not dependent on RNA binding.

Bennett RP, Presnyak V, Wedekind JE, Smith HC

The Journal of biological chemistry.. 2008 March 21283 (12):7320-7. Epub 12/28/2007.

RNA and DNA Editing: Molecular Mechanisms and Their Integration into Biological Systems

Smith, H. C.;.

(H.C. Smith, ed) 16 chapters, Wiley Press, NY, 2008. 2008; .

The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G.

Miller JH, Presnyak V, Smith HC

Retrovirology.. 2007 November 244 :81. Epub 11/24/2007.

Functional characterization of APOBEC-1 complementation factor phosphorylation sites.

Lehmann DM, Galloway CA, MacElrevey C, Sowden MP, Wedekind JE, Smith HC

Biochimica et biophysica acta.. 2007 March 1773 (3):408-18. Epub 12/08/2006.

APOBEC3G levels predict rates of progression to AIDS

Jin, X.; Wu, H.; Smith, H.C.;.

Retrovirology. 2007; 4(3): 20-7.

The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G

Miller, H. J.; Presnyak, V.; Smith, H.C.;.

Retrovirology. 2007; 4(3): 81-91.

Measuring editing activity and identifying cytidine-to-uridine mRNA editing factors in cells and biochemical isolates.

Smith HC

Methods in enzymology.. 2007 424 :389-416. Epub 1900 01 01.

Nanostructures of APOBEC3G support a hierarchical assembly model of high molecular mass ribonucleoprotein particles from dimeric subunits.

Wedekind JE, Gillilan R, Janda A, Krucinska J, Salter JD, Bennett RP, Raina J, Smith HC

The Journal of biological chemistry.. 2006 December 15281 (50):38122-6. Epub 10/31/2006.

APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic.

Bennett RP, Diner E, Sowden MP, Lees JA, Wedekind JE, Smith HC

Biochemical and biophysical research communications.. 2006 November 10350 (1):214-9. Epub 09/18/2006.

Structural phylogenetic analysis of activation-induced deaminase function.

Ichikawa HT, Sowden MP, Torelli AT, Bachl J, Huang P, Dance GS, Marr SH, Robert J, Wedekind JE, Smith HC, Bottaro A

The Journal of immunology : official journal of the American Association of Immunologists.. 2006 July 1177 (1):355-61. Epub 1900 01 01.

Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factor.

Lehmann DM, Galloway CA, Sowden MP, Smith HC

Nucleic acids research.. 2006 34 (11):3299-308. Epub 07/04/2006.

APOBEC3G/CEM15 (hA3G) mRNA levels associate inversely with human immunodeficiency virus viremia.

Jin X, Brooks A, Chen H, Bennett R, Reichman R, Smith H

Journal of virology.. 2005 September 79 (17):11513-6. Epub 1900 01 01.

Activation induced deaminase: the importance of being specific.

Smith HC, Bottaro A, Sowden MP, Wedekind JE

Trends in genetics : TIG.. 2004 June 20 (6):224-7. Epub 1900 01 01.

The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1.

Xie K, Sowden MP, Dance GS, Torelli AT, Smith HC, Wedekind JE

Proceedings of the National Academy of Sciences of the United States of America.. 2004 May 25101 (21):8114-9. Epub 05/17/2004.

Identification of novel alternative splice variants of APOBEC-1 complementation factor with different capacities to support apolipoprotein B mRNA editing.

Sowden MP, Lehmann DM, Lin X, Smith CO, Smith HC

The Journal of biological chemistry.. 2004 January 2279 (1):197-206. Epub 10/21/2003.

Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business.

Wedekind JE, Dance GS, Sowden MP, Smith HC

Trends in genetics : TIG.. 2003 April 19 (4):207-16. Epub 1900 01 01.

Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase.

Galloway CA, Sowden MP, Smith HC

BioTechniques.. 2003 March 34 (3):524-6, 528, 530. Epub 1900 01 01.

A course director's perspectives on problem-based learning curricula in biochemistry.

Smith HC

Academic medicine : journal of the Association of American Medical Colleges.. 2002 December 77 (12 Pt 1):1189-98. Epub 1900 01 01.

Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.

