URMC / Research / Flow Cytometry / Services / Instruments / Analytical / Kermit

Kermit : This instrument is currently disabled

Suggested voltages current as of Sept, 2019.

Detailed information about this instrument can be found on the baseline report (available upon request).

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

To design a panel using Fluorofinder please click here.

LSR-II Configuration:

BLUE 488 LASER (20mW):

Detector	LP	Band Pass	Fluor's	Low Voltage
BLUE A	735	780/60	PE-Cy7, PE-Alexa750	500
BLUE B	685	710/50	PerCP-Cy5.5, PE-Cy5.5, PerCP, PE-Alexa700	550
BLUE C	665	660/20	PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++	550
BLUE D	600	610/20 630/30	PI, PE-Texas Red, PE-Alexa610, PE-Alexa594	500
BLUE E	550	576/26	PE	550
BLUE F	505	525/50	FITC, Alexa488, YFP, GFP, CFSE	400
BLUE G		488/10	SSC

RED 633 LASER (20mW):

VIOLET 405 LASER (40mW):

Detector	LP	Band Pass	Fluor's	Low Voltage
VIOLET A	595	610/20	BV 605	400
VIOLET B	505	525/50	Fura-High_Ca++, Pacific Orange	350
VIOLET C		450/50	AlexaFluor 405, DAPI, BV 421, Pacific Blue	300