Recent Publications

• 1. Maji D
  2. Jenkins JL
  3. Boutz PL
  4. Kielkopf CL
  Recurrent Neurodevelopmentally Associated Variants of the Pre-mRNA Splicing Factor U2AF2 Alter RNA Binding Affinities and Interactions.; Biochemistry. 2024 Oct 10.
• 1. Galardi JW
  2. Bela VN
  3. Jeffery N
  4. He X
  5. Glasser E
  6. Loerch S
  7. Jenkins JL
  8. Pulvino MJ
  9. Boutz PL
  10. Kielkopf CL
  A UHM - ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing.; The Journal of biological chemistry. 2022 Jun 30.
• 1. Glasser E
  2. Maji D
  3. Biancon G
  4. Puthenpeedikakkal AMK
  5. Cavender CE
  6. Tebaldi T
  7. Jenkins JL
  8. Mathews DH
  9. Halene S
  10. Kielkopf CL
  Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal.; Nucleic acids research. 2022 May 07.
• 1. Chatrikhi R
  2. Feeney CF
  3. Pulvino MJ
  4. Alachouzos G
  5. MacRae AJ
  6. Falls Z
  7. Rai S
  8. Brennessel WW
  9. Jenkins JL
  10. Walter MJ
  11. Graubert TA
  12. Samudrala R
  13. Jurica MS
  14. Frontier AJ
  15. Kielkopf CL
  A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex.; Cell chemical biology. 2021 Feb 26.
• 1. Maji D
  2. Glasser E
  3. Henderson S
  4. Galardi J
  5. Pulvino MJ
  6. Jenkins JL
  7. Kielkopf CL
  Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.; The Journal of biological chemistry; Vol 295(50). 2020 Dec 11.
• 1. Maji D
  2. Glasser E
  3. Henderson S
  4. Galardi J
  5. Pulvino MJ
  6. Jenkins JL
  7. Kielkopf CL
  Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.; The Journal of biological chemistry. 2020 Oct 05.
• 1. Warnasooriya C
  2. Feeney CF
  3. Laird KM
  4. Ermolenko DN
  5. Kielkopf CL
  A splice site-sensing conformational switch in U2AF2 is modulated by U2AF1 and its recurrent myelodysplasia-associated mutation.; Nucleic acids research; Vol 48(10). 2020 Jun 04.
• 1. Maji D
  2. Grossfield AM
  3. Kielkopf CL
  Structures of SF3b1 reveal a dynamic Achilles heel of spliceosome assembly: Implications for cancer-associated abnormalities and drug discovery.; Biochimica et biophysica acta. Gene regulatory mechanisms. 2019 Nov 07.
• 1. Talkish J
  2. Igel H
  3. Hunter O
  4. Horner SW
  5. Jeffery NN
  6. Leach JR
  7. Jenkins JL
  8. Kielkopf CL
  9. Ares M
  Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interaction.; RNA (New York, N.Y.). 2019 May 20.
• 1. Kennedy SD
  2. Bauer WJ
  3. Wang W
  4. Kielkopf CL
  Dynamic stacking of an expected branch point adenosine in duplexes containing pseudouridine-modified or unmodified U2 snRNA sites.; Biochemical and biophysical research communications. 2019 Feb 20.
• 1. Loerch S
  2. Leach JR
  3. Horner SW
  4. Maji D
  5. Jenkins JL
  6. Pulvino M
  7. Kielkopf CL
  The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface.; The Journal of biological chemistry. 2018 Dec 19.
• 1. Kielkopf CL
  Insights from structures of cancer-relevant pre-mRNA splicing factors.; Current opinion in genetics & development; Vol 48. 2017 Nov 09.
• 1. Glasser E
  2. Agrawal AA
  3. Jenkins JL
  4. Kielkopf CL
  Cancer-Associated Mutations Mapped on High-Resolution Structures of the U2AF2 RNA Recognition Motifs.; Biochemistry. 2017 Sep 01.
• 1. Jenkins JL
  2. Kielkopf CL
  Splicing Factor Mutations in Myelodysplasias: Insights from Spliceosome Structures.; Trends in genetics : TIG. 2017 Mar 31.
• 1. Chatrikhi R
  2. Wang W
  3. Gupta A
  4. Loerch S
  5. Maucuer A
  6. Kielkopf CL
  SF1 Phosphorylation Enhances Specific Binding to U2AF(65) and Reduces Binding to 3'-Splice-Site RNA.; Biophysical journal; Vol 111(12). 2016 Dec 20.
