URMC / Research / Flow Cytometry / Services / Instruments / Analytical / Janice

Janice and Zoot - BD Symphony A1 - FACSDiva based

Note that Janice and Zoot have matching configurations and are therefore share a website page.

MIFLOWCYT information specific for this instrument can be found in the FCC_Library.

Detailed information about the QC can be found in the baseline report (available upon request).

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

To design a panel using Fluorofinder please click here.

Training Videos can be accessed here.

Configuration: Low starting voltages as of Jan 2023 posted at the machine and available via FCC_Library.

BLUE 488 LASER (100mW):

Detector	LP	Band Pass	Fluor's (check Fluorofinder for more info)
BLUE B	690	710/50	PerCP-Cy5.5
BLUE C	505	530/30	FITC, GFP, Alexa 488
BLUE E		488/10	SSC
BLUE F		488/10	Small Particle SSC
FSC

RED 637 LASER (100mW):

Detector	LP	Band Pass	Fluor's (check Fluorofinder for more info)
RED A	750	780/60	APC-Cy7, APC-Alexa 750
RED B	690	710/50	APC-Cy5.5, Alexa 700, Alexa 680
RED C	655	670/30	APC, Alexa 647

VIOLET 405 LASER (100mW):

Detector	LP	Band Pass	Fluor's (check Fluorofinder for more info)
VIOLET A	750	780/60	BV 786
VIOLET B	690	710/50	BV 711
VIOLET C	655	670/30	BV 650
VIOLET D	595	610/20	BV 605
VIOLET E	505	525/50	Pacific Orange, BV 510,
VIOLET F	410	431/28	Pacific Blue, BV 421

YELLOW-GREEN 561 LASER (100mW):

Detector	LP	Band Pass	Fluor's (check Fluorofinder for more info)
YG A	750	780/60	PE-Cy7, PE-Alexa 750
YG B	690	710/50	PE-Cy5.5
YG C	655	670/30	7AAD, PE-Cy5
YG D	595	610/20	PE-Alexa 610, PE-Texas Red
YG E	580	586/15	PE, Alexa 532