Big Bird - Amnis ImageStreamX MkII
Page under development!
URMC and the FCR has a long history with imaging cytometry dating all the way back to Leon Wheeless' work in the 70s. The most recent Amnis ImageStreamX MkII imaging flow cytometer was brought in with the support of NYFIRST grant #134,351. Originally the IS100 was sponsored by the James P. Wilmot Cancer Center, and the acquisition and upgrade to the ISX was supported by NIH Grant 1 S10 RR023458-01A1 (C Jordan PI).
Supported in part by the University of Rochester Developmental Center for AIDS Research grant P30 AI078498 (NIH/NIAID) and the University of Rochester School of Medicine and Dentistry.
D-CFAR web page
Amnis ImageStream, a hybrid fluorescent microscope/flow cytometer. With this technology, one can collect morphological data on cells stained with up to ten different fluorochromes at rates up to 50,000 events per minute. Coupled with the analytical packages, IDEAS and machine learning algorithms, statistical rigor can be brought to answer questions such as:
- validation of traditional flow results
- sub-cellular protein localization
- surface maker co-localization
- marker asymmetry
- molecular trafficking
- Phagocytosis ll
Configuration Overview
Lasers include 405nm, 488nm, 561nm, and 642 nm and there are 12 camera channels. Channels 1-6 are primarily used for the 488nm laser. Channels 7-8 are primarily for the 405 laser, channels 9-10 for the 561nm laser, and 11-12 for the 642nm laser. There are multiple channels that have multiple or cross laser excitation that compensation will take care of. On the Fluorochrome List at the bottom of the page, the common dyes visible in each channel are listed, along with the secondary laser excitation.
To design a panel on your own using Fluorofinder please click here.
General Information and links
- Additional full spectrum cytometry and instrument specific information can be found at the links below.