• 1. Loerch S
  2. Kielkopf CL
  Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.; RNA (New York, N.Y.); Vol 22(12). 2016 Dec.
• 1. Fei DL
  2. Motowski H
  3. Chatrikhi R
  4. Prasad S
  5. Yu J
  6. Gao S
  7. Kielkopf CL
  8. Bradley RK
  9. Varmus H
  Wild-Type U2AF1 Antagonizes the Splicing Program Characteristic of U2AF1-Mutant Tumors and Is Required for Cell Survival.; PLoS genetics; Vol 12(10). 2016 Oct.
• 1. Agrawal AA
  2. Salsi E
  3. Chatrikhi R
  4. Henderson S
  5. Jenkins JL
  6. Green MR
  7. Ermolenko DN
  8. Kielkopf CL
  An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.; Nature communications; Vol 7. 2016.
• 1. Loerch S
  2. Kielkopf CL
  Dividing and Conquering the Family of RNA Recognition Motifs: A Representative Case Based on hnRNP L.; Journal of molecular biology; Vol 427(19). 2015 Sep 25.
• 1. Okeyo-Owuor T
  2. White BS
  3. Chatrikhi R
  4. Mohan DR
  5. Kim S
  6. Griffith M
  7. Ding L
  8. Ketkar-Kulkarni S
  9. Hundal J
  10. Laird KM
  11. Kielkopf CL
  12. Ley TJ
  13. Walter MJ
  14. Graubert TA
  U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing.; Leukemia; Vol 29(4). 2015 Apr.
• 1. Agrawal AA
  2. McLaughlin KJ
  3. Jenkins JL
  4. Kielkopf CL
  Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.; Proceedings of the National Academy of Sciences of the United States of America; Vol 111(49). 2014 Dec 9.
• 1. Loerch S
  2. Maucuer A
  3. Manceau V
  4. Green MR
  5. Kielkopf CL
  Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex.; The Journal of biological chemistry; Vol 289(25). 2014 Jun 20.
• 1. Jenkins JL
  2. Agrawal AA
  3. Gupta A
  4. Green MR
  5. Kielkopf CL
  U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs.; Nucleic acids research; Vol 41(6). 2013 Apr 1.
• 1. Gleghorn ML
  2. Gong C
  3. Kielkopf CL
  4. Maquat LE
  Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay.; Nature structural & molecular biology; Vol 20(4). 2013 Apr.
• 1. Wang W
  2. Maucuer A
  3. Gupta A
  4. Manceau V
  5. Thickman KR
  6. Bauer WJ
  7. Kennedy SD
  8. Wedekind JE
  9. Green MR
  10. Kielkopf CL
  Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex.; Structure (London, England : 1993); Vol 21(2). 2013 Feb 5.
• 1. Jenkins JL
  2. Laird KM
  3. Kielkopf CL
  A Broad range of conformations contribute to the solution ensemble of the essential splicing factor U2AF(65).; Biochemistry; Vol 51(26). 2012 Jul 3.
• 1. Bauer WJ
  2. Heath J
  3. Jenkins JL
  4. Kielkopf CL
  Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1.; Journal of molecular biology; Vol 415(4). 2012 Jan 27.
• 1. Gupta A
  2. Kielkopf CL
  Purification, crystallization and preliminary X-ray crystallographic analysis of a central domain of human splicing factor 1.; Acta crystallographica. Section F, Structural biology and crystallization communications; Vol 67(Pt 4). 2011 Apr 01.
• 1. McLaughlin KJ
  2. Jenkins JL
  3. Kielkopf CL
  Large favorable enthalpy changes drive specific RNA recognition by RNA recognition motif proteins.; Biochemistry; Vol 50(9). 2011 Mar 8.
• 1. Gupta A
  2. Jenkins JL
  3. Kielkopf CL
  RNA induces conformational changes in the SF1/U2AF65 splicing factor complex.; Journal of molecular biology; Vol 405(5). 2011 Feb 04.
• 1. McLaughlin KJ
  2. Strain-Damerell CM
  3. Xie K
  4. Brekasis D
  5. Soares AS
  6. Paget MS
  7. Kielkopf CL
  Structural basis for NADH/NAD+ redox sensing by a Rex family repressor.; Molecular cell; Vol 38(4). 2010 May 28.
