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Flow Cytometry

URMC / Research / Flow Cytometry / Services / Instruments / Cell Sorting / BOOKING A SORT

BOOKING A SORT

SORT BOOKING PROCESS

  1. Users will Book Time on PPMS 
    1. Users are required to reserve their desired time slots in PPMS a minimum of 48 hours prior to the intended start time.
    2. Investigators that need to sort within 48 hours of the intended start must call FCR Staff at 276-5330 or send an email requesting the desired date and time. A voicemail is not sufficient. The FCR Staff will review your request and if it can be accommodated, they will add it to PPMS for you.  Sort time is not guaranteed in this situation.
    3. User must book a minimum of 1 hour for their sort. 
    4. Reservations on PPMS have a booking form to designate nozzle size for your sort. Please fill in the correct nozzle size here and make sure it matches your sort form to ensure that the instrument is prepared for your sort. 
    5. Once booked the desired time slot will show up as red (pending) in PPMS until it has been approved by FCR Staff.
    6. If needed, use the Scheduling Considerations and Table 1 to help determine how much time you to book for your sort. Please contact FCR staff and inquire if you are unsure.
  2. Users will then submit their Sort Form in SmartSheet
    1. Once you have booked your sort time on PPMS, you must submit your sort form, at least 48 hours in advance of your sort or we cannot guarantee your sort will be conducted.  
    2. For help with this process, please click here.  
  3. FCR Staff will Review the Sort Form in SmartSheet
    1. Once submitted a FCR Staff member will review your Sort Form and reach out if they have any questions or concerns.  This typically happens within 1-2 business days of submission. 
  4. FCR Staff will Approve the Booked Time in PPMS
    1. Once your sort form has been reviewed and any concerns have been addressed then FCR Staff will confirm your time and it will turn blue (approved) in PPMS.

SCHEDULING CONSIDERATIONS

  • Investigators new to sorting are encouraged to schedule an appointment to discuss their sorting needs with a member of the FCR staff prior to booking sort time.
  • When scheduling time, please allow for up to 30 minutes for instrument and software setup. If you are sorting with us for the first time or piloting a new experiment, you may want to book additional time depending on the size of your panel and the number of controls that you have to run. Please contact FCR staff and inquire if you are unsure.
  • When sorting yeast, bacteria or live virus, please book an additional 30 minutes to allow for extra cleaning before and after the sort.
  • For investigators using the 130 nozzle, please book an additional 30 minutes for nozzle changes as switching to the 130 micron nozzle can take up to 30 minutes for the fluid stream to stabilize. 

 **FEE STRUCTURE**

  • Sort rates include a setup fee and an hourly charge with a 1 hour minimum; time is then billed to the minute. Time billed will be time reserved unless the reservation runs long, then the extra time will be added to the bill. Bills are sent out at the end of the month. Please direct all billing questions to Matt Cochran.
  • Investigators that cancel less than 48 hours before the start of the scheduled sort time will be charged for the time unless the reservation is after 5pm, in which case a charge will be applied.