Lab Protocols
- General
- Protein Purification and Biochemistry
- Nucleic Acid Protocols
- Thermodynamic Protocols
- Structural Biology Protocols
- Molecular Biology Protocols
- Tissue Culture and Related Protocols
General
Nanodrop UV/VIS Spectrophotometer
Washing Glassware - Dishwasher
Protein Purification and Biochemistry Protocols
Remote Connection to Akta Pures
E. coli Large Scale Induction of Protein Expression
Lysis of E. coli by Sonication
Lysis of E. coli by Avestin press
Protein Purification Stock Buffers
Bacterial Cell Resuspension Buffers
Protein Purification Running Buffers
Purify a Protein with a GST Tag
Purify a Protein with a Histidine Tag
Purify a Protein with Acidic pI
Purify a Protein with Basic pI
Purify a Protein with Thrombin Cleavage Site
Selenomethionine Protein Purification
A280 Protein Concentration Determination
Laemmli Method for SDS-PAGE Gels and Buffers
Prepare Sigma glutathione agarose
PreScission Protease Purification and Use
TEV Protease Purification and Use
Nucleic Acid Protocols
Electrophoretic Mobility Shift Assays
Adjusting PhosphoImager Image Quality
SYBR Gold Staining of Nucleic Acids
Thermodynamic Protocols
BIAcore T100 for kinetic or affinity measurements
Isothermal Titration Calorimetry using Kielkopf VP-ITC
Isothermal Titration Calorimetry using Wedekind PEAQ-ITC
Circular Dichroism Protocol: Jasco in SBBF
Circular Dichroism Protocol: Prior Aviv
Fluoromax-3 Polarizer Alignment
Fluorescence Anisotropy RNA Binding Assay
Equations for Fitting Fluorescence Titration Assay
Electrophoretic Mobility Shift Assay
Amino Acid Analysis to Confirm Concentration for ITC
Effect of Interdomain Linker on Kd
Structural Biology Protocols
Vitrobot for cryoEM Grid Preparation
Dynamic Light Scattering (DLS)
Small Angle X-ray Data Collection at ALS (with Greg Hura's help)
Scaling Pr functions calculated from SAXS data
Remote Data Collection at SSRL
Molecular Biology Protocols
Making LB for Plates and Liquid Media
PCR Primer Design for Subcloning
Restriction Enzyme Digest of DNA Insert and Vector
Gel Purifying Your PCR Product
Analyzing Plasmid DNA by Gel Electrophoresis
Overnight Cultures of Successful Transformants
Freezer stocks of transformed bacteria
Tissue Culture and Related Protocols
Quantitative Real-Time Reverse Transcription-PCR