Dance GS, Sowden MP, Cartegni L, Cooper E, Krainer AR, Smith HC

The Journal of biological chemistry.. 2002 April 12277 (15):12703-9. Epub 01/28/2002.

The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors.

Sowden MP, Ballatori N, Jensen KL, Reed LH, Smith HC

Journal of cell science.. 2002 March 1115 (Pt 5):1027-39. Epub 1900 01 01.

Apolipoprotein B mRNA editing and the reduction in synthesis and secretion of the atherogenic risk factor, apolipoprotein B100 can be effectively targeted through TAT-mediated protein transduction.

Yang Y, Ballatori N, Smith HC

Molecular pharmacology.. 2002 February 61 (2):269-76. Epub 1900 01 01.

Ethanol stimulates apolipoprotein B mRNA editing in the absence of de novo RNA or protein synthesis.

Giangreco A, Sowden MP, Mikityansky I, Smith HC

Biochemical and biophysical research communications.. 2001 December 21289 (5):1162-7. Epub 1900 01 01.

Commitment of apolipoprotein B RNA to the splicing pathway regulates cytidine-to-uridine editing-site utilization.

Sowden MP, Smith HC

The Biochemical journal.. 2001 November 1359 (Pt 3):697-705. Epub 1900 01 01.

Intracellular trafficking determinants in APOBEC-1, the catalytic subunit for cytidine to uridine editing of apolipoprotein B mRNA.

Yang Y, Sowden MP, Yang Y, Smith HC

Experimental cell research.. 2001 July 15267 (2):153-64. Epub 1900 01 01.

Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase.

Dance GS, Beemiller P, Yang Y, Mater DV, Mian IS, Smith HC

Nucleic acids research.. 2001 April 1529 (8):1772-80. Epub 1900 01 01.

Induction of cytidine to uridine editing on cytoplasmic apolipoprotein B mRNA by overexpressing APOBEC-1.

Yang Y, Sowden MP, Smith HC

The Journal of biological chemistry.. 2000 July 28275 (30):22663-9. Epub 1900 01 01.

APOBEC-1 dependent cytidine to uridine editing of apolipoprotein B RNA in yeast.

Dance GS, Sowden MP, Yang Y, Smith HC

Nucleic acids research.. 2000 January 1528 (2):424-9. Epub 1900 01 01.

Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity.

Siddiqui JF, Van Mater D, Sowden MP, Smith HC

Experimental cell research.. 1999 October 10252 (1):154-64. Epub 1900 01 01.

Tissue-specific differences in the role of RNA 3' of the apolipoprotein B mRNA mooring sequence in editosome assembly.

Steinburg MF, Schock D, Backus JW, Smith HC

Biochemical and biophysical research communications.. 1999 September 16263 (1):81-6. Epub 1900 01 01.

Apolipoprotein B mRNA and lipoprotein secretion are increased in McArdle RH-7777 cells by expression of betaine-homocysteine S-methyltransferase.

Sowden MP, Collins HL, Smith HC, Garrow TA, Sparks JD, Sparks CE

The Biochemical journal.. 1999 August 1341 ( Pt 3) (Pt 3):639-45. Epub 1900 01 01.

Ethanol increases apolipoprotein B mRNA editing in rat primary hepatocytes and McArdle cells.

Van Mater D, Sowden MP, Cianci J, Sparks JD, Sparks CE, Ballatori N, Smith HC

Biochemical and biophysical research communications.. 1998 November 18252 (2):334-9. Epub 1900 01 01.

Analysis of protein complexes assembled on apolipoprotein B mRNA for mooring sequence-dependent RNA editing.

Smith HC

Methods : a companion to Methods in enzymology.. 1998 May 15 (1):27-39. Epub 1900 01 01.

Apolipoprotein B RNA sequence 3' of the mooring sequence and cellular sources of auxiliary factors determine the location and extent of promiscuous editing.

Sowden MP, Eagleton MJ, Smith HC

Nucleic acids research.. 1998 April 126 (7):1644-52. Epub 1900 01 01.

A quantitative assay for assessing allelic proportions by iterative gap ligation.

Stewart J, Kozlowski P, Sowden M, Messing E, Smith HC

Nucleic acids research.. 1998 February 1526 (4):961-6. Epub 1900 01 01.