• 1. Jenkins JL
  2. Shen H
  3. Green MR
  4. Kielkopf CL
  Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65.; The Journal of biological chemistry; Vol 283(48). 2008 Nov 28.
• 1. Manceau V
  2. Kielkopf CL
  3. Sobel A
  4. Maucuer A
  Different requirements of the kinase and UHM domains of KIS for its nuclear localization and binding to splicing factors.; Journal of molecular biology; Vol 381(3). 2008 Sep 5.
• 1. Lin Y
  2. Kielkopf CL
  X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.; Biochemistry; Vol 47(20). 2008 May 20.
• 1. Kumar AO
  2. Swenson MC
  3. Benning MM
  4. Kielkopf CL
  Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution.; Biochemical and biophysical research communications; Vol 367(4). 2008 Mar 21.
• 1. Swenson MC
  2. Paranawithana SR
  3. Miller PS
  4. Kielkopf CL
  Structure of a DNA repair substrate containing an alkyl interstrand cross-link at 1.65 A resolution.; Biochemistry; Vol 46(15). 2007 Apr 17.
• 1. Thickman KR
  2. Sickmier EA
  3. Kielkopf CL
  Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif.; Journal of molecular biology; Vol 366(3). 2007 Feb 23.
• 1. Sickmier EA
  2. Frato KE
  3. Shen H
  4. Paranawithana SR
  5. Green MR
  6. Kielkopf CL
  Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65.; Molecular cell; Vol 23(1). 2006 Jul 7.
• 1. Sickmier EA
  2. Frato KE
  3. Kielkopf CL
  Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.; Acta crystallographica. Section F, Structural biology and crystallization communications; Vol 62(Pt 5). 2006 May 1.
• 1. Thickman KR
  2. Swenson MC
  3. Kabogo JM
  4. Gryczynski Z
  5. Kielkopf CL
  Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors.; Journal of molecular biology; Vol 356(3). 2006 Feb 24.
• 1. Manceau V
  2. Swenson M
  3. Le Caer JP
  4. Sobel A
  5. Kielkopf CL
  6. Maucuer A
  Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65.; The FEBS journal; Vol 273(3). 2006 Feb.
• 1. Sickmier EA
  2. Brekasis D
  3. Paranawithana S
  4. Bonanno JB
  5. Paget MS
  6. Burley SK
  7. Kielkopf CL
  X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing.; Structure (London, England : 1993); Vol 13(1). 2005 Jan.
• 1. Kielkopf CL
  2. Lücke S
  3. Green MR
  U2AF homology motifs: protein recognition in the RRM world.; Genes & development; Vol 18(13). 2004 Jul 1.
• 1. Kielkopf CL
  2. Burley SK
  X-ray structures of threonine aldolase complexes: structural basis of substrate recognition.; Biochemistry; Vol 41(39). 2002 Oct 1.
• 1. Kielkopf CL
  2. Rodionova NA
  3. Green MR
  4. Burley SK
  A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer.; Cell; Vol 106(5). 2001 Sep 7.
• 1. Kielkopf CL
  2. Ding S
  3. Kuhn P
  4. Rees DC
  Conformational flexibility of B-DNA at 0.74 A resolution: d(CCAGTACTGG)(2).; Journal of molecular biology; Vol 296(3). 2000 Feb 25.
• 1. Kielkopf CL
  2. Erkkila KE
  3. Hudson BP
  4. Barton JK
  5. Rees DC
  Structure of a photoactive rhodium complex intercalated into DNA.; Nature structural biology; Vol 7(2). 2000 Feb.
• 1. Kielkopf CL
  2. Bremer RE
  3. White S
  4. Szewczyk JW
  5. Turner JM
  6. Baird EE
  7. Dervan PB
  8. Rees DC
  Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove.; Journal of molecular biology; Vol 295(3). 2000 Jan 21.
• 1. Kielkopf CL
  2. White S
  3. Szewczyk JW
  4. Turner JM
  5. Baird EE
  6. Dervan PB
  7. Rees DC
  A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA.; Science (New York, N.Y.); Vol 282(5386). 1998 Oct 2.
• 1. Kielkopf CL
  2. Baird EE
  3. Dervan PB
  4. Rees DC
  Structural basis for G.C recognition in the DNA minor groove.; Nature structural biology; Vol 5(2). 1998 Feb.