Histology Forms and Protocols
Internal Forms
Internal Work Order Form – Histology
General Protocols
Histology Guidelines Presentation
Tissue Submission for Paraffin Processing
Preparation of Frozen Tissue Specimens
Embedding Frozen Tissues
Slide Preparation
HBMI Core Decalcification Solutions
Histology Staining Protocols
Hematoxylin and Eosin Stain
Alcian Blue/Orange G Stains
Safranin O/Fast Green
Toluidene Blue/Fast Green
TRAP stain for Paraffin Sections
Beta Galactosidase Staining - Frozen Tissue Sections
Von Kossa Staining
Brown-Brenn Modified Gram Staining
Masson Trichrome Stain
PAS (Periodic Acid Schiff) Staining
FAST Stain
Historic IHC/IF Protocols
Aggrecan - IHC
Aggrecan (NITEGE) - IHC
Aggrecan (DIPEN) - IHC
Anti-PTH/PTHrP Receptor - IHC
Beta Galactosidase (LacZ) - IHC
Beta Catenin - IHC
Beta Catenin (Active) - IHC
BMP-2 - IHC
Cadherin11 - IHC
Collagen Type 1a1 - IHC
Collagen Type 2a1 – IHC
Collagen Type 3a1 - IHC
Collagen Type 10a1 – IHC
F4/80 - IF
GFP - IHC
Lubricin (Prg4) - IHC
Matrix Metalloproteinase 13 – IHC
P27 - IHC
PECAM (CD31) - IHC
PPAR Gamma - IHC
Perilipin - IHC
Phosphorylated SMAD 1/5/8 – IHC
RANK - IHC
Runx2 - IHC
Sox-9 - IHC
Tak1 - IHC
TNF alpha - IHC
TRAP - IHC
VEGF - IHC
VEGF Receptor 2 - IHC
ISH Protocols
In Situ Hybridization – Radiolabeled 35S
In Situ Hybridization - Digoxigenin