Multiple protein domains determine the cell type-specific nuclear distribution of the catalytic subunit required for apolipoprotein B mRNA editing.

Yang Y, Yang Y, Smith HC

Proceedings of the National Academy of Sciences of the United States of America.. 1997 November 2594 (24):13075-80. Epub 1900 01 01.

Partial characterization of the auxiliary factors involved in apolipoprotein B mRNA editing through APOBEC-1 affinity chromatography.

Yang Y, Yang Y, Kovalski K, Smith HC

The Journal of biological chemistry.. 1997 October 31272 (44):27700-6. Epub 1900 01 01.

A guide to RNA editing.

Smith HC, Gott JM, Hanson MR

RNA.. 1997 October 3 (10):1105-23. Epub 1900 01 01.

Effects of fatty acids on apolipoprotein B secretion by McArdle RH-7777 rat hepatoma cells.

Sparks JD, Collins HL, Sabio I, Sowden MP, Smith HC, Cianci J, Sparks CE

Biochimica et biophysica acta.. 1997 July 121347 (1):51-61. Epub 1900 01 01.

Base-modification mRNA editing through deamination--the good, the bad and the unregulated.

Smith HC, Sowden MP

Trends in genetics : TIG.. 1996 October 12 (10):418-24. Epub 1900 01 01.

Regulation of hepatic apolipoprotein B RNA editing in the genetically obese Zucker rat.

Phung TL, Sowden MP, Sparks JD, Sparks CE, Smith HC

Metabolism: clinical and experimental.. 1996 September 45 (9):1056-8. Epub 1900 01 01.

Determinants involved in regulating the proportion of edited apolipoprotein B RNAs.

Sowden M, Hamm JK, Spinelli S, Smith HC

RNA.. 1996 March 2 (3):274-88. Epub 1900 01 01.

Overexpression of APOBEC-1 results in mooring sequence-dependent promiscuous RNA editing.

Sowden M, Hamm JK, Smith HC

The Journal of biological chemistry.. 1996 February 9271 (6):3011-7. Epub 1900 01 01.

An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing.

Schock D, Kuo SR, Steinburg MF, Bolognino M, Sparks JD, Sparks CE, Smith HC

Proceedings of the National Academy of Sciences of the United States of America.. 1996 February 693 (3):1097-102. Epub 1900 01 01.

The neurofibromatosis type I messenger RNA undergoes base-modification RNA editing.

Skuse GR, Cappione AJ, Sowden M, Metheny LJ, Smith HC

Nucleic acids research.. 1996 February 124 (3):478-85. Epub 1900 01 01.

In vitro reconstitution of apolipoprotein B RNA editing activity from recombinant APOBEC-1 and McArdle cell extracts.

Yang Y, Smith HC

Biochemical and biophysical research communications.. 1996 January 26218 (3):797-801. Epub 1900 01 01.

Proposed nomenclature for the catalytic subunit of the mammalian apolipoprotein B mRNA editing enzyme: APOBEC-1.

Davidson NO, Innerarity TL, Scott J, Smith H, Driscoll DM, Teng B, Chan L

RNA.. 1995 March 1 (1):3. Epub 1900 01 01.

Herpes simplex myelitis as a cause of the acute necrotizing myelitis syndrome

FOLPE, A.; LAPHAM, L.W.; SMITH, H.C.;.

Neurology. 1995; 44(3): 1955-1957.

Herpes simplex myelitis as a cause of acute necrotizing myelitis syndrome.

Folpe A, Lapham LW, Smith HC

Neurology.. 1994 October 44 (10):1955-7. Epub 1900 01 01.

Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.

Backus JW, Schock D, Smith HC

Biochimica et biophysica acta.. 1994 September 131219 (1):1-14. Epub 1900 01 01.

Specific 3' sequences flanking a minimal apolipoprotein B (apoB) mRNA editing 'cassette' are critical for efficient editing in vitro.

Backus JW, Smith HC

Biochimica et biophysica acta.. 1994 January 181217 (1):65-73. Epub 1900 01 01.

Apolipoprotein B mRNA editing in vitro is a zinc-dependent process.

Barnes C, Smith HC

Biochemical and biophysical research communications.. 1993 December 30197 (3):1410-4. Epub 1900 01 01.

Apolipoprotein B mRNA editing: the sequence to the event.

Smith HC

Seminars in cell biology. 1993 August 4 (4):267-78. Epub 1900 01 01.

Extract-specific heterogeneity in high-order complexes containing apolipoprotein B mRNA editing activity and RNA-binding proteins.

Harris SG, Sabio I, Mayer E, Steinberg MF, Backus JW, Sparks JD, Sparks CE, Smith HC

The Journal of biological chemistry.. 1993 April 5268 (10):7382-92. Epub 1900 01 01.

Hypoxic stress induces reversible hypophosphorylation of pRB and reduction in cyclin A abundance independent of cell cycle progression.

Ludlow JW, Howell RL, Smith HC

Oncogene.. 1993 February 8 (2):331-9. Epub 1900 01 01.

Three distinct RNA sequence elements are required for efficient apolipoprotein B (apoB) RNA editing in vitro.

Backus JW, Smith HC

Nucleic acids research.. 1992 November 2520 (22):6007-14. Epub 1900 01 01.

Impaired hepatic apolipoprotein B and E translation in streptozotocin diabetic rats.

Sparks JD, Zolfaghari R, Sparks CE, Smith HC, Fisher EA

The Journal of clinical investigation.. 1992 May 89 (5):1418-30. Epub 1900 01 01.

In vitro apolipoprotein B mRNA editing activity can be modulated by fasting and refeeding rats with a high carbohydrate diet.

Harris SG, Smith HC

Biochemical and biophysical research communications.. 1992 March 16183 (2):899-903. Epub 1900 01 01.

Organization of RNA splicing in the cell nucleus.

Smith HC

Current topics in cellular regulation. 1992 33 :145-66. Epub 1900 01 01.

Apolipoprotein B mRNA sequences 3' of the editing site are necessary and sufficient for editing and editosome assembly.

Backus JW, Smith HC

Nucleic acids research.. 1991 December 2519 (24):6781-6. Epub 1900 01 01.

Induction of the proliferative phenotype in differentiated myogenic cells by hypoxia.

Butler AJ, Eagleton MJ, Wang D, Howell RL, Strauch AR, Khasgiwala V, Smith HC

The Journal of biological chemistry.. 1991 September 25266 (27):18250-8. Epub 1900 01 01.

Oxygen regulated 80 kDa protein and glucose regulated 78kDa protein are identical.

Roll DE, Murphy BJ, Laderoute KR, Sutherland RM, Smith HC

Molecular and cellular biochemistry.. 1991 May 15103 (2):141-8. Epub 1900 01 01.

In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex.

Smith HC, Kuo SR, Backus JW, Harris SG, Sparks CE, Sparks JD

Proceedings of the National Academy of Sciences of the United States of America.. 1991 February 1588 (4):1489-93. Epub 1900 01 01.

Analysis of U1snRNP-specific A protein cross-linked complexes.

Boix J, Habets WJ, van Venrooij WJ, Smith HC

FEBS letters.. 1990 October 15272 (1-2):213-6. Epub 1900 01 01.

Quantitation of endogenous liver apolipoprotein B mRNA editing.

Backus JW, Eagleton MJ, Harris SG, Sparks CE, Sparks JD, Smith HC

Biochemical and biophysical research communications.. 1990 July 31170 (2):513-8. Epub 1900 01 01.

Evidence that a nuclear matrix protein participates in premessenger RNA splicing.

Smith HC, Harris SG, Zillmann M, Berget SM

Experimental cell research.. 1989 June 182 (2):521-33. Epub 1900 01 01.

Immunological evidence for the role of phosphoprotein p68/pI = 7.3 in premessenger RNA splicing.

Liew CC, Smith HC

FEBS letters.. 1989 May 8248 (1-2):101-4. Epub 1900 01 01.

Reversible chemical cross-linking and ribonuclease digestion analysis of the organization of proteins in ribonucleoprotein particles.

Harris SG, Martin TE, Smith HC

Molecular and cellular biochemistry.. 1988 November 84 (1):17-28. Epub 1900 01 01.

Chemical cross-linking of Sm and RNP antigenic proteins.

Harris SG, Hoch SO, Smith HC

Biochemistry.. 1988 June 2827 (13):4595-600. Epub 1900 01 01.

SnRNP core protein enrichment in the nuclear matrix.

Harris SG, Smith HC

Biochemical and biophysical research communications.. 1988 May 16152 (3):1383-7. Epub 1900 01 01.

Ribosomal DNA sequences attached to the nuclear matrix.

Smith HC, Rothblum LI

Biochemical genetics.. 1987 December 25 (11-12):863-79. Epub 1900 01 01.

Ultrastructural and biochemical comparisons of nuclear matrices prepared by high salt or LIS extraction.

Smith HC, Ochs RL, Lin D, Chinault AC

Molecular and cellular biochemistry.. 1987 September 77 (1):49-61. Epub 1900 01 01.

Macromolecular domains containing nuclear protein p107 and U-snRNP protein p28: further evidence for an in situ nuclear matrix.

Smith HC, Ochs RL, Fernandez EA, Spector DL

Molecular and cellular biochemistry.. 1986 May 70 (2):151-68. Epub 1900 01 01.

Redistribution of U-snRNPs during mitosis.

Spector DL, Smith HC

Experimental cell research.. 1986 March 163 (1):87-94. Epub 1900 01 01.

Alterations in chromatin Condensation sate are accompanied by reorganization of nonchromatin domains that contain U-snRNP proteins and nuclear protein p107

SMITH, H.C.; SPECTOR, D.L.; WOODCOCK, C.L.F.; OCHS, R.L.; BHORJEE, J.;.

J. Cell Biol. 1986; 101(3): 560-567.

Distribution of initial and persistent 2-acetylaminofluorene-induced DNA adducts within DNA loops.

Gupta RC, Dighe NR, Randerath K, Smith HC

Proceedings of the National Academy of Sciences of the United States of America.. 1985 October 82 (19):6605-8. Epub 1900 01 01.

The nuclear matrix continues DNA synthesis at in vivo replicational forks.

Tubo RA, Smith HC, Berezney R

Biochimica et biophysica acta.. 1985 July 24825 (3):326-34. Epub 1900 01 01.

Properties of adenoviral DNA bound to the nuclear matrix.

Smith HC, Berezney R, Brewster JM, Rekosh D

Biochemistry.. 1985 February 2624 (5):1197-202. Epub 1900 01 01.

Spatial distribution of DNA loop attachment and replicational sites in the nuclear matrix.

Smith HC, Puvion E, Buchholtz LA, Berezney R

The Journal of cell biology.. 1984 November 99 (5):1794-802. Epub 1900 01 01.

Dynamic domains of DNA polymerase alpha in regenerating rat liver.

Smith HC, Berezney R

Biochemistry.. 1983 June 2122 (13):3042-6. Epub 1900 01 01.

Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system.

Smith HC, Berezney R

Biochemistry.. 1982 December 2121 (26):6751-61. Epub 1900 01 01.

DNA polymerase alpha is tightly bound to the nuclear matrix of actively replicating liver.

Smith HC, Berezney R

Biochemical and biophysical research communications.. 1980 December 3197 (4):1541-7. Epub 1900 01 01.

Books

RNA and DNA Editing: Mechanisms and Their Impact on Biological Systems (2008)

methods in Enzymology (2007)

Chapter: Determination of C to U Editing Activity in Cells and Biochemical Isolates

The Implicit Genome (2006)

Chapter: Editing Informational Content of Expressed DNA Sequences and Their Transcripts

Topics in Current Genetics (2005)

Chapter: Structure and Function of Mammalian Cytidine Deaminases that Mediate Expressed Sequence Diversification

Encyclopedia of Life Sciences (2000)

Chapter: RNA Editing

Methods in Enzymology, RNA Editing (1998)

Chapter: Macromolecular complexes involved in apolipoprotein B mRNA editing

Cell Biology of Trauma (1994)

Chapter: Alterations in cell cycle control factors and the induction of oxygen-regulated stress proteins by hypoxic stress

Current Topics in Cell Regulation (1992)

Chapter: Organization of nuclear mRNA splicing domains

Structural Aspects of Metabolic. Regulation (1989)

Chapter: Ribonucleoprotein organization: from cell nucleus to RNP

The Nuclear Envelope and Nuclear Matrix (1982)

Chapter: Nuclear organization and DNA replication.